Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Evaluation of the Antioxidant Properties of Pediastrum duplex and Dactylococcopsis fascicularis Microalgae

  • Lee, Seung-Hong (Department of Marine life Science, Jeju National University) ;
  • Lee, Won-Woo (Department of Marine life Science, Jeju National University) ;
  • Lee, Joon-Baek (Department of Earth and Marine Sciences, Jeju National University) ;
  • Jeon, You-Jin (Marine and Environmental Research Institute, Jeju National University)
  • Received : 2009.12.17
  • Accepted : 2010.03.15
  • Published : 2010.03.31
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Abstract

Interest in plant-derived food additives has increased recently, with efforts to identify natural antioxidant sources to replace synthetic antioxidants. This study evaluated the antioxidant effects of organic solvent fractions of 80% methanol extracts from Pediastrum duplex and Dactylococcopsis fascicularis microalgae. Among the solvent fractions tested, the ethyl acetate and n-hexane fractions from P duplex effectively scavenged 79.8% and 74.5% of DPPH free radicals, respectively. The chloroform fraction from D. fascicularis showed the strongest $H_2O_2$ scavenging ability (49.7%). The greatest scavenging of hydroxyl radicals (73.1%) was exhibited by the 80% methanol extract from P duplex. Aqueous residue and ethyl acetate fraction from P duplex provided the strongest nitric oxide scavenging (57.7%) and metal chelating effect (82.1%), respectively. Chloroform and ethyl acetate fractions from P duplex and n-hexane fraction from D. fascicularis exhibited significantly greater inhibition of lipid peroxidation than the commercial antioxidants. These results suggested that P duplex and D. fascicularis microalgae having potential anti oxidative compounds with various properties could be utilized in the food and pharmaceutical industries.

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References

  1. AOAC. 1980. Official Method of Analysis. 14th ed. Association of Official Analytical Chemists, Washington DC. P. 31.
  2. Benedettia S, Benvenutia F, Pagliarania S, Francoglia S, Scogliob S and Canestraria F. 2004. Antioxidant properties of a novel phycocyanin extract from the blue-green alga Aphanizomenon flos-aquae. Life Sci 75, 2353-2362. https://doi.org/10.1016/j.lfs.2004.06.004
  3. Brand-Williams W, Cuvelier ME and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol 28, 25-30.
  4. Chandler SF and Dodds JH, 1993. The effect of phosphate, nitrogen, and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rep 2, 105-110.
  5. Chung SK, Osawa T and Kawakishi S. 1997. Hydroxyl radical-scavenging effects of spices and scavengers from black mustard (Brassica nigra). Biosci Biotechnol Biochem 6, 118-123.
  6. Decker EA and Welch B. 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem 38, 674-677. https://doi.org/10.1021/jf00093a019
  7. Fridovich I. 1995. Superoxide radical and superoxide dismutases. Annu Rev Biochem 64, 97-112. https://doi.org/10.1146/annurev.bi.64.070195.000525
  8. Garrat DC. 1964. The Quantitative Analysis of Drugs. Chapman and Hall, Tokyo, Japan, 456-458.
  9. Gulcin I, Buyukokuroglu ME, Oktay M and Kufrevioglu OI. 2003. Antioxidant and analgesic activities of turpentine of Pinus nigra Am. subsp. pallsiana (Lamb.) Holmboe. J Ethnopharmacol 86, 51-58. https://doi.org/10.1016/S0378-8741(03)00036-9
  10. Gulcin I, Oktay M, Kufreviocglu O and Aslan A. 2002. Determination of antioxidant activity of lichen Cetraria islandica (L). Ach J Ethnopharmacol 79, 325-329. https://doi.org/10.1016/S0378-8741(01)00396-8
  11. Gulcin I. Beydemir S, Ahmet HA, Elmasta M and Buyukokuroglu ME. 2004. In vitro antioxidant properties of morphine. Pharmacol Res 49, 59-66. https://doi.org/10.1016/j.phrs.2003.07.012
  12. Halliwell B. 1991. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. Ame J Med 91, S14-S22.
  13. Halliwell Band Gutteridge JMC. 1989. Free radical in biology and medicine. Clarendon Press, Oxford, UK., 23-30.
  14. Hirata T, Tanaka M, Ooike M, Tsunomura T and Sakaguchi M. 2000. Antioxidant activities of phycocyanobilin prepared from Spirulina platensis. J Appl Phycol 12, 435-439. https://doi.org/10.1023/A:1008175217194
  15. Karawita R, Senevirathne M, Athukorala Y, Affan A, Lee YJ, Kim SK, Lee JB and Jeon YJ. 2007. Protective effect of enzymatic extracts from microalgae against DNA damage induced by $H_2O_2$. Mar Biotech 9, 479-490. https://doi.org/10.1007/s10126-007-9007-3
  16. Kardosova A and Machova E. 2006. Antioxidant activity of medicinal plant polysaccharides. Fitoterapia 77, 367-373. https://doi.org/10.1016/j.fitote.2006.05.001
  17. Kikuzaki H and Nakatani N. 1993. Antioxidant effects of some ginger constituents. J Food Sci 58, 1407-1410. https://doi.org/10.1111/j.1365-2621.1993.tb06194.x
  18. Lee SH, Karawita R, Affan A, Lee JB, Lee BJ and Jeon YJ. 2008. Potential antioxidant activites of enzymatic digests from benthic diatoms Achnanthes longipes, Amphora coffeaeformis, and Navicula sp. (Bacillario-phyceae). J Food Sci Nutr 13, 166-175. https://doi.org/10.3746/jfn.2008.13.3.166
  19. Liang S, Liu X, Chen F and Chen Z. 2004. Current microalgal health food R and D activities in China. Hydrobiol 512, 45-48. https://doi.org/10.1023/B:HYDR.0000020366.65760.98
  20. Lindenschmidt RC, Trika AF, Guard ME and Witschi HP. 1986. The effect of dietary butylated hydroxy toluene on liver and colon tumor development in mice. Toxicol 38, 151-160. https://doi.org/10.1016/0300-483X(86)90116-2
  21. Liu F and Ng TB. 2000. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci 66, 725-735. https://doi.org/10.1016/S0024-3205(99)00643-8
  22. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. 1951. Protein measurement with the Folin-Phenol reagents. J Biol Chem 193, 265-275.
  23. Lu F and Foo LY. 1995. Toxicological aspects of food antioxidants. In: Food antioxidants. Madhavi DL, Deshpande SS and Salunkhe DK, eds. Marcel Dekker, New York, U.S.A., 73-146.
  24. Lu Y and Foo LY. 2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem 68, 81-85. https://doi.org/10.1016/S0308-8146(99)00167-3
  25. Marcocci PL, Sckaki A and Albert GM.1994. Antioxidant action of Ginkgo biloba extract EGb 761. Methods Enzymol 234, 462-475. https://doi.org/10.1016/0076-6879(94)34117-6
  26. Moure A, Dominguez, H and Parajo JC. 2006. Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochem 41, 447-456. https://doi.org/10.1016/j.procbio.2005.07.014
  27. Muller HE. 1995. Detection of hydrogen peroxide produced by Microorganism on ABTS-peroxidase medium. Zentralbl Bakteriol Mikrobio Hyg 259, 151-158.
  28. Nagai T, Inoue I, Inoue H and Suzuki N. 2003. Preparation and antioxidant properties of water extract of propolis. Food Chem 80, 29-33. https://doi.org/10.1016/S0308-8146(02)00231-5
  29. Nakamura T, Nagayama K, Uchida K and Tanaka R. 1996. Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicyclis, Fish Sci 62, 923-926. https://doi.org/10.2331/fishsci.62.923
  30. Sherwin ER. 1990. Antioxidants. In: Food additives. Branen R, ed. Marcel Dekker. New York, U.S.A., 139-193.
  31. Siriwardhana N, Jeon YJ, Kim SH, Ha JH, Heo SJ and Lee KW. 2004. Enzymatic hydrolysis for effective extraction of antioxidative compounds from Hizikia fusiformis. Algae 19, 59-68. https://doi.org/10.4490/ALGAE.2004.19.1.059
  32. Spitz TT, Bergman M, Moppes D, Grossman S and Arad MS. 2005. Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J App Phycol 17, 215-222. https://doi.org/10.1007/s10811-005-0679-7
  33. Ukeda H, Shimamura T, Tsubouchi M, Harada Y, Nakai Y and Sawamura M. 2002. Spectrophotometric assay of superoxide anion formed in Maillard reaction based on highly water-soluble tetrazolium salt. Anal Sci 18, 1151-1154. https://doi.org/10.2116/analsci.18.1151
  34. Yang MY, Han YK and Noh BS. 2000. Analysis of lipid oxidation of soybean oil using the portable electronic nose. Korean J Food Sci Technol 9, 146-150.

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