Profiles of Glucocorticoid Receptor mRNA Expression and Physiological Changes in Response to Osmotic and Thermal Stress Conditions in Black Porgy (Acanthopagrus schlegeli)

염분과 수온 스트레스에 따른 감성돔의 glucocorticoid receptor mRNA 발현 특징과 생리적 변화에 관한 연구

  • An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Shin, Hyun-Suk (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Kil, Gyung-Suk (Division of Electrical & Electronic Engineering, Korea Maritime University) ;
  • Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 안광운 (한국해양대학교 해양환경.생명과학부) ;
  • 신현숙 (한국해양대학교 해양환경.생명과학부) ;
  • 민병화 (국립수산과학원 육종연구센터) ;
  • 길경석 (한국해양대학교 전기전자공학부) ;
  • 최철영 (한국해양대학교 해양환경.생명과학부)
  • Received : 2010.02.22
  • Accepted : 2010.03.23
  • Published : 2010.03.31

Abstract

The present study investigated the expression of glucocorticoid receptor (GR) mRNA as a stress response during salinity changes (35, 10, and 0 psu) and water temperature changes (from $20^{\circ}C$ to $30^{\circ}C$, $1^{\circ}C$/day) in black porgy. We cloned the full-length GR cDNA from the kidney and examined its expression in the gill, kidney, and intestine by quantitative real-time PCR (QPCR) during salinity and water temperature changes. During salinity changes, the levels of GR mRNA in the gill, kidney, and intestine were highest at 0 psu, and the levels of plasma cortisol and glucose were elevated, but triiodothyronine ($T_3$) decreased. Also, during water temperature changes, the levels of GR mRNA in the gill, kidney, and intestine increased at $30^{\circ}C$. Plasma parameters also increased with an increase in water temperature. Therefore, this upregulation of GR mRNA was a good indicator of stress, such as those resulting from changes in salinity and water temperature.

본 연구에서는 감성돔의 염분과 수온 변화에 따른 스트레스 반응을 알아보기 위하여 glucocorticoid receptor (GR) mRNA 발현을 조사하였다. 감성돔 신장으로부터 전장의 GR cDNA를 클로닝하였고, 염분과 수온이 변화하는 동안 아가미, 신장 및 장에서 GR mRNA 발현 변화를 quantitative real-time PCR (QPCR)을 이용하여 조사하였다. 염분 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 0 psu에서 가장 높게 나타났으며, 혈장 cortisol과 glucose 농도도 증가한 반면, triiodothyronine ($T_3$) 농도는 감소하였다. 수온 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 $30^{\circ}C$에서 가장 높게 관찰되었다. 혈장 cortisol, glucose 및 $T_3$ 농도 또한 고수온 ($30^{\circ}C$)에서 증가하였다. GR mRNA 발현의 증가는 염분과 수온 변화와 같은 환경 요인에 대한 좋은 스트레스 지표로 여겨진다.

Keywords

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