Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Co-treatment with Demecolcine and BMI-1026, a Potent cdk1 Inhibitor, Induces the Enucleation of Murine Oocytes

  • Choi, Tae-Saeng (Department of Microbiology, College of Medicine, Dankook University)
  • Received : 2010.03.02
  • Accepted : 2010.03.23
  • Published : 2010.03.31
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Abstract

Oocyte enucleation is essential for somatic cell nuclear transfer (SCNT) in the production of cloned animals or embryonic stem cells from adult somatic cells. Most studies of oocyte enucleation have been performed using micromanipulator-based techniques, which are technically demanding, time-consuming, and expensive. Several recent studies have used chemical-induced oocyte enucleation; however, each has been plagued by low efficiency and toxicity. In this study, I found that the co-treatment of murine oocytes with demecolcine and BMI-1026, a potent cdk1 inhibitor, resulted in a high enucleation rate (97%). This method is entirely independent of a micromanipulator and is suitable for the large-scale production of enucleated oocytes. This new method of enucleation will be useful in SCNT and in the development of handmade cloning techniques.

Keywords

Acknowledgement

Supported by : Dankook University

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