Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Effect of Chongmyung-Tang Prescription Combination on the Production of Amyloid β protein and β-site amyloid precursor protein-cleaving enzyme Activity in vitro

In vitro에서 β-site amyloid precursor protein-cleaving enzyme 활성과 amyloid β protein 생산에 대한 총명탕가미방(聰明湯加味方)의 효과

  • Lim, Jung-Hwa (Dept. of Neuropsychiatry, Pu-San National University Korean Medicine Hospital) ;
  • Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University) ;
  • Lim, Jong-Soon (Dept. of Life Science and Preventative Medicine College of Oriental Medicine, Dae-Jeon University) ;
  • Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, Dae-Jeon University) ;
  • Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University)
  • 임정화 (부산대학교병원 한방진료처 한방신경정신과) ;
  • 정인철 (대전대학교 한의과대학 신경정신과학교실) ;
  • 임종순 (대전대학교 한의과대학 생명예방과학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 이상룡 (대전대학교 한의과대학 신경정신과학교실)
  • Received : 2010.05.08
  • Accepted : 2010.06.03
  • Published : 2010.06.30
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Abstract

Objectives : This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-$C_{1-10}$) extract on the production of amyloid $\beta$ protein and $\beta$-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods : The effect of CmTP-$C_{1-10}$ extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid $\beta$ protein fragment(A$\beta$ fragment) were investigated. The effect of CmTP-$C_{1-10}$ extract on production of amyloid $\beta$ protein(A$\beta$) in BV2 microglia cell line treated by LPS and A$\beta$ fragment were investigated. The effect of CmTP-$C_{1-10}$ extract on BACE activity were investigated. Results : 1. CmTP-$C_9$ extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 2. CmTP-$C_9$ extract significantly suppressed the production of A$\beta$ in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 3. CmTP-$C_9$ extract the most significantly inhibited BACE activity. Conclusions : These results suggest that CmTP-$C_9$ may be effective for the prevention and treatment of Alzheimer's Disease. Investigation into clinical use of CmTP-$C_9$ for Alzheimer's Disease is suggested for future research.

Keywords

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