Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Stimulation of the Immune Response by Herbal Formulas for Wind-Cold and Heat Pain Symptom

대표적인 풍한열(風寒熱)에 의한 통증 치료 처방의 면역 활성화 비교 연구

  • Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 정다영 (한국한의학연구원 한약EBM연구센터) ;
  • 하혜경 (한국한의학연구원 한약EBM연구센터) ;
  • 이호영 (한국한의학연구원 한약EBM연구센터) ;
  • 이진아 (한국한의학연구원 한약EBM연구센터) ;
  • 이준경 (한국한의학연구원 한약EBM연구센터) ;
  • 황대선 (한국한의학연구원 한약EBM연구센터) ;
  • 신현규 (한국한의학연구원 한약EBM연구센터)
  • Received : 2010.06.14
  • Accepted : 2010.08.02
  • Published : 2010.08.25
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Abstract

Three herbal formulas (Bangpungtongsung-san, Ohyaksungi-san, and Ojeok-san) for wind-cold and heat pain symptom were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium ($100\;{\mu}g/200\;{\mu}g$/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, lgG1, and total IgM classes) in plasma were measured. Ohyaksungi-san increased NO synthesis in RAW 264.7 cells. Ojeok-san and Ohyaksungi-san significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Three herbal formulas for wind-cold and heat pain symptom also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. These results suggested that three herbal formulas for wind-cold and heat pain symptom could be used as stimulator of immune response.

Keywords

References

  1. Lim, E.M., Lee, S.H. The meaning of immunity in the documents of oriental medicine. Journal of the Korea institute of oriental medical informatics. 12(1):129-145, 2006.
  2. Hur, S.M., Kwon, C.H., Joh, J.W., Kim, S.J. Alteration of molecules and cytokines related to the activation of T lymphocyte in immune tolerance induced mice model. J Korean Surg Soc. 19(2):119-123, 2005.
  3. Seo, Y.B., Kil, G.J., Lee, Y.C., Kim, D.H. Experimental studies on thymus cells differentiation by the extracts of Stigma Croci. Kor J Herbology. 18(3):97-111, 2003.
  4. Jang, S.J., Chun, H.N., Yun, S.S., Lee, I.S., Lee, Y.S. Effects of deer antler extract on serum IGF-I, bone growth and splenocyte proliferation in growing rats. Korean J Nutr. 39(3):225-235, 2006.
  5. Friedl, R., Moeslinger, T., Kopp, B. and Spiecke-rmann, P.G. Stimulation of nitric oxide synthesis by the aqueous extract of Panax ginsena root in Raw 264.7 cells. Br J pharmacol. 134: 1663-1670, 2001. https://doi.org/10.1038/sj.bjp.0704425
  6. Farrell, A.J., Blake, D.R. Nitric oxide. Annals of the Rheumatic Dieases. 55: 7-20, 1996. https://doi.org/10.1136/ard.55.1.7
  7. Ryu, H.S., Kim, J., Park, S.C., Kim, H.S. Enhancing effect of Zingiber Officinale Roscoe extract on mouse spleen and macrophage cells activation. Korean J of Nutrition. 37(9):780-785, 2004.
  8. Sun, J., Hu, S., Song, X. Adjuvant effects protopanaxadiol and protopanaxatriol saponins from ginseng roots on the immune reponses to ovalbumin in mice. Vaccine. 25: 1114-1120, 2007. https://doi.org/10.1016/j.vaccine.2006.09.054
  9. Dep. of oriental medicine prescription in 11 oriental medicine college. Text of prescription, Seoul, Younglimsa, pp 258-270, 2003.
  10. Jeong, W.J., Choi, D.Y., Nam, S.S., Lee, J.D., Ahn, B.C., Park, D.S., Lee, Y.H., Choi, Y.T. The study on pain in Huangdi-Neijing. The Korean Acupuncture & Moxibustion Society. 14(2):149, 1977.
  11. Hwang Doyeon, Bangyakhappyeon. Seoul, Namsandang, p 132, 2003.
  12. Kim, H.J., Yoon, K.M., Im, E.Y., Byun, J.S., Kim, D.J., Kwak, M.N. Three case report of Bangpungtongsung-san effect on improvement of hypertension patients. Korea J Oriental Physiology & Pathology. 23(3):740-743, 2009.
  13. Shin, D.H., Cho, G.H., Lee, H., Moon, M.K., Kang, D.G., Yun, Y.G., et al. Clinical study of Bangpoongtongsungsan on body weight change in subjects with obesity. Korean J Oriental Med Prescrip. 16(2):133-144, 2008.
  14. Choi, E.M., Ryu, E.K. Effects of Bang-Pung-Tong-Seoung-San on obese patients. J Society Korean Med for Obesity Research. 1(1):1-11, 2001.
  15. Kim, H.J., Kim, Y.B., Park, O.S., Kim, K.S., Cha, J.H. The effect of Bangpungtongsung-san on model of allergic rhinitis. J Korean Oriental Med Ophthalmology & Otolaryngology & Dermatology. 19(1):21-30, 2006.
  16. Son, J.M., Hong, S.U. The effects of Bangpungtongsungsan extract to the skin damage on mice model after atopic dermatitis elicitation. Korean J Oriental Med Prescrip. 20(1):99-114, 2007.
  17. Ha, J.Y., Lee, S.G., Yu, B.G. Effects of Oyaksunkisan acua-acupuncture on adjuvant arthritis in rats. Korea J Oriental Physiology & Pathology. 14(2):144-154, 2000.
  18. Shin, H.S., Ko, H., Kim, H.H. Effects of Oyaksoongi-san extract on hypertention and common carotid artery. Korean J Oriental Med. 10(2):79-92, 2004.
  19. Lee, N.H., Ha, H.K., Lee, H.Y., Jung, D.Y., Choi, J.Y., Lee, J.K. et al. Analysis of studies on Ojeok-san for establishment of evidence based medicine. Korean J Oriental Med Prescrip. 16(2):1-9, 2008.
  20. Jung, S.Y., Lee, M.J. The effects of Ohyaksunki-san(Wuyaoshunqisan) on increasing body fat of rat induced by high fat diet. J Oriental Rehab Med. 17(4):23-39, 2007.
  21. Liew, F.Y., Lia, Y., Millot, S. Tumor necrosis factor synergizes with IFN-$\gamma$ in mediating killing of Leishmania major through the induction of nitric oxide. J Immunol. 145: 4306-4310, 1990.
  22. Kawamata, H., Ochiai, H., Mantani, N., Terasawa. Enhanced expreesion of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med, 28: 217-226, 2000. https://doi.org/10.1142/S0192415X0000026X
  23. Mu, M.M., Chakravortty, D., Sugiyama, T., Koide, N., Takahashi, K., Mori, I., Yoshida, T., Yokoch, T. The inhibitory action of quercetin on lipopolysacchride-induced nitric oxide prodution in RAW 264.7 macrophage cells. J Endotoxin Res. 7: 431-438, 2001. https://doi.org/10.1177/09680519010070060601
  24. Ryu, J.H., Ahn, H., Kim, J.Y., Kim, Y.K. Inibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages. Phytother Res. 17: 485-489, 2003. https://doi.org/10.1002/ptr.1180
  25. Stokes, K.Y., Cooper, D., Tailor, A., Granger, D.N. Hypercholesterolemia promotes inflammation and microvascular dysfunction: role nitric oxide and superoxide. Free Radical Biol Med. 33: 1026-1036, 2002. https://doi.org/10.1016/S0891-5849(02)01015-8
  26. Thornberry, N.A., Molineaux, S.X. Interleukin-1 beta converting enzyme: a novel cysteine protease required for IL-1 beta production and implicated in progammed cell death. Protein Sci. 4: 3-12, 2005.
  27. Burger, D., Dayer, J.M., Palmer, G., Gabay, C. Is IL-1 a good therapeutic target in the treatment of arthritis? Best Pract Clin Rheumatol. 20(5):879-896, 2006. https://doi.org/10.1016/j.berh.2006.06.004
  28. Sung, C.S., Wong, C.S. Cellular mechanisms of neuroinflammatory pain: the role of interleukin-1beta. Act Anaesthsiol Taiwan. 45(2):103-109, 2007.
  29. Cyster, J.G. Chemokines and cell migration in secondary lymphoid organs. Science. 286: 2098-2102, 1999. https://doi.org/10.1126/science.286.5447.2098
  30. Dutton, R.W., Bradley, L.M., Swain, S.L. T cell memory. Annu Rev Immnol. 16: 201-223, 1998. https://doi.org/10.1146/annurev.immunol.16.1.201
  31. Kupper, T.S. T cells, immnonsurveillance, and cutaneous immunity. J Dermatol Sci. 24: S41-45, 2000. https://doi.org/10.1016/S0923-1811(00)00140-7
  32. Zalys, R., Zagon, I.S., Bonneau, R.H., Lang, C.M., McLaughlin, P.J. In vivo effect of chronic treatment with (MET5)-enkephain on hematological values and natural killer cell activity in athymic mice. Life Sci 66: 829-834, 2000. https://doi.org/10.1016/S0024-3205(99)00655-4
  33. James, G.L. Methods in Immunotoxicology. John Wiley-Liss, Inc, San Diego. pp 2-15, 1995.
  34. Mosmamm, T.R., Coffman, R.L. TH1 and TH2 cells:different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 7: 145-173, 1989. https://doi.org/10.1146/annurev.iy.07.040189.001045
  35. Del Prete, G.F., De Carli, M., Mastromauro, C., Biagiottl, R., Macchia, D., Falagiani, P., Ricci, M., Romagnani, S. Purified protein derivative of Mycobacterium tuberculosis and excretory-secretory antigen(s) of Toxocara canis expand in vitro human T cells with stale and opposite (type 1 T helper or type 2 T helper) profile of cytokine production. J Clin Invest. 88: 346-350, 1991. https://doi.org/10.1172/JCI115300
  36. Carter, L.L., Dutton, R.W. Type 1 and type 2: a fundamental dichotomy for all T-cell subsets. Curr Opin Immunol. 8: 336-342, 1996. https://doi.org/10.1016/S0952-7915(96)80122-1
  37. Stephens, R., Eisenbarth, S.C., Chaplin, D.D. T helper ytpe 1 cells in asthma: friend or foe? Curr Opin Allergy Clin Immunol. 2: 31-37, 2002. https://doi.org/10.1097/00130832-200202000-00006
  38. Janeway, Trevers. Immunobiology. 3rd. London:Current Biology. pp 715-731, 1997.