Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Indole-3-Acetic Acid by the Cyanobacterium Arthrospira platensis Strain MMG-9

  • Ahmed, Mehboob (Department of Microbiology and Molecular Genetics, University of the Punjab) ;
  • Stal, Lucas J. (Department of Marine Microbiology, Netherlands Institute of Ecology - KNAW) ;
  • Hasnain, Shahida (Department of Microbiology and Molecular Genetics, University of the Punjab)
  • Received : 2010.04.21
  • Accepted : 2010.06.14
  • Published : 2010.09.28
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Abstract

The filamentous cyanobacterium Arthrospira platensis strain MMG-9 was isolated from a rice field. The ability of this strain to synthesize the bioactive compound indole-3-acetic acid (IAA) was demonstrated. IAA was extracted from the culture of A. platensis strain MMG-9 and its identity was confirmed by thin-layer chromatography (TLC) as well as by high-performance liquid chromatography (HPLC). The IAA precursor L-tryptophan was required for IAA biosynthesis. Released IAA increased with the increase of the initial concentration of L-tryptophan in the medium and with the incubation time. A. platensis strain MMG-9 accumulated more IAA than it released into the medium. The bioactivity of the secreted IAA was shown by its effect on the formation of roots by Pisum sativum. There was a significant positive effect of the supernatant of cultures of A. platensis strain MMG-9 on the number of lateral roots of P. sativum, whereas a negative effect on root length was observed.

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References

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