Journal of Industrial Technology (산업기술연구)
- Volume 30 Issue B
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- Pages.61-68
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- 2010
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- 1229-9588(pISSN)
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- 1598-1371(eISSN)
Cloning of Serratia marcescens KFRI314 chitinase genes and its role on chitin degradation
Serratia marcescens KFRI314 chitinase 유전자의 클로닝과 키틴분해에 관한 효소의 역할
Abstract
Three chitinase genes (chiA, chiB, and chiC) were cloned into E. coli by PCR amplification from Serratia marcescens KFRI314. The sizes of cloned chitinase genes were 1692 bp, 1500 bp, and 1443 bp which correspond to 563, 499, and 480 amino acids, respectively. Recombinant chitinases were overexpressed using pHCEIA expression vector and purified to homogenity. The molecular weights of chitinases were about 60kDa, 50 kDa, 52 kDa, respectively. Optimum pHs were around pH 5~6 and optimum temperatures were