Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

RAPD Analysis and Antimicrobial Susceptibility of Streptococcus equi subsp. zooepidemicus Isolated from Thoroughbred Horses

더러브렛 말에서 분리한 Streptococcus equi subsp. zooepidemicus의 RAPD 분석 및 약제 감수성

  • Received : 2010.02.04
  • Accepted : 2010.04.27
  • Published : 2010.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

A total of 68 samples were collected including vaginal mucosa (n=66) from Jangsu stud farm, an equine aborted fetus (n=1), and uterine contents (n=1) from Jeju island. Seventeen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) strains isolated from horses in Korea were identified as S. zooepidemicus by biochemical tests and sodA.seeI specific multiplex PCR. All isolated strains were divided into 4 clusters: group 1 (No. 2, 3, 5, 6, 7, 11, 12, 13, 14, 15), group 2 (No. 4, 9), group 3 (No. 10, 16, 17), and group 4 (No. 1, 8) by RAPD typing. In group 3, No. 10 isolate that was isolated from vaginal mucosa was indistinguishable from No. 16 and 17 isolates, which were isolated from the equine uterine contents and the equine aborted fetus, respectively. The results of this study suggest that a limited epidemiological relationship exists between the strains from Jangsu (No. 10) and Jeju (No. 16 and No. 17). All isolates showed a high susceptibility to ampicillin, cefoxitin, ceftiofur, cephalothin, florofenicol, gentamicin, nalidixic acid, oxacillin, penicillin, tiamulin, tylosin and vancomycin in antimicrobial susceptibility tests. These results may provide the basic information needed to establish strategies for the treatment and prevention of reproductive diseases in mares in Korea.

Streptococcus equi subsp. zooepidemicus는 말의 생식기 질환을 유발하는 중요한 병원체 중 하나로서 국내 말의 생산성 향상을 도모할 목적으로 제주도에서 사육중인 더러브렛 암말의 유산태아 및 그 말의 자궁내용물과 장수목장에서 암말의 교배전 생식기 질환이 의심되는 말의 질 내용물로부터 시료를 채취하여 Streptococcus equi subsp. zooepidemicus을 분리하여 그 분리균의 특성 및 항균제 감수성 검사를 실시한 결과, RAPD typing에서는 크게 4개의 cluster로 구분되었다. 특히 제주도에서 분리한 균주와 장수목장에서 분리한 하나의 균주가 동일한 pattern을 나타내었다. 항균제 감수성 검사에서는 대부분의 균주가 ampicillin 등의 약제에 감수성이 있음을 확인하였다. 본 연구의 결과는 국내에서 사육중인 암말의 생식기 질병의 예방 및 치료에 유용할 것으로 생각된다.

Keywords

References

  1. Abdulmawjood, A. and C. H. Lammler. 2000. Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus. Res. Vet. Sci. 68, 33-39. https://doi.org/10.1053/rvsc.1999.0332
  2. Alber, J., A. EI-Sayed, C. Lammler, A. Hassan, R. Weiss, and M. Zschock. 2004. Multiplex polymerase chain reaction for identification and differentiation of Streptococcus equi subsp. zooepidemicus and Streptococcus equi subsp. equi. J. Vet. Med. 51, 455-458. https://doi.org/10.1111/j.1439-0450.2004.00799.x
  3. Allpress, R. G. 1997. The treatment of lower airway disease in a group of young Thoroughbreds in training. Equine Vet. Ed. 9, 68-71. https://doi.org/10.1111/j.2042-3292.1997.tb01280.x
  4. Anzai, T., T. A. Walker, M. B. Blair, T. M. Chambers, and J. F. Timoney. 2000. Comparison of the phenotypes of Streptococcus zooepidemicus isolated from tonsils of healthy horses and specimens obtained from foals and donkeys with pneumonia. Am. J. Vet. Res. 61, 162-166. https://doi.org/10.2460/ajvr.2000.61.162
  5. Anzai, T., J. F. Timoney, T. Kuwamoto, R. Wada, M. Oikawa, and T. Higuchi. 2002. Polymerase chain reaction restriction fragment length polymorphism analysis of the SzP gene of Streptococcus zooepidemicus isolated from the respiratory tract of horses. Am. J. Vet. Res. 63, 1298-1301. https://doi.org/10.2460/ajvr.2002.63.1298
  6. Balter, S., A. Benin, S. W. Pinto, L. M. Teixeira, G. G. Alvim, D. Luna Jackson, L. Laclaire, J. Elliot, R. Facklam, and A. Schuchat. 2000. Epidemic nephritis in Nova Serrana, Brazil. Lancet. 355, 1776-1780. https://doi.org/10.1016/S0140-6736(00)02265-0
  7. Barnham, M., T. J. Thornton, K. Lange. 1983. Nephritis caused by Streptococcus zooepidemicus (Lancefield group C). Lancet. 1, 945-948.
  8. Bauer, A. W., W. M. M. Kirby, and J. C. Sherris. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493-496.
  9. Bert, F., B. Picard, C. Branger, and N. Lambert Zechovsky. 1996. Analysis of genetic relationships among strains of group A, C and G streptococci by random amplified polymorphic DNA analysis. J. Med. Microbiol. 45, 278-284. https://doi.org/10.1099/00222615-45-4-278
  10. Bordes-Benitez, A., M. Sanchez-Onoro, P. Suarez-Bordon, A. J. Garcia-Rojas, J. A. Saez-Nieto, A. Gonzalez-Garcia, I. Alamo-Antunez, A. Sanchez-Maroto, and M. Bolanos-Rivero. 2006. Outbreak of Streptococcus equi subsp. zooepidemicus infections on the island of Gran Canaria associated with the consumption of inadequately pasteurized cheese. Eur. J. Clin. Microbiol. Infec. Dis. 25, 242-246. https://doi.org/10.1007/s10096-006-0119-x
  11. Chalker, V. J., H. W. Brooks, and J. Brownlie. 2003. The association of Streptococcus equi subsp. zooepidemicus with canine infections respiratory disease. Vet. Microbiol. 95, 149-156. https://doi.org/10.1016/S0378-1135(03)00155-X
  12. Choi, S. K., S. G. Lee, J. H. Yang, and G. J. Cho. 2007. Distribution and antimicrobial susceptibility patterns of bacteria isolated from genital tract in Thoroughbred mares. J. Vet. Clin. 24, 19-25.
  13. Cowan, S. T. and K. J. Steel. 1993. Cowan and Steel's Manual for the Identification of Medical Bacteria, third ed. Cambridge University Press, Cambridge.
  14. Dawlat, A., R. Orman, I. Essam, and M. Ahmed. 2002. Abortion and stillbirth in Arabian mare: clinical, etiological and pathological studies in Arabian mare. Assiut. Vet. Med. J. 47, 279-295.
  15. Erdeger, J., M. Akan, A. Dakman, G. Altay, R. Vural, and M. Celebi. 1999. Kisraklarin genital organlarrindan izole edilen $\beta$-hemolitik streptokok’larin identifikasyonu ve serogruplanirilmasi. Ankara. Univ. Vet. Fak. Derg. 46, 69-76.
  16. Giles, R. C., J. M. Donahue, C. B. Hong, P. A. Tuttle, M. B. Petrites-Murphy, K. B. Poonacha, A. W. Roberts, R. R. Tramontin, B. Smith, and T. W. Swerczek. 1993. Causes of abortion, stillbirth, and perinatal death in horses: 3527 cases (1986-1991). J. Am. Vet. Med. Assoc. 203, 1170-1175.
  17. Hashikawa, S., Y. Iinuma, M. Furushita, T. Ohkura, T. Nada, K. Torii, T. Hasegawa, and M. Ohta. 2004. Characterization of group C and G streptococcal strains that cause streptococcal toxic shock syndrome. J. Clin. Microbiol. 42, 186-192. https://doi.org/10.1128/JCM.42.1.186-192.2004
  18. Hong, C. B., J. M. Donahue, R. C. J. Giles, M. B. Petrites- Murphy, K. B. Poonacha, A. W. Roberts, B. J. Smith, R. R. Tramontin, P. A. Tuttle, and T. W. Swerczek. 1993. Equine abortion and stillbirth in central Kentucky during 1988 and 1989 foaling seasons. J. Vet. Diagn. Invest. 5, 560-566. https://doi.org/10.1177/104063879300500410
  19. Julian, A. F. 1992.Equine abortions in New Zealand between 1974 and 1990. Surveill Wellington. 19, 24-25.
  20. Kocabiyik, A. L., G. Sonmez, M. Ulgen, C. Ozakin, E. Kocakaya, and A. Alasonyalilar. 2005. Abortion due to Streptococcus equi subsp. zooepidemicus in a mare. Turk. J. Vet. Anim. Sci. 29, 937-940.
  21. Kuusi, M., E. Lahti, A. Virolainen, M. Hatakka, R. Vuento, L. Rantala, J. Vuopio-Varkila, E. Seuna, M. Karppelin, M. Hakkinen, J. Takkinen, V. Gindonis, K. Siponen, and K. Huotari. 2006. An ourbreak of Streptococcus equi subsp. zooepidemicus associated with consumption of fresh goat cheese. BMC. Infect. Dis. 6, 36-41. https://doi.org/10.1186/1471-2334-6-36
  22. Lannergard, J., L. Frykberg, and B. Guss. 2003. CNE, a collagen- binding protein of Streptococcus equi. FEMS. Microbiol. Lett. 222, 69-74. https://doi.org/10.1016/S0378-1097(03)00222-2
  23. Las Heras, A., A. I. Vela, E. Fernandez, E. Legaz, L. Dominguez, and J. F. Fernandez-Garayzabal. 2002. Unusual outbreak of clinical mastitis in dairy sheep caused by Streptococcus equi subsp. zooepidemicus. J. Clin. Microbiol. 40, 1106-1108. https://doi.org/10.1128/JCM.40.3.1106-1108.2002
  24. Luque, I., J. F. Fernandez-Garayzabal, V. Blume, A. Maldonado, R. Astorga, and C. Tarradas. 2006. Molecular typing and anti-microbial susceptibility of clinical isolates of Streptococcus equi spp. zooepidemicus from equine bacterial endometritis. J. Vet. Med. B. 53, 451-454. https://doi.org/10.1111/j.1439-0450.2006.01001.x
  25. Pisoni, G., R. N. Zadoks, C. Vimercati, C. Locatelli, M. G. Zanoni, and P. Moroni. 2009. Epidemiological investigation of Streptococcus equi subspecies zooepidemicus involved in clinical mastitis I dairy goats. J. Dairy. Sci. 92, 943-951. https://doi.org/10.3168/jds.2008-1548
  26. Smith, K. C., A. S. Blunden, K. E. Whitwell, K. A. Dunn, and A. D. Wales. 2003. A survey of equine abortion, stillbirth and neonatal death in the UK from 1988 to 1997. Equine Vet. J. 35, 496-501. https://doi.org/10.2746/042516403775600578
  27. Soedarmanto, I., F. H. Pasaribu, I. W. T. Wibawan, and C. Lammler. 1996. Identification and molecular characterization of serological group C streptococci isolated from diseased pigs and monkeys in Indonesia. J. Clin. Microbiol. 34, 2201-2204.
  28. Timoney, J. F. 2004. The pathogenic equine streptococci. Vet. Res. 35, 397-409. https://doi.org/10.1051/vetres:2004025
  29. Younan, M., A. T. S. Estoepangestie, M. Gengiz, J. Alber, A. El-Sayed, and C. Lammler. 2005. Identification and Molecular Characterization of Streptococcus equi subsp. zooepidemicus Isolated from Camels (Camelus dromedaries) and Camel Milk in Kenya and Somalia. J. Vet. Med. B. 52, 142-146. https://doi.org/10.1111/j.1439-0450.2005.00828.x
  30. Van Belkum, A. 1994. DNA fingerprinting of medically important microorganisms by use of PCR. Clin. Microbiol. Rev. 7, 174-184.
  31. Vogel, L., G. Jones, S. Triep, A. Koek, and L. Dijkshoorn. 1999. RAPD typing of Kelbsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens and Pseudomonas aeruginosa isolates using strandardized reagents. Clin. Microbiol. Infect. 5, 270-276. https://doi.org/10.1111/j.1469-0691.1999.tb00140.x
  32. Walker, J. A. and J. F. Timoney. 1997. Molecular basis of variation in protective SzP proteins of Streptococcus zooepidemicus. Ad. Exp. Med. Biol. 418, 611-613. https://doi.org/10.1007/978-1-4899-1825-3_142
  33. Yoshikawa, H., T. Yasu, H. Ueki, T. Oyamada, H. Oishi, T. Anzai, M. Oikawa, and T. Yoshikawa. 2003. Pneumonia in horses induced by intrapulmonary inoculation of Streptococcus equi subsp. zooepidemicus. J. Vet. Med. Sci. 65, 787-792. https://doi.org/10.1292/jvms.65.787

Cited by

  1. A case of meningitis caused by Streptococcus equi subsp. zooepidemicus infection in a foal in Jeju vol.37, pp.1, 2014, https://doi.org/10.7853/kjvs.2014.37.1.67
  2. Biochemical characteristics and antimicrobials susceptibility of pathogenic Streptoccus spp. isolated from respiratory tract of Thoroughbred horses in Jeju, Korea vol.34, pp.3, 2011, https://doi.org/10.7853/kjvs.2011.34.3.209