Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Calves Derived from in Vivo Frozen-Thawed Embryos Collected from Canada Holstein Friesian Cows with High Genetic Background

캐나다산 고능력 젖소에서 생산된 동결-융해 배아 유래의 송아지 생산

  • Lee, Won-You (Embryo Research Center, Seoul Dairy Cooperative) ;
  • Lee, Woo-Sung (Embryo Research Center, Seoul Dairy Cooperative) ;
  • Kim, Hyung-Jong (Embryo Research Center, Seoul Dairy Cooperative) ;
  • Kim, Bong-Han (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Hong, So-Gun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong-Chun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
  • 이원유 (서울우유 생명공학연구소) ;
  • 이우성 (서울우유 생명공학연구소) ;
  • 김현종 (서울우유 생명공학연구소) ;
  • 김봉한 (서울대학교 수의과대학 산과학교실) ;
  • 홍소군 (서울대학교 수의과대학 산과학교실) ;
  • 이병천 (서울대학교 수의과대학 산과학교실) ;
  • 장구 (서울대학교 수의과대학 산과학교실)
  • Accepted : 2010.03.23
  • Published : 2010.04.30
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Abstract

Embryo Transfer (ET) is one of the assisted reproductive technologies and a useful tool for improving herds. The purpose of this study is to produce the calves using frozen embryos which were produced in the top one percent Holstein in Canada by ET. One hundred seventeen recipients were used for surrogate mothers and seventy cows were diagnosed to be pregnant. Fifty seven calves were born successfully and thirteen out of them failed to produce viable calves (abortion: 4, stillbirth: 9). Their gestational length, birth body weight and sex ratio for all the viable calves(n = 57) were $278.1{\pm}3.6$ days (range: from 271 to 286 days), $44.0{\pm}3.0\;kg$ (range: from 37 to 49 kg) and 57.9 vs. 42.1 % (male 33 and female 24), respectively. Microsatellite analysis confirmed that they were derived from frozen embryos. In conclusion, this study demonstrated that viable calves derived from frozen-thawed embryos from Canada were born by ET.

소에서 유전적 군 (herd)의 향상을 위한 유용한 도구는 보조 생식술 중 배아 이식 (embryo transfer)으로 알려져 왔다. 본 연구의 목적은 캐나다 유전 능력 상위 1% 이내의 젖소에서 생산된 냉동 배아를 수입하여, 국내 수란우군을 이용한 배아이식을 적용한 후, 송아지를 생산하는 것이다. 117번의 배아 이식이 이루어졌고, 그 중 70 마리에서 임신이 확인 되었으며, 총 57 마리의 송아지가 성공적으로 태어났다. 그들의 임신기간, 체중, 성별은 각각 $278.1{\pm}3.6$ 일 (범위: 271 에서 286일), $44.0{\pm}3.0\;kg$ (범위: 37 에서 49 kg) and 57.9 vs. 42.1% (수컷: 33, 암컷: 24) 이었다. 미세위성체검사를 통하여, 태어난 송아지는 모두 수입된 배아 유래임이 증명되었다. 결론적으로, 본 연구를 통하여 캐나다에서 수입된 배아로부터 살아있는 송아지를 성공적으로 얻을 수 있었으며, 이 송아지들은 앞으로 국내 낙농산업에 있어 종모우와 종빈우가 되는 좋은 유전자원이 될 것이다.

Keywords

References

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