한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제25권1호
  • /
  • Pages.1-5
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  • 2010
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지

한약재 복합추출물과 점토 광물질 혼합제의 Escherichio coli O157:H7에 대한 항균효과

Antimicrobial Activity of Korean Herbal Complex Extract and Clay Mineral Mixture against Escherichia coli O157:H7

  • 이연욱 (주식회사 세농) ;
  • 정원철 (경상대학교 수의과대학 생명과학연구소) ;
  • 차춘남 (경상대학교 산업시스템공학부 공학연구원) ;
  • 김곤섭 (경상대학교 수의과대학 생명과학연구소) ;
  • 이여은 (경상대학교 보건대학원 환경보건학과) ;
  • 김석 (경상대학교 수의과대학 생명과학연구소) ;
  • 이후장 (경상대학교 수의과대학 생명과학연구소)
  • Lee, Yeon-Ok (Senong Co., Ltd.) ;
  • Jung, Won-Chul (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Kim, Gon-Sup (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Yeo-Eun (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2010.01.08
  • 심사 : 2010.01.18
  • 발행 : 2010.03.31
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초록

본 연구는 황련, 금은화, 그리고 백작약 등 한약재 복합 추출물의 E. coli O157:H7에 대한 항균효과와, 한약재 복합추출물과 dioctahedral smectite의 합제의 E. coli O157:H7 감염 마우스에 대한 효과를 평가하기 위해 수행되었다. 한약재 복합추출물, CLP1000을 이용하여 E. coli O157:H7에 대한 항균효과 확인 시험을 수행한 결과, CLP1000 10%와 20%를 첨가한 배지에서 E. coli O157:H7의 성장을 각각 30%와 47%를 억제하는 효과가 관찰되었다. 또한, E. coli O157:H7을 김염시킨 마우스에 CLP1000, dioctahedral smectite (DHS), 그리고 CLP1000와 DHS 합제 (CLPS1000)를 각각 7 일 동안 경시별로 마우스 분변 중의 E. coli O157:H7 균수의 변화를 관찰한 결과, 투여 3일째에, 10% CLPS1000을 투여한 군에서 대조군과 비교하여 E. coli O157:H7의 균수가 유의성 있게 감소하였으며(p < 0.05), 투여 7일째에는 10% CLP1000 (p < 0.05)과 10% CLPS1000을 투여한 군에서 대조군과 비교하여 E. coli O157:H7의 균수가 유의성 있게 감소하는 결과를 나타내었다. 따라서 본 연구의 결과로부터, CLPS1000을 E. coli O157:H7에 감염된 마우스에 투여할 경우 감염증상을 완화 시킬 수 있을 것으로 기대된다.

The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma, Lonicerae Flos, and Paeonia japonica (1:1:1) extracts (CLP1000). Also, the effectiveness of CLP1000, dioctahedral smectite (DHS), and the combination of CLP1000 and DHS (CLPS1000) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 10% and 20% CLP1000 were inhibited the growth of E. coli O157:H7 by 30% and 47%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were orally administered with saline, 10% CLP1000, 10% DHS, and 10% CLPS1000, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CLP1000 (p < 0.05), DHS (p < 0.05) and CLPS1000 (p < 0.001). On the 7th day, CLP1000 (p < 0.05) and CLPS1000 p < 0.001) administration significantly decreased the number of E. coli O157:H7. According to the results of the present study, administration of CLPS1000 to mice can reduce the severity of E. coli O157:H7 infection. Also, it is suggested that CLPS100 represents a good candidate for the treatment of enteric infections in domestic animals.

키워드

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