Phylogenic Relationship of Rubus Cultivated in Korea Revealed by Chloroplast DNA Spacers

Chloroplast DNA Spacers로 분석한 국내 Rubus 재배종의 계통학적 유연관계

  • Eu, Gee-Suck (Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Park, Myoung-Ryoul (Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Baek, So-Hyeon (National Institute of Crop Science, RDA) ;
  • Yun, Song-Joong (Department of Crop Science, Institute of Agricultural Science and Technology, Chonbuk National University)
  • 유기석 (전북대학교 농업생명과학대학 농업과학기술연구소) ;
  • 박명렬 (전북대학교 농업생명과학대학 농업과학기술연구소) ;
  • 백소현 (농촌진흥청 국립식량 과학원) ;
  • 윤성중 (전북대학교 농업생명과학대학 작물생명과학과)
  • Received : 2010.05.11
  • Accepted : 2010.08.19
  • Published : 2010.08.30

Abstract

There is a considerable difference in morphological traits between Bokbunja cultivated in Korea (KCB) and Korea native Rubus coreanus, contrary to the conviction that the cultivated Bokbunja is the domestication of R. coreanus. To infer the phylogenetic relationship of KCB with other Rubus species, we compared the chloroplast DNA spacers of KCB with those of several Rubus species including black raspberry, R. occidentalis. The three chloroplast DNA spacers, atpB~rbcL, trnL~trnF, and trnT~trnL, were amplified using the specific primer pairs and converted to Single Strand Conformational Polymorphism (SSCP) markers. The SSCP makers of the chloroplast DNA spacers showed a considerable variation both within and among Rubus species. In the phylogenetic tree generated by the SSCP markers, KCB accessions were located in the same clade with R. occidentalis, but R. coreanus accessions in the different clade. Also, in the phylogenetic tree by the nucleotide sequences of the chloroplast DNA spacer trnL~trnF, KCB located in the same clade with R. occidentalis but not with R. coreanus. These results suggest that the three KCB accessions share higher similarity with R. occidentalis than with R. coreanus in the three chloroplast DNA spacers.

Keywords

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