Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Acute Toxicity Study on Cheongsimyeonja-tang in Mice

Mouse에 있어서 시험물질 청심연자탕(淸心蓮子湯) 추출액의 급성독성 시험

  • Ma, Jin-Yeul (TKM Converging Research Division, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Si-Woo (Constitutional Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Jong-Yeol (Constitutional Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Standard Research Division, Korea Institute of Oriental Medicine)
  • 마진열 (한국한의학연구원 한의융합연구본부) ;
  • 황대선 (한국한의학연구원 표준화연구본부) ;
  • 서창섭 (한국한의학연구원 표준화연구본부) ;
  • 이시우 (한국한의학연구원 체질의학연구본부) ;
  • 김종열 (한국한의학연구원 체질의학연구본부) ;
  • 신현규 (한국한의학연구원 표준화연구본부)
  • Received : 2009.10.22
  • Accepted : 2009.12.02
  • Published : 2010.03.31
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Abstract

1. Objectives: The aim of this study is data analysis for acute toxicity and safety of Cheongsimyeonja-tang. 2. Methods: We investigated the acute toxicity for water-extracted Cheongsimyeonja-tang. Fifty five male and female mice were observed for 14 days after one day oral administration of Cheongsimyeonja-tang at the respective doses of 0 (control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results: We observed survival rates, general toxicity, change of body weight and autopsy. In animals administered with Cheongsimyeonja-tang, there were nither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign and autopsy. 4. Conclusion: The data confirmed that Cheongsimyeonja-tang is free from the toxicity and safety problems in treated groups. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). Lethal Dose 50 (LD50) value for mice was more than 5000 mg/kg per oral for both male and females. It suggest that Cheongsimyeonja-tang in mice is considered to be safe.

Keywords

References

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