Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus

노루궁뎅이(Hericium erinaceus) 자실체 추출 조다당류의 생쥐 Sarcoma 180에 대한 면역증강 및 항암 효과

  • Received : 2010.02.26
  • Accepted : 2010.04.12
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of $50\;{\mu}g/ml$ compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of $200\;{\mu}g/ml$. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.

노루궁뎅이는 담자균문, 민주름버섯목, 산호침버섯과에 속하는 식의약용버섯으로 예로부터 위궤양이나 위암의 치료에 효과가 있으며 생쥐의 Sarcoma 180과 Ehrlich Sarcoma에도 효과가 있다고 알려져 왔다. 노루궁뎅이의 자실체로부터 중성염용액, 열수 및 메탄올 등을 이용하여 조다당류를 추출하고 ICR mice에 주사하여 항암 및 면역증강 효과를 조사하였다. Sarcoma 180, HepG2, HT-29, NIH3T3 등의 세포에 대한 세포독성을 조사한 결과 $10{\sim}2,000\;{\mu}g/ml$의 조다당류 농도에서 각각의 암세포는 세포독성을 거의 나타내지 않았다. Sarcoma 180으로 접종된 ICR mouse에 자실체에서 추출한 각각의 조다당류를 투여한 실험군은 대조군에 비해 수명이 각각 29.1~54.1% 연장되었다. 노루궁뎅이 자실체를 중성염 용액으로 추출한 조다당류는 $50\;{\mu}g/ml$의 농도에서 비장세포의 수를 2.9배 증가시켰으며 또한 중성염 추출 조다당류를 $200\;{\mu}g/ml$의 농도로 투여한 실험군 생쥐의 B 임파구 alkaline phosphatase의 활성은 대조군에 비해 5.5배의 증가하였다. 중성염 용액 추출 조다당류를 50 mg/kg body weight의 농도로 투여한 실험군 생쥐의 총 복강 세포 수와 백혈구의 수는 대조군에 비하여 각각 4배와 2.3배로 증가하였다. 또한 면역 관련 장기인 간, 비장 및 흉선의 체중에 대한 무게 비율은 대조군에 비하여 유의성을 보이지 않았다. 따라서 노루궁뎅이의 자실체에서 추출한 조다당류는 생쥐의 면역을 증강시키고 생쥐의 Sarcoma 180에 대해 항암효과를 나타내었다.

Keywords

References

  1. Allison, D. C. and P. Ridolpho. 1980. Use of a trypan blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable cells. J. Histochem. Cytochem. 28, 700-703. https://doi.org/10.1177/28.7.6156203
  2. Arthur, C. and M. D. Guyton. 1986. Textbook of medical physiology. 7th eds. W. B. Saunder Company. pp. 51-59.
  3. Chihara, G., Y. Maeda, G. Hamuro, Y. Arai, and F. Fukuoka. 1969. Inhibition of mouse Sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing. Nature 222, 687-688. https://doi.org/10.1038/222687a0
  4. Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo, and I. D. Yoo. 1995. Immuno-stimulating polysaccharides from the fruiting bodies of Fomitella fraxinea (1)-Characterization of polysaccharides extracted with neutral sodium chloride solution. Kor. J. Mycol. 23, 332-339.
  5. Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo, and I. D. Yoo. 1995. Immuno-stimulating polysaccharides from the fruiting bodies of Fomitella fraxinea (2)-Isolation and characterization of hot-water extracted with polysaccharides. Kor. J. Mycol. 23, 340-347.
  6. Choi, Y. I., K. W. Lee, H. Hur, W. Y. Lee, and T. S. Lee. 2008. Immuno-potentiating and antitumor effects against mouse Sarcoma 180 by crude polysaccharides extacted from fruiting body of Russula rosacea. Kor. J. Mycol. 36, 84-92. https://doi.org/10.4489/KJM.2008.36.1.084
  7. Deonizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  8. Fukuda, K., T. Uematsu, A. Hamada, and S. Akiya. 1975. The polysaccharide from Lampteromyces Japonicus. Chem. Pharm. Bull. 23, 1955-1961. https://doi.org/10.1248/cpb.23.1955
  9. Ji, I. H. and M. N. Kim. 2000. Antimutagenic and cytotxicity effects of Phellinus linteus extracts. J. Kor. Soc. Food Sci. Nutr. 29, 322-330.
  10. Hur, H., Y. I., Choi, and T. S. Lee. 2008. Antitumor and immuno-potentiating activity against mouse Sarcoma 180 by crude polysaccharides extracted from fruiting body of Tricholoma matsutake. J. Life Sci. 18, 1290-1298. https://doi.org/10.5352/JLS.2008.18.9.1290
  11. Jin, M. L. 1996. A study on the activation of immune cell and transcription factor of pine nut mushroom. Ph. D thesis of Seoul National University Graduate School. pp. 1-121.
  12. Kawakishi, H., A. Shimada, S. Hosokawa, and H. Mori. 1996. Erinacenes, E, F, G, stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceus. Tetrahedron Lett. 37, 7399-7402. https://doi.org/10.1016/0040-4039(96)01687-5
  13. Kim. B. K., E. K. Park, and M. J. Shim. 1979. Studies on constituents of higher fungi of Korea (XXIV), antineoplastic activities of Coriolus versicolor (Fr.). Quel, Pleurotus ostreatus (Fr.) Kummer and Lentnus edodes (Berk.) Sing. Arch. Pharm. Res. 2, 145-149. https://doi.org/10.1007/BF02959042
  14. Kim, S. B., K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee, and T. S. Lee. 2006. Inhibitive effect of mouse Sarcoma 180 by crude polysaccharides extracted from fruiting Body of Armillaria mellea. Kor. J. Mycol. 34, 98-104. https://doi.org/10.4489/KJM.2006.34.2.098
  15. Kim, S. B., K. W. Lee, W. Y. Lee, and T. S. Lee. 2007. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from fruiting Body of Oudemansiella radicata. Kor. J. Mycol. 35, 109-114. https://doi.org/10.4489/KJM.2007.35.2.109
  16. Lee, G. W., H. Y. Kim, W. H. Lee, and T. S. Lee. 2007. Antitumor and immuno-modulatory effect against mouse sarcoma 180 of crude polysaccharides extracted from fruiting Body of Armillaria tabescens. Kor. J. Myco. 35, 101-108. https://doi.org/10.4489/KJM.2007.35.2.101
  17. Lee, G. W., H. Y. Kim, H. Hur, M. W. Lee, M. J. Shim, M. Y. Lee, and T. S. Lee. 2008. Antitumor and immuno-modulatory effect of crude polysaccharides from fruiting body of Tremella aurantialba against mouse Sarcoma 180. Kor. J. Mycol. 36, 66-74. https://doi.org/10.4489/KJM.2008.36.1.066
  18. Lee, K. Y., M. J. Han, S. Y. Park, and D. H. Kim. 2000. In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. Kor. J. Food Sci. Technol. 32, 477-480.
  19. Mizno, T. 1992. Antitumor active polysaccharides isolated from the fruiting body of Hericium erinaceus, an edible and medicinal mushroom called yamabushitake or houtou. Biosci. Biochem. 56, 347-348 https://doi.org/10.1271/bbb.56.347
  20. Mossman, B. T. 1983. In vitro approaches for determining mechanism of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect. 53, 155-161. https://doi.org/10.2307/3429628
  21. Oh, Y. H., W. Y. Lee, M. W. Lee, M. J. Shim, and T. S. Lee. 2004. Immuno-modulatory and antitumor effect of crude polysaccharides extracted from sclerotium of Grifola umbellata. Kor. J. Mycol. 32, 23-30. https://doi.org/10.4489/KJM.2004.32.1.023
  22. Oh, Y. H., S. B. Kim, K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee, and T. S. Lee. 2006. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Tremella fuciformis. Kor. J. Mycol. 34, 105-111. https://doi.org/10.4489/KJM.2006.34.2.105
  23. Ohno, N., Y. Arai, I. Suzuki, and T. Yadomae. 1986. Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens. J. Pharmacobio-Dyn. 9. 593-599. https://doi.org/10.1248/bpb1978.9.593
  24. Park, W. H. and H. D. Lee. 2002. Mushrooms in Korea. Kyohak publishing Co., Ltd., Seoul.
  25. Shim, M. J. 1980. Stimulating effects of Coriolus versicolor constituents on immune response. Kor. J. Mycol. 8, 115-116
  26. Shim, S. M., K. H. Im, W. Y. Lee, J. W. Kim, M. J. Shim, M. W. Lee, and T. S. Lee. 2003. Studies on immuno-modulatory and antitmor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31, 155-160. https://doi.org/10.4489/KJM.2003.31.3.155
  27. Shim, S. M., K. W. Im, J. W. Kim, W. Y. Lee, H. W. Kim, M. W. Lee, and T. S. Lee. 2003. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Daedaleopsis tricolor. Kor. J. Mycol. 31, 161-167. https://doi.org/10.4489/KJM.2003.31.3.161
  28. Ying, J. Z., X. L. Mao, Q. M. Ma, Y. C. Zong, and H. A. Wen. 1987. Icons of medical fungi from China. Science Press, Beijing, China.

Cited by

  1. The difference of occurring pattern of Hericium erinaceus by pinheading induction methods vol.13, pp.1, 2015, https://doi.org/10.14480/JM.2015.13.1.11
  2. Characteristics of Hericium erinaceus fruit body genesis in winter vol.14, pp.2, 2016, https://doi.org/10.14480/JM.2016.14.2.39
  3. Physiological activities of Lespedeza cuneata extracts vol.21, pp.6, 2014, https://doi.org/10.11002/kjfp.2014.21.6.844
  4. Anticancer potential of Hericium erinaceus extracts against human gastrointestinal cancers vol.153, pp.2, 2014, https://doi.org/10.1016/j.jep.2014.03.003
  5. Immuno-stimulating Activities of Skipjack Tuna Katsuwonus pelamis Cooking Juice Concentrates on Mouse Macrophages and Spleen Cells vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0776
  6. Using automated container type culture system Hericium erinaceum mushroom production technology vol.14, pp.4, 2016, https://doi.org/10.14480/JM.2016.14.4.249
  7. Phylegenetic analysis of Hericium species based on ITS rDNA sequences vol.12, pp.4, 2014, https://doi.org/10.14480/JM.2014.12.4.251