Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Improvement of Antibacterial Activities of Bacteriocidal Yeasts Using the GPD Promoter

GPD 프로모터를 이용한 항균활성 효모의 활성증진

  • Jang, Min-Kyung (Department of Pharmaceutical Engineering, Silla University) ;
  • Yu, Ki-Hwan (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Nam-Young (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Ok-Hee (Department of Pharmaceutical Engineering, Silla University) ;
  • Shin, Jae-Kyun (Department of Pharmaceutical Engineering, Silla University) ;
  • Jang, Hye-Ji (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Seung-Woo (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
  • Received : 2010.04.07
  • Accepted : 2010.04.13
  • Published : 2010.06.30
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Abstract

We have previously reported recombinant productions of bacteriocins using yeast expression plasmid pAUR123, which contains the alcohol dehydrogenase (ADH) promoter, in Saccharomyces cerevisiae cells and their antibacterial activities. In order to improve the antibacterial activities of bacteriocidal yeast cells, a strong glyceraldehyde phosphate dehydrogenase (GPD) promoter gene of S. cerevisiae was amplified and inserted upstream into bacteriocin genes such as the OR-7, Subpeptin JM4-A or JM4-B gene in the corresponding recombinant yeast plasmid. Yeast cells transformed by the recombinant plasmid containing the GPD promoter represented higher antibacterial activities against both Gram positive B. subtilis and Gram negative E. coli cells compared to those transformed by the corresponding recombinant plasmid containing the ADH promoter. Thus, yeast cells harboring the recombinant plasmid containing the GPD promoter constructed in this study could be applied in the food preservative or animal feed industries.

박테리오신의 항균활성 개선을 위해 선행연구에서 개발된 ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 효모발현 벡터에 GPD 프로모터 단편을 도입하여 강력한 프로모터를 가진 효모발현 재조합 플라스미드DNA를 제작하였다. ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 기존의 형질전환 효모들에 비해 새롭게 개발된 GPD 프로모터에 의해 박테리오신 유전자를 발현시키는 형질전환 효모들이 그람양성 대표세균인 고초균(B. subtilis)과 그람음성 장내세균인 대장균(E. coli) 모두에서 보다 높은 생육억제환을 나타내는 것을 확인 하였다. 이 연구의 결과로 GPD 프로모터에 의해 발현이 유도되는 박테리오신 생산 효모들을 이용하여 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질의 산업적인 생산이 가능하게 될 것으로 기대된다.

Keywords

References

  1. Antonio, G., H. Abriouel, R. L. Lopez, and N. B. Omar. 2007. Bacteriocin-based strategies for food biopreservation. Int. J. Food Microbiol. 120, 51-70. https://doi.org/10.1016/j.ijfoodmicro.2007.06.001
  2. Avis, T. J., R. Anguenot, B. Neveu, S. Bolduc, Y. Zhao, Y. Cheng, C. Labbe, and F. Belanger. 2008. Usefulness of heterologous promoters in the Pseudozyma flocculosa gene expression system. Biosci. Biotechnol. Biochem. 72, 456–462.
  3. Chun, B. W., D. H Kim, B. W. Chung, H. C. Lee, and M. S. Yang. 1996. Production of glucose oxidase using recombinant yeast. Kor. J. Biotechnol. Bieong. 31, 207-275.
  4. Dixon, B. 2000. Antibiotics as growth promoters: risks and alternatives. ASM news. 66, 264-265.
  5. Flynn, S., D. van Sinderen, G. M. Thornton, H. Holo, F. Nes, and J. K. Collins. 2002. Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Mircrobiology 148, 973-984.
  6. Friedman, C. R., J. Neiemann, H. C. Wegener, and R. V. Tauxe. 2000. Epidemiology of Campylobacter jejuni infections in the Unites States and other industrialized nations, pp. 121-138, In Nachamkin, I. and M. J. Blaser (eds.), Campylobacter, 2nd eds. American Society for Microbiology, Washing D.C.
  7. Fuller, R. 1989. Probiotics in man and animal. Bacteriology 66, 265-378.
  8. Gong, Z., Y. Su, L. Huang, J. Lin, K. Tang, and X. Zhou. 2009. Cloning and analysis of glyceraldehyde-3-phosphate dehydrogenase gene from Cordyceps militaris. Afr. J. Agric. Res. 4, 402-408.
  9. Kang, H. K., H. J. Chun, and M. W. Lee. 2000. The optimization of recombinant protein production using S. cerevisiae mutant Y334 suitable for Gal promoter. Kor. J. Biotechnol. Bioeng. 2, 181-187.
  10. Kim, H. Y., Y. J. Lee, K. H. Hong, Y. K. Kwon, J. Y. Lee, and S. H. Kim. 1999. Studies on the development of natural preservatives from natural products. Kor. J. Food Sci. Technol. 31, 1667-1678.
  11. Lee, O. H., M. K. Jang, D. G. Lee, I. H. Kim, J. H. Lee, J. M. Ha, B. J. Ha, I. Y. Ahn, D. I. Cho, and S. H. Lee. 2008. Establishment of an antibacterial yeast that producing bacteriocin Subpeptin JM4-A or Subpeptin JM4-B. J. Life Sci. 18, 287-290. https://doi.org/10.5352/JLS.2008.18.2.287
  12. Lee, O. H., M. K. Jang, D. G. Lee, J. H. Lee, J. M. Ha, B. J. Ha, I. Y. Ahn, D. I. Cho, and S. H. Lee. 2008. Construction of a bacteriocidal yeast producing bactericin OR-7. Kor. J. Microbiol. Biotechnol. 36, 101-105.
  13. Lim, Y. Y., E. H. Park, J. H. Kim, S. M. Park, H. S. Jang, Y. J. Park, A. Y. Yang, M. S. Yang, and D. H. Kim. 2001. Enhanced and targeted expression of fungal phytase in Saccharomyces cerevisiae. Kor. J. Microbiol. Biotechnol. 11, 915-921.
  14. Park, J. H., N. S. Han, J. Y. Yoo, D. J. Kwon, H. K. Shin, and Y. J. Koo. 1993. Screening of the foodstuffs influencing the growth of Bifidobacterium spp. and Clostridum perfringens. Kor. J. Food Sci. Technol. 25, 582-588.
  15. Seo, H. Y., E. S. Jeon, Y. J. Chung, and S. K. Kim. 2002. Heterologous expression of human ferritin H-chain and L-chain genes in Saccharomyces cerevisiae. Kor. J. Biotechnol. Bioeng. 2, 162-168.
  16. Varma, A. and K. J. Kwon-Chung. 1999. Characterization of the glyceraldehyde-3-phosphate gene and the use of its promoter for heterologous expression in Cryptococcus neoformans, a human pathogen. Gene 232, 155-163. https://doi.org/10.1016/S0378-1119(99)00132-8
  17. Wolff, A. M. and J. Arnau. 2002. Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpdI promoter for recombinant protein production. Fungal Genet. Biol. 35, 21-29. https://doi.org/10.1006/fgbi.2001.1313
  18. Young, K. D., M. Koo, and C. R. Ryoo. 2002. Bacteriocin produced by Pediococcus sp. in Kimchi and its characteristics. Kor. J. Microbiol. Biotechnol. 12, 96-105.