DOI QR코드

DOI QR Code

Optimum Conditions for Artificial Fruiting Body Formation of Cordyceps cardinalis

  • Kim, Soo-Young (Donghae Agricultural Technology Center) ;
  • Shrestha, Bhushan (Green Energy Mission/Nepal) ;
  • Sung, Gi-Ho (National Museum of Nature and Science, Department of Botany) ;
  • Han, Sang-Kuk (Division of Forest Biodiversity, Korea National Arboretum) ;
  • Sung, Jae-Mo (Cordyceps Institute of Kangwon National University, Department of Applied Biology, Kangwon National University)
  • Received : 2010.03.08
  • Accepted : 2010.04.25
  • Published : 2010.06.30

Abstract

Stromatal fruiting bodies of Cordyceps cardinalis were successfully produced in cereals. Brown rice, German millet and standard millet produced the longest-length of stromata, followed by Chinese pearl barley, Indian millet, black rice and standard barley. Oatmeal produced the shortest-length of fruiting bodies. Supplementation of pupa and larva to the grains resulted in a slightly enhanced production of fruiting bodies; pupa showing better production than larva. 50~60 g of brown rice and 10~20 g of pupa mixed with 50~60 mL of water in 1,000 mL polypropylene (PP) bottle was found to be optimum for fruiting body production. Liquid inoculation of 15~20 mL per PP bottle produced best fruiting bodies. The optimal temperature for the formation of fruiting bodies was $25^{\circ}C$, under conditions of continuous light. Few fruiting bodies were produced under the condition of complete darkness, and the fresh weight was considerable low, compared to that of light condition.

Keywords

References

  1. Kobayasi Y. The genus Cordyceps and its allies. Sci Rept Tokyo Bunrika Daigaku Sect B 1941;5:53-260.
  2. Li CR, Nam SH, Geng DG, Fan MZ, Li ZZ. Artificial culture of seventeen Cordyceps spp. Mycosystema 2006;25:639-45.
  3. Liang Z. Anamorph of Cordyceps militaris and artificial culture of its fruitbody. Southwest China J Agric Sci 1990;3:1-6.
  4. Liu J, Liang Z, Liu A. Artificial culture of fruiting body of Cordyceps gunnii. Southwest China J Agric Sci 1990;3:6-10.
  5. Sung JM. The insects-born fungus of Korea in color. Seoul: Kyohak Publishing Co, Ltd.; 1996.
  6. Wang SF, Zhu HX, Zhu P. The preliminary cultivation and observation on the vitality of Hirsutella sinensis. Edible Fungi China 2003;22:4-6.
  7. Basith M, Madelin MF. Studies on the production of perithecial stromata by Cordyceps militaris in artificial culture. Can J Bot 1968;46:473-80. https://doi.org/10.1139/b68-071
  8. Yahagi N, Kobayashi H, Yahagi R. Cultivation of Isaria japonica. Nippon Kingakkai News 1993;20:6-8.
  9. Yahagi N, Yahagi R, Takano F, Fushiya S, Tanaka T, Murakami K, et al. Growth of ascoscarps from cultured Cordyceps militaris (L.:Fr.) Fr. and Cordyceps formicarum Kobayasi in an agar medium. Nippon Kin Gakkai Kaiho 2004;45:15-9.
  10. Yamanaka K, Inatomi S, Hanaoka M. Cultivation characteristics of Isaria japonica. Mycoscience 1998;39:43-8. https://doi.org/10.1007/BF02461577
  11. Harada Y, Akiyama N, Yamamoto K, Shirota Y. Production of Cordyceps militaris fruit body on artificially inoculated pupae of Mamestra brassicae in the laboratory. Trans Mycol Soc Jpn 1995;36:67-72.
  12. Sato H, Shimazu M. Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl Entomol Zool 2002;37:85-92. https://doi.org/10.1303/aez.2002.85
  13. Yahagi N. Cultivation of Isaria japonica and inoculation of silkworm. Touchu-Kasou 1985;5:27-30.
  14. Yu YX. Studies on artificial culture of Cordyceps sinensis. J Fungal Res 2004;2:42-6.
  15. Kanauchi A, Fukatsu T. Light-induced fruit body formation of an entomogenous fungus Paecilomyces tenuipes. Mycoscience 1999;40:349-51. https://doi.org/10.1007/BF02463879
  16. Park GB, Park GB, Shrestha B, Sung JM. Optimal medium and cultural conditions for synnemata formation of Paecilomyces tenuipes. J Mush Sci Prod 2004;2:60-8.
  17. Sung JM, Choi YS, Shrestha B, Park YJ. Investigation on artificial fruiting of Cordyceps militaris. Kor J Mycol 2002;30:6-10. https://doi.org/10.4489/KJM.2002.30.1.006
  18. Sung GH, Spatafora JW. Cordyceps cardinalis sp. nov., a new species of Cordyceps with an east Asian-eastern North American distribution. Mycologia 2004;96:658-66. https://doi.org/10.2307/3762183
  19. Lee JO, Shrestha B, Kim TW, Sung GH, Sung JM. Stable formation of fruiting body in Cordyceps bassiana. Mycobiology 2007;35:230-4. https://doi.org/10.4489/MYCO.2007.35.4.230
  20. Shrestha B, Park YJ, Han SK, Choi SK, Sung JM. Instability in in vitro fruiting of Cordyceps militaris. J Mush Sci Prod 2004;2:140-4.

Cited by

  1. Collected from Korea vol.38, pp.4, 2010, https://doi.org/10.4489/MYCO.2010.38.4.274
  2. vol.38, pp.4, 2010, https://doi.org/10.4489/MYCO.2010.38.4.282
  3. Collected in Korea vol.39, pp.2, 2011, https://doi.org/10.4489/MYCO.2011.39.2.085
  4. Mycelial Growth of <i>Paecilomyces hepiali</i> in Various Agar Media and Yield of Fruit Bodies in Rice Based Media vol.03, pp.07, 2013, https://doi.org/10.4236/aim.2013.37071
  5. NMR and GC-MS Based Metabolic Profiling and Free-Radical Scavenging Activities of Cordyceps pruinosa Mycelia Cultivated under Different Media and Light Conditions vol.9, pp.3, 2014, https://doi.org/10.1371/journal.pone.0090823
  6. Metabolomic profile and nucleoside composition of Cordyceps nidus sp. nov. (Cordycipitaceae): A new source of active compounds vol.12, pp.6, 2017, https://doi.org/10.1371/journal.pone.0179428
  7. Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies vol.103, pp.4, 2019, https://doi.org/10.1007/s00253-019-09623-3