The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Inhibitory Effects of Herb and Seeds of Oenothera odorata on the Melanogenesis

월견초 및 월견자의 멜라닌 생성 억제효과

  • Choi, Mi-Eun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University)
  • Received : 2010.03.07
  • Accepted : 2010.04.07
  • Published : 2010.04.25
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Abstract

목적 : 월견초는 다량의 불포화 지방산인 리놀렌산과 감마 리놀렌산을 함유하고 있으며, 천식성 기침이나 아토피피부염에 효능이 있는 것으로 알려져 있다. 본 연구는 월견초의 전초와 종자 추출물의 피부 멜라닌 합성에 대한 억제 효과를 조사하였다. 방법 : B16F10 멜라닌세포주를 이용하여 멜라닌, tyrosinase 활성 및 세포생존율을 측정하였다. 또한 멜라닌 합성- 관련효소인 tyrosinase, TRP-1, TRP-2의 단백질발현과 $\alpha$-MSH를 처리하여 색소침착을 유도 한 뒤 단백질 발현을 조사하였다. 결과 : 월견자는 B16F10 세포의 멜라닌 합성을 $5\;{\mu}g/ml$와 $10\;{\mu}g/ml$ 농도에서 각각 대조군의 81.3%, 68.3%로 억제하였고 tyrosinase의 활성도 이와 유사하게 억제하였다. 멜라닌 합성-관련효소들의 단백질발현을 관찰한 결과 월견초와 월견자는 tyrosinase 발현을 억제하였으며 TRP-1과 TRP-2의 발현에는 영향을 주지 않았다. 특히 $\alpha$-MSH에 의한 과색소 유도 시 tyrosinase 발현이 현저하게 감소되었으며, 월견자의 멜라닌 합성 억제 효과가 월견초 보다 높게 나타났다. 결론 : 이상의 연구 결과 월견자는 멜라닌세포의 tyrosinase 단백질 발현과 tyrosinase 활성을 억제하여 멜라닌 생성을 감소시키는 것으로 사료된다.

Keywords

References

  1. Masago Ishikawa, Ichiro Kawase, Fumio Ishii. Glycine Inhibits Melanogenesis in vitro and Causes Hypopigmentation in vivo. Biol Pharma Bull. 2007;30:2031-6. https://doi.org/10.1248/bpb.30.2031
  2. Sato K, Takahashi H, Iraha R, Toriyama M. Down-Regulation of Tyrosinase Expression by Acetyksalicylic Acid in Murine B16 Melanoma. Biol & Pharma Bull. 2008;31:33-7. https://doi.org/10.1248/bpb.31.33
  3. Choi YJ, kim JD. A study on the Propensity to Consume Oriental Herbal Cosmetics. J Soc Cosmet Sci. 2006;32:283-90.
  4. Wang KH, Lin RD, Hsu FL, Huange YH, Changf HC, Huangd CY, Lee MH. Cosmetic applications of selected traditional Chinese herbal medicines. J Ethnopharmacol. 2006;106:353-9. https://doi.org/10.1016/j.jep.2006.01.010
  5. Gunstone FD. Camma-Linolenic Acid-Occurrence and Physical and Chemical Properties. Prog Lipid Res. 1992;31:145-61. https://doi.org/10.1016/0163-7827(92)90007-6
  6. Horrobin DF. Nutritional and Medical Importance of Gamma-Linolenic Acid. Prog Lipid Res. 1992;31(2):163-94. https://doi.org/10.1016/0163-7827(92)90008-7
  7. Barcelli UO, Weiss M, Beach D, Motz A, Thompson B. High Linoleic Acid Diets Ameliorate Diabetic Nephropathy in Rats. Am J Kidney Dis. 1990;16:244-51. https://doi.org/10.1016/S0272-6386(12)81024-9
  8. Deferne JL, Leeds AR. The Antihypertensive Effects of Dietary Supplementation with a 6-Desaturated Essential Fatty Acid Concentrate as Compared with Sunflower Seed Oil. J Human Hypertens. 1992;6:113-9.
  9. Kernoff PBA, Willis AL, Stone KJ, Davies JA, Nichols GP. Antithrombotic Potential of Dihomo-Gamma-Linolenic Acid in Man. Br Med J. 1977;2:1441-4. https://doi.org/10.1136/bmj.2.6100.1441
  10. Wright S, Burton JL. Oral Evening Primrose Seed Oil Improves Atopic Eczema. Lancet. 1982;57:1120-2.
  11. Kunkel SL, Ogawa H, Ward PA, Zurier RB. Suppression of Chronic Inflammation by Evening Primrose Oil. Prog Lipid Res. 1981;20:885-8. https://doi.org/10.1016/0163-7827(81)90165-X
  12. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 1985;45(4):14748.
  13. Martinez-Esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC. Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. Eur J Biochem. 1998;255(1):139-46. https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  14. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  15. Hunt G, donatien PD, Lunec J. Cultured human melanocytes respond to MSH peptides and ACTH. Pigment Cell Res. 1994;7:217-21. https://doi.org/10.1111/j.1600-0749.1994.tb00052.x
  16. Kim YJ, No JK, Lee JH, Chung HY. 4,4'-Dihydroxybiphenyl as a new potent tyrosinase inhibitor. Biol Pharm Bull. 2005;28:323-7. https://doi.org/10.1248/bpb.28.323
  17. Aoki Y, Tanigawa T, Abe H, Fujiwara Y. Melanogenesis inhibition by an oolong tea extract in B16 mouse melanoma cells and UV-induced skin pigmentation in brownish guinea pigs. Biosc Biotechnol Biochem. 2007;71:1879-85. https://doi.org/10.1271/bbb.70099
  18. Wang N, Hebert DN, Tyrosinase maturation through the mammalian secretory pathway: bring color to life. Pigment Cell Res. 2006;19:3-18. https://doi.org/10.1111/j.1600-0749.2005.00288.x
  19. Maeda K, Fukuda M. In vivo effectiveness of several whitening cosmetic components in human melanocytes. J Soc Cosmet Chem. 1991;42:361-8.
  20. Abdel-Malek Z, Swope VB, Suzuki I, Akcali C, Harriger MD, Boyce ST, Urabe K, Hearing VJ. Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc Natl Acad Sci U.S.A. 1995;92(5):1789-93. https://doi.org/10.1073/pnas.92.5.1789
  21. Galibert MD, Carreira S, Goding CR. The Usf-1 transcription factor is a novel target for the stress-responsive p38 kinase and mediates UV-induced Tyrosinase expression. EMBO J. 2001;20(17):5022-31. https://doi.org/10.1093/emboj/20.17.5022
  22. Bellei B, Maresca V, Flori E, Pitisci A, Larue L, Picardo M. p38 Regulates Pigmentation via Proteasomal Degradation of Tyrosinase. J Biol Chem. 2010;285(10):7288-99. https://doi.org/10.1074/jbc.M109.070573
  23. Virador VM, Muller J, Wu X, Abdel-Malek ZA, Yu ZX, Ferrans VJ, Kobayashi N, Wakamatsu K, Ito S, Hammer JA, Hearing VJ. Influence of alpha-melanocyte-stimulating hormone and ultraviolet radiation on the transfer of melanosomes to keratinocytes. FASEB J. 2002;16:105-7.
  24. Kim KS, Kim JA, Eom SY, Lee SH, Min KR, Kim YS. Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression. Pigment Cell Res. 2006;19:90-8. https://doi.org/10.1111/j.1600-0749.2005.00281.x