Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Diversity of Myxobacteria in Soil Samples from Asansi and Uponeup in Korea

아산시와 우포늪 토양의 점액세균 다양성

  • Chung, Jin-Woo (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Kim, Jin-Woo (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Cho, Kyung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • 정진우 (호서대학교 생명공학과 점액세균은행) ;
  • 김진우 (호서대학교 생명공학과 점액세균은행) ;
  • 조경연 (호서대학교 생명공학과 점액세균은행)
  • Received : 2010.11.29
  • Accepted : 2010.12.29
  • Published : 2010.12.31
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Abstract

Diversity of myxobacteria in five soil samples from Asansi and Uponeup in Korea was explored by means of polymerase chain reaction (PCR) using primers that specifically bind 16S rDNA of myxobacteria. DNA sequence analysis of 76 PCR fragments containing myxobacterial 16S rDNA revealed five putative novel myxobacterial genera whose 16S rDNA sequences shared <95% sequence identity with those of the type strains. This finding indicates the presence of many uncultured and unidentified myxobacterial species in Korean soil.

점액세균의 16S rDNA에 특이적으로 부착하는 프라이머를 사용한 중합효소연쇄반응(PCR)을 통해 아산시와 우포늪에서 채취한 다섯 토양시료 내 점액세균의 다양성을 조사하였다. 점액세균의 16S rDNA을 갖는 76개 PCR 조각의 서열분석 결과, 표준균주와 95% 미만의 상동성을 보이는 5개의 신속 추정 점액세균이 관찰되어 국내 토양에 아직까지 분리되지 않은 많은 새로운 점액세균들이 존재함을 보여주었다.

Keywords

References

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