생명과학회지 (Journal of Life Science)

  • 제20권5호
  • /
  • Pages.676-682
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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하수처리수에서 분리된 장내세균의 광범위 베타락탐분해효소의 유형

Types of Extended-Spectrum β-Lactamase Produced in Enteric Bacteria Isolated from Sewage Plant Drain Water

  • 김군도 (부경대학교 자연과학대학 미생물학과) ;
  • 이훈구 (부경대학교 자연과학대학 미생물학과)
  • Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Lee, Hun-Ku (Department of Microbiology, College of Natural Sciences, Pukyong National University)
  • 투고 : 2010.04.06
  • 심사 : 2010.05.25
  • 발행 : 2010.05.31
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초록

본 연구는 임상검체만이 아닌 주변 생활환경에도 광범위 베타 락탐분해효소를 생성하는 균주(extended-spectrum $\beta$-lactamase, ESBL)가 존재하는지를 확인하고 만약 존재할 경우 그 균주를 분리하고 ESBL유형을 알아보기 위하여 실시되었다. 부산 광안리 하수처리 방류수에서 이중 디스크 확산 검사 결과 양성반응을 나타낸 29균주를 선별하였다. 이중 sodium azide에 내성을 가진 피전달 균주인 Escherichia coli J5에 교차접합이 이루어진 15균주를 대상으로 indole, methyl-red, Voges-Proskauer, Simmon's citrate 시험과 decarboxylase-dihydrolase 및 여러 종류의 당 발효 시험 등 생화학 검사를 실시한 결과 Klebsiella pneumoniae(13균주)와 Escherichia coli(2균주)가 동정되었다. 등전점, PCR, 유전자서열 분석을 실시하여 ESBL 유형을 결정하였다. Klebsiella pneumoniae의 ESBL 생성유형은 SHV-12(4균주)와 SHV-12/TEM-1(9균주)의 2종류로 구분되었고, Escherichia coli의 ESBL 생성유형은 TEM-1(2균주)로 판명되었다.

This study focused on typing of the extended-spectrum $\beta$-lactamase (ESBL) produced in organisms isolated from a natural environment, rather than a clinical setting. Samples were collected from drain water issuing from a sewage plant in Kwanganri (Busan, Korea). Following double disk synergy testing, 29 strains were selected as potential ESBL positive strains. Of these, 15 strains were transconjugants of the sodium azide resistant recipient strain Escherichia coli J53 and analyzed biochemically including indole, methyl-red, Voges-Proskauer, Simmon's citrate, decarboxylase-dihydrolase and sugar-fermentation tests. The tests classified the 15 strains as Klebsiella pneumoniae (n=13) and Escherichia coli (n=2). The type of ESBL from each strain was deduced by isoelectric focusing point analysis and DNA sequencing. The results indicated that the types of ESBL were SHV-12 (n=4) and SHV-12/TEM-1 (n=9) from K. pneumoniae and TEM-1 (n=2) from E. coli strains.

키워드

참고문헌

  1. Ambuler, R. P., A. F. W. Coulson, N. M. Frere, J. M. Ghuysen, B. Joris, M. Forsman, R. C. Lebversque, G. Tiraby, and S. G. Waley. 1991. A standard numbering scheme for class A $\beta$-lactamases. Biochem. J. 276, 269-272.
  2. Brinas, L., M. Zarazaga, Y. Saenz, F. Ruiz-Larrea, and C. Torres. 2002. $\beta$-lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals. Antimicrob. Agents Chemother. 46, 3156-3163. https://doi.org/10.1128/AAC.46.10.3156-3163.2002
  3. Bush, K. 1989. Characterization of $\beta$-lactamases. Antimicrob. Agents Chemother. 33, 259-263. https://doi.org/10.1128/AAC.33.3.259
  4. Bush, K. and G. Jacoby. 1997. Nomenclature of TEM $\beta$-lactamases. Antimicrob. Chemother. 39, 1-3.
  5. Bush, K., G. A. Jacoby, and A. A. Medeiros. 1995. A functional classification scheme for $\beta$-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39, 1211-1233. https://doi.org/10.1128/AAC.39.6.1211
  6. Ewing, W. H. 1986. The genus Klebsiella. In W.H. Ewing, Edwards and Ewing's Identification of Enterobacteriaceae. Elsevier, N.Y.
  7. Grimont, F., P. A. D. Grimont, and C. Richard. 1991. The genus Klebsiella, p. 2775-2796. The prokaryotes Vol. III, 2nd ed. Springer-Verlag. N. Y.
  8. Jacoby, G. A. 1994. Genetics of extended-spectrum beta- lactamases. Eur. J. Clin. Microbiol. Infect. Dis. 13, 2-11. https://doi.org/10.1007/BF02390679
  9. Jacoby, G. A. and I. Carreras. 1990. Activities of $\beta$-lactam antibiotics against Escherichia coli strains producing extended- spectrum $\beta$-lactamases. Antimicrob. Agents Chemother. 34, 859-862.
  10. Jeong, Y. S., J. C. Lee, H. Y. Kang, H. S. Yu, E. Y. Lee, C. H. Choi, S. H. Tae, Y. C. Lee, D. T. Cho, and S. Y. Seol. 2003. Epidemiology of nalidixic acid resisitance and TEM-1-and TEM-52-mediated ampicillin resistance of Shigella sonnei isolates obtained in Korea between 1980 and 2000. Antimicrob. Agents Chemother. 47, 3719-3723. https://doi.org/10.1128/AAC.47.12.3719-3723.2003
  11. Kim, J., Y. Kwon, H. Pai, J. W. Kim, and D. T. Cho. 1998. Survey of Klebsiella pneumoniae strains producing extended-spectrum $\beta$-lactamase: prevalence of SHV- 12 and SHV-2a in Korea. J. Clin. Microbiol. 36, 1446-1449.
  12. Kim, Y. T., and H. K. Lee. 2000. Extended-spectrum $\beta$- lactamase (ESBL) typing of Klebsiella pneumoniaee isolated from clinical specimen in Pusan. Kor. J. Microbiol. 36, 221-227.
  13. Kirby, R. 1992. Evolutionary origin of the class A and class C $\beta$-lactamases. J. Mol. Evol. 34, 345-350. https://doi.org/10.1007/BF00160242
  14. Lee, H. K, 2006. Typing of extended-spectrum $\beta$-lactamase (ESBL) producing Enterobacteriaceae isolated from slaughter house in Pusan, Korea. Kor. J. Microbiol. 42, 125-130.
  15. Lee, H. K., H. J. Kim, and G. D. Kim. 2007. Typing of extended- spectrum $\beta$-lactamases of Escherichia coli and Klebsiella pneumoniae isolated from rivers in Pusan, Korea. Kor. J. Microbiol. 43, 116-123.
  16. Matthew, M., A. M. Harris, M. J. Marshall, and G. W. Ross. 1975. The use of analytical isoelectric focusing for detection and identification of $\beta$-lactamases. Gen. Microbiol. 88, 169-178. https://doi.org/10.1099/00221287-88-1-169
  17. Medeiros, A. A. 1993. Nosocomial outbreaks of multiresistant bacteria: extended - spectrum beta-lactamases have arrived in North America. Ann. Intern. Med. 119, 428-430. https://doi.org/10.7326/0003-4819-119-5-199309010-00015
  18. National Committee for Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests. NCCLS document M2-A6. NCCLS, Wayne. PA.
  19. Nueesch-Inderbinen, M. T., F. H. Kayser, and H. Haechlier. 1997. Survey and molecular genetics of SHV $\beta$-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12. Antimicrob. Agents Chemother. 41, 943-949.
  20. Pai, H. J, S. Lyu, J. H. Lee, J. Kim, and Y. Kwon. 1999. Survey of extended- spectrum $\beta$-lactamase in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea. J. Clin. Microbiol. 37, 1758-1763.
  21. Philippon, A., R. Labia, and G. Jacobi. 1989. Extended-spectrum $\beta$-lactamases (minireview). Antimicrob. Agents Chemother. 33, 1131-1136. https://doi.org/10.1128/AAC.33.8.1131
  22. Sambrook, J., F. E. Fritsch, and T. Maniatis. 1989. Molecular cloning laboratory manual, Vol. I. 1.25-1.28, Cold Spring Harbor Laboratory Press.
  23. Silva, J., R. Gatoca, C. Auguilar, Z. Becerra, U. Garza- Ramos, M. Velazqez, G. Miranda, B. Leanos, F. Solorzano, and G. Echaniz. 2001. Outbreak of infection with extended-spectrum $\beta$-lactamase producing Klebsiella pneumoniae in a Mexican hospital. J. Clin. Microbiol. 39, 3193-3196 https://doi.org/10.1128/JCM.39.9.3193-3196.2001
  24. Teshager, T., L. Dominguez, M. A. Moreno, Y. Saenz, C. Torres, and S. Cardenosa. 2000. Isolation of an SHV-12 $\beta$-lactamase-producing Escherichia coli strain from a dog with recurrent urinary tract infections. Antimicrob. Agents Chemother. 44, 3483-3484. https://doi.org/10.1128/AAC.44.12.3483-3484.2000