약학회지 (YAKHAK HOEJI)

  • 제54권1호
  • /
  • Pages.22-26
  • /
  • 2010
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

머루 줄기와 자소자로부터 분리한 Resveratrol 올리고머와 Flavonoid의 항균효과

Antimicrobial Activity of Resveratrol Oligomers and Flavonoids from the Stems of Vitis coignetiae Pulliat and the Seeds of Perilla frutescens (L.) Britton

  • 손락호 (정산생명공학연구소) ;
  • 진휘승 (정산생명공학연구소) ;
  • 함아름 (서울대학교천연물과학연구소) ;
  • 마응천 (서울대학교천연물과학연구소) ;
  • 남궁우 (정산생명공학연구소)
  • Son, Rak-Ho (R&D Center, Jung San Bio Technology) ;
  • Chin, Hwi-Seung (R&D Center, Jung San Bio Technology) ;
  • Ham, Ah-Rom (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Nam, Kung-Woo (R&D Center, Jung San Bio Technology)
  • 투고 : 2009.09.09
  • 심사 : 2009.12.08
  • 발행 : 2010.02.28
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초록

We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, $\varepsilon$-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration ($IC_{50}$) of compound 2 against S. aureus was 7.2 ${\mu}M$. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 ${\mu}M$, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.

키워드

참고문헌

  1. Cherry, J. P. : Improving the safety of fresh produce with antimicrobials. Food Technol. 53, 54 (1999).
  2. Chang, D. S., Shin, D. H., Chung, D. H., Kim, C. M. and Lee, I. S. : Food hygiene. Jungmoongak, Seoul. p. 244 (2002).
  3. Ferrand, C., Marc, F. and Fritsch, P. : Chemical and toxicological studies of products resulting from sorbic acid and methylamine interaction in food conditions. Amino Acids. 18, 251 (2000). https://doi.org/10.1007/s007260050022
  4. Lee, D. H., Yu, H. E. and Lee, J. S. : Quality characteristics and physiological functionality of wild grape wine. J. Natural. Sci. 15, 69 (2004).
  5. Chonbuk University. : Development of tree shape, fruiting management and processing technology of coating rice in Gailiangmeru (Vitis spp.). 2005 Report of Ministry of Agriculture and Forestry, p. 3 (2005).
  6. Pace-Asciak, C. R., Hahn, S., Diamandis, E. P., Soleas, G. and Goldberg, D. M. : The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: Implications for protection against coronary heart disease. Clin. Chim. Acta. 235, 207 (1995). https://doi.org/10.1016/0009-8981(95)06045-1
  7. Seo, S. H., Lee, H. L., Lee, S. J., Choe, S. W. and Jo, S. H. : Effects of paeonia seed extracts and resveratrol on lipid metabolism in ratsfed high cholesterol diets. J. Korean Soc. Food. Sci. Nutr. 32, 1102 (2003). https://doi.org/10.3746/jkfn.2003.32.7.1102
  8. Fremont, L., Belguendouz, L. and Delpal, S. : Antioxidant activity of resveratrol and alcohol-free wine polyphenols realated to LDL oxidation and polyunsaturated fatty acids. Life Sci. 64, 2511 (1999). https://doi.org/10.1016/S0024-3205(99)00209-X
  9. MacCarrone, M., Lorenzon, T., Guerrieri, P. and Agro, A. F. : Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. Eur. J. Biochem. 265, 27 (1999). https://doi.org/10.1046/j.1432-1327.1999.00630.x
  10. Lee, H. S., Sur, E. Y. and Kim, W. K. : Resveratrol induces apoptosis in SW480 human colon cancer cell lines. Food Sci. Biotechnol. 13, 80 (2004).
  11. Fontecave, M., Lepoivre, M., Elleingand, E., Geres, C. and Guittet, O. : Resveratrol, a remarkable inhibitor of ribonucleotide reductase. FEBS Lett. 421, 277 (1998). https://doi.org/10.1016/S0014-5793(97)01572-X
  12. Gehm, B. D., McAndrews, J. M., Chien, P. Y. and Jameson, J. L. : Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Proc. Natl. Acad. Sci. U.S.A. 94, 14138 (1997). https://doi.org/10.1073/pnas.94.25.14138
  13. Chol, S. G. and Lee, J. I. : Effects of Planting Dates on Major Agronomic Characteristics and Yield of Perilla frutescens var. acuta. Kupo Personal Authors. 36, 143 (1991).
  14. Park, H. J., Chung, D. H., Kim, S. G. and Kwon, B. S. : Influences of sowing time and nursery period on growth and yield of Perilla frutescens BRITTON var. acuta KUDO. Korean J. Crop. Sci. 3, 1 (1995).
  15. 배기환 : 한국의 약용식물. 교학사, 서울 p. 441 (2000).
  16. Nakazawa, T., Yasuda, T., Ueda, J. and Ohsawa, K. : Antidepressant-like effects of apigenin and 2,4,5-trimethoxycinnamic acid from Perilla frutescens in the forced swimming test. Biol. Pharm. Bull. 26, 474 (2003). https://doi.org/10.1248/bpb.26.474
  17. Ishikura, N. : Anthocyanins and flavones in leaves and seeds of Perilla plant. Agric. Biol. Chem. 45, 1855 (1981). https://doi.org/10.1271/bbb1961.45.1855
  18. Yamamoto, H. and Ogawa, T. : Antimicrobial activity of Perilla seed polyphenols against oral pathogenic bacteria. Biosci. Biothechnol. Biochem. 66, 921 (2002). https://doi.org/10.1271/bbb.66.921
  19. Nakamura, S., Kato, A. and Kobayashi, K. : New antimicrobial characteristics of lysozyme-dextran conjugate. J. Agrc. Food Chem. 39, 647 (1991). https://doi.org/10.1021/jf00004a003