DOI QR코드

DOI QR Code

백강균의 분생자병속 형성 조건

Conditions for Formation of Synnemata from Beauveria bassiana

  • 이기만 ((주)마이코플러스) ;
  • 남성희 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤철식 ((주)마이코플러스) ;
  • 전지영 ((주)마이코플러스) ;
  • 여주홍 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이광길 (농촌진흥청 국립농업과학원 농업생물부)
  • 투고 : 2010.02.22
  • 심사 : 2010.03.15
  • 발행 : 2010.03.30

초록

백강균(Beauveria bassiana)의 분생자병속 형성 조건 구명을 위해 본 실험을 수행하였다. 균은 제주도 설악산 일대에서 치사한 참매미로부터 분리되었다. 분생자병속 형성 유도 배지는 현미와 누에번데기를 원료로 하여 90:10 비율로 조제한 경우가 가장 효과적이었으며, 기타 배지 상에서는 분생자병속 발생이 관찰되지 않았다. 분생자병속은 공기 유입량이 최소일 때 형성이 효과적이었으며, 분생자병속 발생의 최적 온도는 $20^{\circ}C$, 배지 수분함량은 60% 및 조도 300 lux이었다. 또한, 증식에 활용한 종균은 액체배양균이 무성포자보다 분생자병속 형성에 더욱 효과적이었다.

This study was conducted to investigate optimal conditions for formation of synnemata from Beauveria bassiana. The strain of B. bassiana was isolated from a host of Oncotympana fuscata in Halla mountain of Jeju island. The yield of synnemata was the highest at application of brown rice (90%) and silkworm pupae (10%) media. On the other hand, the other media did not result in formation of synnemata. The highest formation of synnemata was achieved at conditions of moderate air inflow and $20^{\circ}C$. The optimal moisture and light intensity for formation of synnemata were 60% and 300 lux, respectively. In addition, inoculation of the liquid spawn resulted in higher yield than that of asexual spore.

키워드

참고문헌

  1. Basith, M. and M.F. Madelin. 1968. Studies on the production of perihelial stigmata by Cordyceps militaris in artificial culture. Can. J. Bot. 46: 473-480. https://doi.org/10.1139/b68-071
  2. Carilli, A. and G. Pacioni. 1977. Growth and sporulation of Cordyceps militaris (Linn. Ex Fr.) Link in submerged culture. Br. Mycol. Soc. 68: 237-243. https://doi.org/10.1016/S0007-1536(77)80013-2
  3. Choi, I.Y., J.S. Choi and W.H. Lee. 1999a. The production of artificial fruiting body of Paecilomyces japonica, Kor. J. Mycol. 27: 87-93.
  4. Choi, I.Y., J.S. Choi, W.H. Lee, Y.J. Yu, G.T. Joung, I.O. Ju and Y.K. Choi, 1999b. The condition of production of artificial fruiting body of Cordyceps militaris. Kor. J. Mycol. 27: 243-248.
  5. Cunningham, K. G., W. Manson, F.S. Spring and S.A. Hutchinson. 1950. Cordycepin, metabolic product isolated from cultures of Cordyceps militaris (Linn.) Link. Nature 166: 949p.
  6. Cunningham, K. G., S.A. Hutchinson, W. Manson and F.S. Spring. 1951. Cordycepin, a metabolic product from cultures of Cordyceps militaris (Linn.) Link. Part I. Isolation and characterization. J. Chem. Soc. pp. 2299-2300. https://doi.org/10.1039/jr9510002299
  7. Ha, N.G., J. H. Kim, J. H. Kang, P. D. Kang, G. B. Sung and I. P. Hong. 2005. Biological activities and cultural characteristics of and entomogenous fungus, Paecilomyces tenuipes (Peck) Samson. Kor. J. Seric. Sic. 47: 12-17.
  8. Hawksworth, D. L., P. M. Kirk, B. C. Sutton and D. N. Pegler. 1995. Dictionary of the fungi. CAB international. UK. 51p.
  9. Hubbel, H. R., E. C. Pequignot, D. H. Willis, C. Lee and R.J. Suhadolnik. 1985. Differential antiproliferative actions of 2', 5' oligo a trimer core and its cordycepin analogue on human tumor cells. Int. J. Cancer 36: 389-394.
  10. Jianzhe, Y., M. Xiaoloan, M. Qiming, Z. Yichen and W. Huaan. 1989. Icons of medicinal fungi from China. Science Press. China. 575pp.
  11. Kobayasi, Y. 1982. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans. Mycol. Soc. Japan 23: 329-364.
  12. Kobayasi, Y. and D. Shimizu. 1983. Iconography of vegetable wasps and plant worms. Hoikusha Publishing Company Ltd. Osaka. 280pp.
  13. Nam, S.H., I.Y. Jung, S.D. Ji and S.Y. Cho. 1999. Cultural condition and morphological characteristics of Paecilomyces japonica for the artificial cultivation. Kor. J. Seric, 41: 36-40.
  14. Ohmori, T., K. Tamura, S. Tsuru and K. Nomota. 1986. Antitumor activity of protein-bound polysaccharide from Cordyceps ophioglossoides in mice. Jpn. J. Cancer Res. 77: 1256-1263.
  15. Pen, X. 1995. The cultivation of C. militaris fruitbody on artificial media and the determination of SOD activity. Acta Edulis Fungi 2: 25-28.
  16. Samson, R.A, H.C. Evans and J.P. Latge. 1988. Atlas of Entomopathogenic Fungi. Springer. Heidelberg.
  17. Shah, P.A. and J.K. Pell. 2003. Entomopathogenic fungi as biological control agents. Appl. Microbiol. Biotechnol. 61: 413-423. https://doi.org/10.1007/s00253-003-1240-8

피인용 문헌

  1. Comparison of scanning electron microscopic structures and nucleotide sequences variation of ITS1, 5.8S ribosomal RNA gene and ITS2 region in three Peruvian entomopathogenic fungal isolates vol.51, pp.2, 2013, https://doi.org/10.7852/jses.2013.51.2.137