Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Differentiation of Three Lactobacillus rhamnosus Strains (E/N, Oxy, and Pen) by SDS-PAGE and Two-Dimensional Electrophoresis of Surface-Associated Proteins

  • Jarocki, P. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ;
  • Podlesny, M. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ;
  • Wasko, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ;
  • Siuda, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin) ;
  • Targonski, Z. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
  • Received : 2009.08.03
  • Accepted : 2009.11.03
  • Published : 2010.03.31
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Abstract

SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains E/N, Oxy, and Pen, was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by the SDS-PAGE method proved to be a useful tool for strain-specific identification, which was further confirmed by 2DE analysis. Therefore, it can be used as an alternative or complementary method for both conventional and genotypic identification procedures, especially when closely related lactobacilli isolates are identified.

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References

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