Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Effect of Ultraviolet (UV-B) on Antioxidants and Antioxidative Enzymes in Garden Balsam(Impatiens balsamina L.)

자외선(UV-B)이 봉선화(Impatiens balsamina L.)의 항산화제 및 항산화 효소에 미치는 영향

  • Kim, Hak-Yoon (Department of Global Environment, Keimyung University)
  • Received : 2010.06.04
  • Accepted : 2010.06.21
  • Published : 2010.06.30
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Abstract

To investigate the effects of ultraviolet(UV-B) on growth and biochemical defense responses of plant, garden balsam (Impatiens balsamina L.) was subjected to enhanced UV-B irradiation [daily dose: 0.02 (No UV-B) and 11.34 (enhanced UV-B) kJ $m^{-2}$ ; $UV-B_{BE}$] for 3 weeks. Enhanced UV-B drastically inhibited leaf area as well as dry weight of garden balsam. The content of malondialdehyde was significantly increased by about 50% after 3 weeks of UV-B irradiation. The ratios of dehydroascorbate/ascorbate and oxidized glutathione/reduced glutathione were also considerably increased by UV-B irradiation. Three major polyamines of garden balsam leaves: putrescine, spermidine and spermine were observed. All polyamine contents were increased with UV-B irradiation. The enzyme (superoxide dismutase, ascorbate peroxidase etc.) activities of garden balsam were increased by the UV-B enhancement. Based on the results, enhanced UV-B caused oxidative stress in garden balsam and biochemical protection responses might be activated to prevent from damaging effects of oxidative stress generated by UV-B irradiation.

UV-B 처리가 봉선화 식물에 미치는 영향과 식물의 생화학적 방어반응을 조사하고자 3주간 UV-B (11.34 kJ $m^{-2}$) 조사 실험을 수행하였다. UV-B 처리에 의해 봉선화의 엽면적 및 건물중이 약 40% 정도 감소하였으며, MDA 함량은 50% 정도 증가한 것으로 나타났다. Glutathione 및 ascorbate acid 함량은 UV-B에 의해 산화형이 증가하고 환원형이 감소하였다. 봉선화 잎에는 주로 3종류의 polyamine이 존재하였으며, 3종류 모두 UV-B에 의해 증가하는 것으로 나타났다. 항산화효소인 SOD, AP, GR 및 GP의 활성이 UV-B 처리에 의해 크게 증가하였다. 이상의 결과로 볼 때 UV-B 증가는 식물체내 산화스트레스를 일으키며, 이에 대해 식물의 생화학적 방어반응이 작용하는 것으로 사료된다.

Keywords

References

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