Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Mechanism of Daidzein on Helicobacter pylori Growth

Helicobacter pylori 의 생육에 대한 Daidzein의 저해 특성

  • Bae, Kyung-Mi (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Lee, Ju-Youn (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Lee, Hee-Seob (Dept. of Food Science and Nutrition, Pusan National University)
  • 배경미 (부산대학교 식품영양학과) ;
  • 이주연 (부산대학교 식품영양학과) ;
  • 이희섭 (부산대학교 식품영양학과)
  • Received : 2010.07.05
  • Accepted : 2010.07.09
  • Published : 2010.07.31
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Abstract

This study was conducted to investigate the inhibitory effects of daidzein against H. pylori and its cholesterol $\alpha$-glucosyltransferase ($CHL{\alpha}GcT$). $CHL{\alpha}GcT$ is responsible for the production of $\alpha$-glucosyl cholesterol which constitutes more than 25% of cell wall lipids in H. pylori, and it has been suggested that it is essential for H. pylori viability. $CHL{\alpha}GcT$ was inhibited by daidzein, in a dose-dependant manner, of which $IC_{50}$ value was $128.5\;{\mu}M$. Daidzein also showed the inhibitory effect toward H. pylori growth by paper disc diffusion assay. Therefore, it is thought that the inhibition of daidzein on $CHL{\alpha}GcT$ was related to its anti-Helicobacter activity.

본 연구에서는 대두 isoflavone의 하나인 daidzein의 콜레스테롤 당전이 효소에 대한 저해효과와 헬리코박터에 대한 항균력을 측정하였다. 콜레스테롤 당전이 효소는 헬리코박터 파이로리의 세포벽 지질성분을 구성하는 $\alpha$-glucosyl cholesterol을 생성하는 데 관여하는 효소로 헬리코박터의 생육에 있어서 중요한 역할을 하고 있다. Daidzein을 농도별로 처리하였을 경우, 콜레스테롤 당전이 효소의 활성은 daidzein의 농도에 의존적으로 저해를 나타내었으며, daidzein의 $IC_{50}$값은 $128.5\;{\mu}M$이었다. 또한 disc diffusion 방법을 이용하여 항균실험에 있어서도 daidzein은 헬리코박터의 생육을 억제하였다. 이상의 결과를 통하여 daidzein의 효소저해 활성은 헬리코박터의 생육 저해와 밀접한 연관이 있는 것으로 판단된다.

Keywords

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