High Prevalence of Lipid-Dependent Malassezia Infections in Dogs

개에서 조사된 높은 지방 친화성 Malassezia 감염율

  • Han, Jae-Ik (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Cho, Sang-Hee (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Na, Ki-Jeong (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 한재익 (충북대학교 동물의료센터) ;
  • 조상희 (충북대학교 동물의료센터) ;
  • 나기정 (충북대학교 동물의료센터)
  • Accepted : 2010.04.13
  • Published : 2010.04.30

Abstract

Malassezia (M.) is a member of normal mycobiota in warm-blooded vertebrates. Increased humidity is likely to be crucial in this infection. We studied the proportion of the species infected in dog during summer of Korea. Fifty samples were analyzed by PCR-RFLP and direct sequencing from June 2006 to October 2006. The study showed that lipid-dependent species was main pathogen (M. furfur; 86%, M. obtusa; 10%) while M. pachydermatis (4%) has only small portion. This result suggests that Malassezia infection has endemic characters that can be affected by the climate (temperature and humidity) in dogs.

Malassezia 종은 온혈 척추동물의 정상 미생물총을 구성하며, 개에서는 비지방친화성인 M. pachydermatis가 주로 감염을 유발하는 것으로 알려져 있다. 본 연구는 습도가 높은 한국의 여름 동안 개에 감염을 일으킨 말라세지아종의 분포를 조사하기 위해 실시되었다. 2006년 6월부터 10월까지 총 50개의 샘플을 채취하여 배양한 후 PCR-RFLP와 direct sequencing을 통해 분석하였다. 조사 결과 지방친화성 종인 M. furfur와 M. obtusa가 전체 96%를 차지하였으며, M. pachydermatis는 4%만을 구성하였다. 이 결과는 기후에 따라 감염을 일으키는 Malassezia 종에 차이가 있을 수 있음을 보여준다.

Keywords

References

  1. Cabanes FJ, Theelen B, Castella G, Boekhout T. Two new lipid-dependent Malassezia species from domestic animals. FEMS Yeast Res 2007; 7: 1064-1076. https://doi.org/10.1111/j.1567-1364.2007.00217.x
  2. Cafarchia C, Callo S, Capelli G, Otranto D. Occurrence and population size of Malassezia spp. in the external ear canal of dogs and cats both healthy and with otitis. Mycopathologia 2005; 160: 143-149. https://doi.org/10.1007/s11046-005-0151-x
  3. Cafarchia C, Gallo S, Romito D, Capelli G, Chermette R, Guillot J, Otranto D. Frequency, body distribution, and population size of Malassezia species in healthy dogs and in dogs with localized cutaneous lesions. J Vet Diagn Invest 2005; 17: 316-322. https://doi.org/10.1177/104063870501700403
  4. Chen TA, Hill PB. The biology of Malassezia organisms and their ability to induce immune responses and skin diseases. Vet Dermatol 2005; 16: 4-26. https://doi.org/10.1111/j.1365-3164.2005.00424.x
  5. Crespo MJ, Abarca ML, Cabanes FJ. Atypical lipiddependent Malassezia species isolated from dogs with otitis externa. J Clin Microbiol 2000; 38: 2383-2385.
  6. Crespo MJ, Abarca ML, Cabanes FJ. Occurrence of Malassezia spp. in the external ear canals of dogs and cats with and without otitis externa. Med Mycol 2002; 40: 115-121. https://doi.org/10.1080/mmy.40.2.115.121
  7. Crespo MJ, Abarca ML, Cabanes FJ. Otitis externa associated with Malassezia sympodialis in two cats. J Clin Microbiol 2000; 38: 1263-1266.
  8. Foster AP, Foil CS. BSAVA Manual of Small Animal Dermatology, 2nd ed. Quedgeley: British Small Animal Veterinary Association. 2003: 175-180.
  9. Guillot J, Breugnot C, Barros M, Chermette R. Usefulness of modified dixon's medium for quantitative culture of Malassezia species from canine skin. J Vet Diagn Invest 1998; 10: 384-386. https://doi.org/10.1177/104063879801000418
  10. Inamadar AC, Palit A. The genus Malassezia and human disease. Indian J Dermatol Venereol Leprol 2003; 69: 265-270.
  11. LjubojeviC S, Skerlev M, Lipozencic J, Basta-juzbasic A. The role of Malassezia furfur in dermatology. Clin Dermatol 2002; 20: 179-182. https://doi.org/10.1016/S0738-081X(01)00240-1
  12. Marcon MJ, Powell DA. Human infections due to Malassezia spp. Clin Microbiol Rev 1992; 5: 101-119. https://doi.org/10.1128/CMR.5.2.101
  13. Mirhendi H, Makimura K, Zomorodian K, Yamada T, Sugita T, Yamaguchi H. A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species. J Microbial Methods 2005; 61: 281-284. https://doi.org/10.1016/j.mimet.2004.11.016
  14. Nardoni S, Mancianti F, Corazza M, Rum A. Occurrence of Malassezia species in healthy and dermatologically diseased dogs. Mycopathologia 2004; 157: 383-388. https://doi.org/10.1023/B:MYCO.0000030416.36743.dd
  15. Porro MN, Passi S, Caprilli F, Nazzaro P, Morpurgo G. Growth requirements and lipid metabolism of Pityrosporum orbiculare. J Invest Dermatol 1976; 66: 178-182. https://doi.org/10.1111/1523-1747.ep12481919
  16. Roberts SO. Pityriasis versicolor: a clinical and mycological investigation. Br J Dermatol 1969; 81: 315-326. https://doi.org/10.1111/j.1365-2133.1969.tb13990.x
  17. Scott DW, Miller WH, Griffin CE. Fungal skin disease. In: Muller and Kirk's Small Animal Dermatology, 6th ed. Philadelphia: WB Sounders. 2001: 336-422.