KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Stabilization of Astaxanthin Using Nanoliposome

나노리포좀을 이용한 astaxanthin의 안정화

  • Yoo, Ji-Min (R&D Center, Cotde Co., Ltd.) ;
  • Kim, So-Young (Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Ah (Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Hye (Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Choi, Sun-Ju (Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University) ;
  • Jeong, Yoon-Joo (R&D Center, Cotde Co., Ltd.) ;
  • Ha, Byung-Jhip (R&D Center, Cotde Co., Ltd.) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology, Center for Food Function and Safety, Hoseo University)
  • 유지민 ((주)콧데 기업부설연구소) ;
  • 김소영 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 조은아 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 조은혜 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 최선주 (호서대학교 식품생물공학과, 식품기능안전연구센터) ;
  • 정윤주 ((주)콧데 기업부설연구소) ;
  • 하병집 ((주)콧데 기업부설연구소) ;
  • 채희정 (호서대학교 식품생물공학과, 식품기능안전연구센터)
  • Received : 2010.03.05
  • Accepted : 2010.04.15
  • Published : 2010.04.28
Download PDF
⟨ Previous Next ⟩

Abstract

Astaxanthin is an unsaturated compound with a double bond. So it is easily decayed by heat and oxidation (light) during its storage and processing of it. Nanoliposome formulation technology was utilized to improve the stability of astaxanthin. Nanoliposome preparation conditions were established and the stability of astaxanthin encapsulated nanoliposome and free astaxanthin was investigated. Thermal stability and UV-stability of astaxanthin encapsulated nanoliposome increased up to two times and tree times, respectively. Astaxanthin encapsulated nanoliposome could be used as a stable functional material for industrial purposes.

본 연구에서는 이중결합을 가진 불포화화합물로 제조나 저장 시 열과 산화 (빛)에 의해 쉽게 파괴되어 활성이 감소하여 응용범위에 한계가 있는 아스타잔틴의 안정성 향상을 위하여 나노리포좀 제형기술을 이용하였다. 제형안정성 평가를 통하여 리포좀 제조조건 및 조성비를 확립하였고, 포접나노리포좀을 제조하여 포접전의 아스타잔틴과 안정성을 비교 검토하였다. 아스타잔틴을 포접하여 포접나노리포좀 제조 시포접전의 아스타잔틴에 비해 열에 대한 안정성이 2배 정도 향상되고, UV 안정성 또한 3배 향상된 것을 확인하였다. 이상의 결과로부터 아스타잔틴을 포접한 포접나노리포좀은 안정성이 우수한 기능성 원료로서 산업적으로 용도를 넓히고 부가가치를 더욱 향상시킬 수 있을 것으로 기대된다.

Keywords

References

  1. Shimidzu, N., M. Goto, and W. Miki (1996) Carotenoids as singlet oxygen quenchers in marine organisms. Fish. Sci. 62: 134-137. https://doi.org/10.2331/suisan.62.134
  2. Hong, S. P., M. H. Kim, and J. K. Hwang (1998) Biological functions and production technology of carotenoids. J. Kor. Soc. Food Sci. Nutr. 27: 1279-1306.
  3. During, A., A. Nagao, and J. Terao (1998) $\beta$-carotene 15, 15-deoxygenase activity and cellular retinal-binding protein type II level are enhanced by dietary unsaturated triacylglycerols in rat intestines. J. Nutr. 128: 1614-1619. https://doi.org/10.1093/jn/128.10.1614
  4. Jorgensen, K. and L. Skibsted (1993) Carotenoid scavenging radicals. Effect of carotenoid structure and oxygen partial pressure on antioxidative activity. Z. Lebensm. Unters Forsch. 196: 423-429. https://doi.org/10.1007/BF01190806
  5. Naguib, Y. (2000) Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Chem. 48: 1150-1154. https://doi.org/10.1021/jf991106k
  6. Lim, B. P., A. Nagao, J. Terao, K. Tanaka, T. Suzuki, and K. Takama (1992) Antioxidant activity of xanthophylls on peroxyl radical-mediated phospholipidperoxidation. Biochim. Biophys. Acta 1126: 178-184. https://doi.org/10.1016/0005-2760(92)90288-7
  7. Nakagawa, K., S. Kang, D. Park, G. Handelman, and T. Miyazawa (1997) Inhibition by beta-carotene and astaxanthin of NADPH-dependent microsomal phospholipid peroxidation. J. Nutr. Sci. Vitamin 43: 345-355. https://doi.org/10.3177/jnsv.43.345
  8. Chew, B. P., J. S. Park, M. W. Wong, and T. S. Wong (1999) A comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res. 19: 1849-1854.
  9. Jyonouchi, H., S. Sun, K. Iijima, and M. Gross (2000) Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer. 36: 59-65. https://doi.org/10.1207/S15327914NC3601_9
  10. Marnett, L. J. (1987) Peroxyl free astaxanthin radicals: potential mediators of tumor initiation and promotion. Carcinogenesis 8: 1365-1373. https://doi.org/10.1093/carcin/8.10.1365
  11. Batieha, A. (1993) Serum micronutrients and the subsequent risk of cervical cancer in a population-based nested case-control cancer epidemiology. Biomarkers Prev. 2: 335-339.
  12. Bertram, J. S. (1991) Cancer prevention by retinoids and carotenoids: proposed role of GAP junctional communication. PP. 31-50. In: Vitamins and Minerals in the Prevention and Treatment of Cancer. M. M.Jacobs (ed.), CRC Press, Boca Raton, USA.
  13. Tomita, Y., K. Himeno, K. Nomoto, and H. Endo (1987) Augmentation of tumor immunity against synergistic tumors in mice by beta-carotene. J. Natl. Cancer Inst. 78: 679-681.
  14. Kistler, A. (2002) Metabolism and CYP-inducer properties of astaxanthin in man and primary human hepatocytes. Arch. Toxicol. 75: 665-675. https://doi.org/10.1007/s00204-001-0287-5
  15. Kurihara, H. (2002) Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancermetastasis in mice treated with restraint stress. Life Sci. 70: 2509-2520. https://doi.org/10.1016/S0024-3205(02)01522-9
  16. Bendich, A. (1991) Non vitamin a activity of carotenoids: immuno enhancement. Food Sci. Technol. Res. 2: 127-130. https://doi.org/10.1016/0924-2244(91)90648-3
  17. Bendich, A. (1989) Carotenoids and the immune response. J. Nutr. 119: 112-115. https://doi.org/10.1093/jn/119.1.112
  18. Bendich, A. (1990) Carotenoids and the immune system. pp. 323-335. In: N. I. Krinsky (ed.). Carotenoids Chemistry and Biology. Plenum Press, New York, USA.
  19. Tomita, Y., H. Jyonouchi, R. W. Engelman, N. K. Day, and R. A. Good (1993) Preventive action of carotenoids on the development of lymphadenopathy and proteinuria in MRL-lpr/lpr mice. Autoimmunity 16: 95-102. https://doi.org/10.3109/08916939308993316
  20. Breimer, L. H. (1990) Molecular mechanisms of oxygen radical carcinogenesis and mutagenesis: the role of DNA base damage. Mol. Carcinog. 3: 188-197.
  21. Yonouchi, J. (1993) Astaxanthin enhances in vitro antibody production to T-dependent antigens without facilitating polyclonal B-cell activation. Nutr. Cancer 19: 269-280. https://doi.org/10.1080/01635589309514258
  22. Kritchevsky, S. B. (1999) $\beta$-Carotene, carotenoids and the prevention of coronary heart disease. J. Nutr. 129: 5-8.
  23. Frei, B. (1995) Cardiovascular disease and nutrient antioxidants: role of low-density lipoprotein oxidation Crit. Rev. Food Sci. Nutr. 35: 83-98. https://doi.org/10.1080/10408399509527689
  24. Aghdassi, E. and J. P. Allard (2000) Breath alkanes as a marker of oxidative stress in different clinical conditions. Free Radic. Biol. Med. 28: 880-886. https://doi.org/10.1016/S0891-5849(00)00189-1
  25. O'Connor, I. and N. O'Brien (1998) Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in cultured fibroblasts. J. Dermatol. Sci. 16: 226-230. https://doi.org/10.1016/S0923-1811(97)00058-3
  26. Jacques, P. (1999) The potential preventive effects of vitamins for cataract and age-related macular degeneration. Int. J. Vitam. Nutr. Res. 69: 198-205. https://doi.org/10.1024/0300-9831.69.3.198
  27. Lyle, B. J. (1999) Antioxidant intake and risk of incident age-related nuclear cataracts in the beaver dam eye study. Am. J. Epidemiol. 149: 801-809. https://doi.org/10.1093/oxfordjournals.aje.a009895
  28. Seddon, J. M. (1994) Dietary carotenoids, vitamins A, C, and E and advanced agerelated macular degeneration. J. Am. Med. Assoc. 272: 1413-1420. https://doi.org/10.1001/jama.272.18.1413
  29. Landrum, J. T. (1999) Analysis of zeaxanthin distribution within individual human retinas. Meth. Enzymol. 299: 457-467. https://doi.org/10.1016/S0076-6879(99)99043-2
  30. Gradelet, S. (1998) Dietary carotenoids inhibit aflatoxin B1-induced liver preneoplastic foci and DNA damage in the rat: role of the modulation of aflatoxin B1 metabolism. Carcinogenesis 19: 403-411. https://doi.org/10.1093/carcin/19.3.403
  31. Jung, S. Y. (2005) Skin Anti-wrinkle ingredients containing astaxanthin. Korea Patent 10, 2005, 0096556.
  32. Chen, X. R., Z. Chen, C. Guo, and P. Li (2007) The preparation and stability of the inclusion complex of astaxanthin with $\beta$-cyclodextrin. Food Chem. 101: 1-5. https://doi.org/10.1016/j.foodchem.2005.12.053
  33. Yun, M. J., J. H. Kim, H. Y. Choi, S. K. Kim, and Y. S. Gu (2003) The method for making astaxanthin water-soluble. Korea Patent 10, 2004, 0098887.
  34. Lee, C. K. (2003) Process development for astaxanthin production by microalgae. Research Report, Ministry of Maritime Affairs & Ficheries, Korea.
  35. Higuera-Ciapara, I., L. Felix-Valenzuela, F. M. Goycoolea, and W. Arguelles-Monal (2004) Microencapsulation of astaxanthin in a chitosan matrix. Carbohydr. Polymers 56: 41-45. https://doi.org/10.1016/j.carbpol.2003.11.012
  36. Kittikaiwan, P., S. Powthongsook, P. Pavasant, and A. Shotipruk (2007) Encapsulation of Haematococcus pluvialis using chitosan for astaxanthin stability enhancement. Carbohydr. Polymers 13: 1-8.
  37. Chansiri, G., R. T. Lyons, M. V. Patel, and S. L. Hem (1999) The reversibility of the vitamin c redox system: electrochemical reason and biological aspects. J. Pharm.Sci. 88: 454-458. https://doi.org/10.1021/js980293i
  38. Meyer, R. (2005) Delivery System Handbook for Personal and Cosmetic Products. William Andrew Publishing, NY, USA.
  39. Yeom, E. J. (2003) Studies on the Using Liposome Cosmetics Formulation Technology and Its Effect on the Transdermal Permeation. M.S. Thesis. Konkuk University, Seoul, Korea.
  40. Lee, K. Y. (1999) The Stabilization of Ascorbic Acid Using Liposome. Soongsil University, Seoul, Korea.
  41. Kim, S. Y., E. A. Cho, J. M. Yoo, M. J. In, and H. J. Chae (2008) Extraction and analysis of astaxanthin from Haematococcus pluvialis using sonication. J. Korea Soc. Food Sci. Nutr. 37: 1363-1368. https://doi.org/10.3746/jkfn.2008.37.10.1363
  42. Chansiri, G., R. T. Lyons, M. V. Patel, and S. L. Hem (1999) Effect of surface change on the stability of oil/ water emulsions during steam sterilization. J. Pharm. Sci. 88: 454-458. https://doi.org/10.1021/js980293i
  43. Kim, J. D. (2005) A Study of Nanoceramide and It's Effects. M.S. Thesis. Sookmyung Women's University, Seoul, Korea.
  44. Snodderly, D. M. (1995) Evidence for protection against age-related macular degeneration by carotenoids andantioxidant vitamins. Am. J. Clin. Nutr. 62: 1448-1461. https://doi.org/10.1093/ajcn/62.6.1448S
  45. Gregoriadis, G. (1988) Liposome as Drug Carriers, Recent Trends and Progress. John Wiley and Son, NY, USA.
  46. Gregor, C. (2004) Lipoid vesicles and other colloids as drug carriers on the skin. Adv. Drug Delivery Rev. 56: 675-711. https://doi.org/10.1016/j.addr.2003.10.028