The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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A Chromosome Aberration Test of HMC05 on Cultured Chinese Hamster Lung Cells

HMC05의 배양 Chinese Hamster Lung 세포를 이용한 염색체이상 시험

  • Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Received : 2010.01.19
  • Accepted : 2010.03.22
  • Published : 2010.03.30
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Abstract

Objectives : We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods : Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500, $5,000\;{\mu}g/ml$), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results : HMC05 did not show any aberrant metaphase. However, there were significant (p < 0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions : These results indicate that HMC05 formula does not show any toxicity in chromosome aberration test.

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References

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