Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴

  • Bae, Yun-Ui (Department of Physiology, Yeungnam University School of Medicine) ;
  • Moon, Ik-Jae (WelGENE Inc.) ;
  • Seu, Young-Bae (School of Life Sciences & Biotechnology, Kyungpook National University) ;
  • Doh, Kyung-Oh (Department of Physiology, Yeungnam University School of Medicine)
  • Received : 2009.12.02
  • Accepted : 2010.02.23
  • Published : 2010.03.28
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Abstract

The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.

Keywords

References

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