The Effect of Dissolution Condition on the Yield, Molecular Weight, and Wet- and Electro-spinnability of Regenerated Silk Fibroins Prepared by LiBr Aqueous Solution

  • Cho, Hee-Jung (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University) ;
  • Um, In-Chul (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University)
  • Received : 2010.06.08
  • Accepted : 2010.06.20
  • Published : 2010.06.30

Abstract

In this paper, the regenerated silk fibroins were dissolved in LiBr aqueous solution with different dissolution temperature and time, and the effects of the dissolution condition on the regeneration yield, molecular weight, wet spinnability, and electrospinnability of regenerated silk fibroin were investigated. The regeneration yield, molecular weight distribution, and wet spinnability of regenerated silk fibroin were nearly affected by the dissolution temperature and time. However, the electrospinning performance of silk fibroin was influenced by the dissolution condition implying the electrospinning of silk fibroin is more sensitive process than the wet spinning in the range tested in this study. While $25^{\circ}C$ of dissolution temperature resulted in a good electrospinnability of regenerated silk fibroin, the electrospinnability was slightly deteriorated when silk fibroin was dissolved at $60^{\circ}C$ for 6 hours. Also, though the fiber diameters of electrospun silk fibroin produced by the dissolution at $25^{\circ}C$ for 6 hours and 24 hours were 443 and 451 nm, respectively, that at $60^{\circ}C$ for 5 min was reduced to 411 nm. The fiber diameter was more decreased to 393 nm when the dissolution time increased up to 6 hours at $60^{\circ}C$.

Keywords

References

  1. Bhardwaj N, Kundu SC (2010) Electrospinning : A fascinating fiber fabrication technique. Biotech Adv 28, 325-347. https://doi.org/10.1016/j.biotechadv.2010.01.004
  2. Freddi G, Romano M, Massafra MR, Tsukada M (1995) Silk fibroin/cellulose blend films: preparation, structure, and physical properties. J Appl Polym Sci 56, 1537-1545. https://doi.org/10.1002/app.1995.070561203
  3. Ishizaka H, Watanabe Y, Ishida K, Fukumoto O (1989) Regenerated silk prepared from ortho phosphoric acid solution of fibroin. J Seric Sci Jpn 58, 87-95.
  4. Ki CS, Park SY, Kim HJ, Jung HM, Woo KM, Lee JW, Park YH (2008) Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Biotech lett 30, 405-410. https://doi.org/10.1007/s10529-007-9581-5
  5. Min B, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25, 1289-1297. https://doi.org/10.1016/j.biomaterials.2003.08.045
  6. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrixes. J Biomed Mater Res 29, 1215-1221. https://doi.org/10.1002/jbm.820291008
  7. Sakabe H, Ito H, Miyamoto T, Noishiki, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen-i Gakkaishi 45, 487-490. https://doi.org/10.2115/fiber.45.11_487
  8. Sukigara S, Gandhi M, Ayutsede J, Micklus M, Ko F (2003) Regeneration of Bombyx mori silk by electrospinning. part 1: processing parameters and geometric properties. Polymer 44, 5721-5727. https://doi.org/10.1016/S0032-3861(03)00532-9
  9. Um IC, Cho HJ, Kim JW, Park YH, Kweon HY (2010) Effect of storage time on the wet-spinning and electro-spinning of various molecular weight silk formic acid solutions. 239th ACS national meeting, San Francisco, Tech-92.
  10. Um IC, Ki CS, Kweon H, Lee GK, Ihm DW, Park YH (2004) Wet spinning of silk polymer: II. Effect of drawing on the structural characteristics and properties of filament. Int J Biol Macromol 34, 107-119. https://doi.org/10.1016/j.ijbiomac.2004.03.011
  11. Um IC, Park YH (1998) Morphology of silk fibroin/poly(vinyl alcohol) blend film. Korean J Seric Sci 40, 169-175.
  12. Wang Y, Bella E, Lee CSD, Migliaresi C, Pelcastre L, Schwartz Z, Boyan BD, Motta A (2010) The synergistic effects of 3-D porous silk fibroin matrix scaffold properties and hydrodynamic environment in cartilage tissue regeneration, Biomaterials 17, 4672-4681.
  13. Wang Y, Kim H, Vunjak-Novakovic G, Kaplan DL (2006) Stem cell-based tissue engineering with silk biomaterials. Biomaterials 27, 6064-6082. https://doi.org/10.1016/j.biomaterials.2006.07.008
  14. Zhu J, Zhang Y, Shao H, Hu X (2008) Electrospinning and rheology of regenerated Bombyx mori silk fibroin aqueous solutions: The effects of pH and concentration. Polymer 49, 2880-2885. https://doi.org/10.1016/j.polymer.2008.04.049