Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-lipase and Lipolytic Activities of EtOH Extract from Juniperus rigida

노간주나무 에탄올추출물의 지방 흡수 억제 및 지방분해 효능

  • Lee, Young-Seop (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Jung-Hhyun (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Hyo-Jun (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Sohn, Eun-Jin (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Chan-Sik (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Jeong, Il-Ha (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Jo, Kyu-Hyung (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Joo-Hwan (Department of Life Science, Kyungwon University) ;
  • Kim, Jin-Sook (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM))
  • 이영섭 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김정현 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김효준 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 손은진 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김찬식 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 정일하 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 조규형 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김주환 (경원대학교 생명과학과) ;
  • 김진숙 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터)
  • Received : 2010.07.28
  • Accepted : 2010.09.08
  • Published : 2010.09.30
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Abstract

Obesity is an important risk factor that significantly increases mortality and disease rates in the cardiovascular disease, diabetes, and various diseases. So far, the most powerful way to inhibit fat absorption is pancreatic lipase inhibitors. In this study, we investigated the anti-obesity effect of the extract of Juniperus rigida. Juniperus rigida extract (JRE) had a inhibitory effect on pancreatic lipase activity ($IC_{50}$=8.63 ${\mu}g$/ml). In in vivo oil-emulsion loading test, this extract also inhibited the intestinal fat absorption. In addition, we measured inhibitory effects of JRE on activity of phosphodiesterase (PDE) and hormone sensitive lipase (HSL) among the important enzymes associated with lipolysis. JRE strongly inhibited PDE activity ($IC_{50}$=4.56 ${\mu}g$/ml), whereas inhibitory effect on HSL activity was very weak compared with orlistat. As a result, JRE inhibited the absorption of fat by inhibiting the activity of pancreatic lipase and induced lipolysis through inhibition of PDE activity. Therefore, we suggest that Juniperus rigida may be a potential therapeutic agent improving obesity.

Keywords

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