Journal of Microbiology and Biotechnology

  • 제20권8호
  • /
  • Pages.1219-1225
  • /
  • 2010
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Isolation of $NH_4^+$-Tolerant Mutants of Actinobacillus succinogenes for Succinic Acid Production by Continuous Selection

  • Ye, Gui-Zi (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Jiang, Min (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Li, Jian (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Chen, Ke-Quan (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Xi, Yong-Lan (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Liu, Shu-Wen (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Wei, Ping (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology) ;
  • Ouyang, Ping-Kai (State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology)
  • 투고 : 2010.03.18
  • 심사 : 2010.05.07
  • 발행 : 2010.08.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Actinobacillus succinogenes, a representative succinicacid-producing microorganism, is seriously inhibited by ammonium ions, thereby hampering the industrial use of A. succinogenes with ammonium-ion-based materials as the pH controller. Therefore, this study isolated an ammonium-ion-tolerant mutant of A. succinogenes using a continuous-culture technique in which all the environmental factors, besides the stress (ammonium ions), were kept constant. Instead of operating the mutant-generating system as a nutrient-limited chemostat, it was used as a nutrient-unlimited system, allowing the cells to be continuously cultured at the maximum specific growth rate. The mutants were isolated on agar plates containing the acid-base indicator bromothymol blue and a high level of ammonium ions that would normally kill the parent strain by 100%. When cultured in anaerobic bottles with an ammonium ion concentration of 354 mmol/l, the mutant YZ0819 produced 40.21 g/l of succinic acid with a yield of 80.4%, whereas the parent strain NJ113 was unable to grow. When using $NH_4OH$ to buffer the culture pH in a 3.0 l stirredbioreactor, YZ0819 produced 35.15 g/l of succinic acid with a yield of 70.3%, which was 155% higher than that produced by NJ113. In addition, the morphology of YZ0819 changed in the fermentation broth, as the cells were aggregated from the beginning to the end of the fermentation. Therefore, these results indicate that YZ0819 can efficiently produce succinic acid when using $NH_4OH$ as the pH controller, and the formation of aggregates can be useful for transferring the cells from a cultivation medium for various industrial applications.

키워드

참고문헌

  1. Arnold, W. N. and J. S. Lacy. 1977. Permeability of the cell envelope and osmotic behavior in Saccharomyces cerevisiae. J. Bacteriol. 131: 564-571.
  2. Booth, I. R. 1985. Regulation of cytoplasmic pH in bacteria. Microbiol. Rev. 49: 359-378.
  3. Buurman, E. T., M. J. Teixeira de Mattos, and O. M. Neijssel. 1991. Futile cycling of ammonium ions via the high affinity potassium uptake system (Kdp) of Escherichia coil. Arch. Microbiol. 155: 391-395.
  4. Du, C., S. K. C. Lin, A. Koutinas, R. H. Wanga, M. P. Dorado, and C. Webba. 2008. A wheat biorefining strategy based on solid-state fermentation for fermentative production of succinic acid. Bioresour. Technol. 99: 8310-8315. https://doi.org/10.1016/j.biortech.2008.03.019
  5. Duncan, R. S., J. M. Withers, M. G. Wiebe, G. D. Robson, and P. J. A. Trinci. 1998. Mutants with general growth rate advantages are the predominant morphological mutants to be isolated from the Quorn${\circledR}$ production plant. Mycol. Res. 102: 221-227. https://doi.org/10.1017/S0953756297004644
  6. Jiang, M., K. Chen, Z. Liu, P. Wei, H. Ying, and H. Chang. 2010. Succinic acid production by Actinobacillus succinogenes using spent Brewer's yeast hydrolysate as a nitrogen source. Appl. Biochem. Biotechnol. 160: 244-254. https://doi.org/10.1007/s12010-009-8649-1
  7. Kleiner, D. 1985. Energy expenditure for cyclic retention of $NH_3$ $NH_{4}^{+}$ during $N_2$ fixation by Klebsiella pneumoniae. FEBS Lett. 187: 237-239. https://doi.org/10.1016/0014-5793(85)81249-7
  8. Lane, P. G., S. G. Oliver, and P. R. Butler. 1999. Analysis of a continuous-culture technique for the selection of mutants tolerant to extreme environmental stress. Biotechnol Bioeng. 65: 397-406. https://doi.org/10.1002/(SICI)1097-0290(19991120)65:4<397::AID-BIT4>3.0.CO;2-X
  9. Lee, P. C., W. G. Lee, S. Y. Lee, H. N. Chang, and Y. K. Chang. 2000. Fermentative production of succinic acid from glucose and corn steep liquor by Anaerobiospirillum succiniciproducens. Biotechnol. Bioprocess. Eng. 5: 379-381. https://doi.org/10.1007/BF02942216
  10. Li, X., Z. Zheng, Z. Wei, S. Jiang, L. Pan, and S. Weng. 2009. Screening, breeding and metabolic modulating of a strain producing succinic acid with corn straw hydrolyte. World J. Microbiol. Biotechnol. 25: 667-677. https://doi.org/10.1007/s11274-008-9936-7
  11. Liu, Y., P. Zheng, Z. Sun, Y. Ni, J. Dong, and P. Wei. 2008. Strategies of pH control and glucose-fed batch fermentation for production of succinic acid by Actinobacillus succinogenes CGMCC1593. J. Chem. Tech. Biotech. 83: 722-729. https://doi.org/10.1002/jctb.1862
  12. McKinlay, J. B., C. Vieille, and J. G. Zeikus. 2007. Prospects for a bio-based succinate industry. Appl. Microbiol. Biotechnol. 76: 727-740. https://doi.org/10.1007/s00253-007-1057-y
  13. McKinlay, J. B., J. G. Zeikus, and C. Vieille. 2005. Insights into Actinobacillus succinogenes fermentative metabolism in a chemically defined growth medium. Appl. Environ. Microbiol. 71: 6651-6656. https://doi.org/10.1128/AEM.71.11.6651-6656.2005
  14. Rudner, M. S., S. Jeremic, K. A. Petterson, D. R. Kent, K. A. Brown, M. D. Drake, W. A. Goddard, and J. D. Roberts. 2005. Intramolecular hydrogen bonding in disubstituted ethanes. A comparison of NH...O- and OH...O- hydrogen bonding through conformational analysis of 4-amino-4-oxobutanoate (succinamate) and monohydrogen 1,4-butanoate (monohydrogen succinate) anions. J. Phys. Chem. A 109: 9076-9082. https://doi.org/10.1021/jp052925c
  15. Song, H. and S. Y. Lee. 2006. Production of succinic acid by bacterial fermentation. Enzyme Microb. Technol. 39: 352-361. https://doi.org/10.1016/j.enzmictec.2005.11.043
  16. Sriram, V. and J. M. Dennis. 1999. Catalytic upgrading of fermentation-derived organic acids. Biotechnol. Prog. 15: 845-854. https://doi.org/10.1021/bp9900965
  17. Stols, L. and M. I. Donnelly. 1997. Production of succinic acid through overexpression of NAD+ dependent malic enzyme in an Escherichia coli mutant. Appl. Environ. Microbiol. 63: 695-701.
  18. Van der Werf, M. J., M. V. Guettler, M. K. Jain, and J. G. Zeikus. 1997. Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Arch. Microbiol. 167: 332-342. https://doi.org/10.1007/s002030050452
  19. Van Uden, N. 1969. Kinetics of nutrient limited growth. Annu. Rev. Microbiol. 23: 473-486. https://doi.org/10.1146/annurev.mi.23.100169.002353
  20. Wen, Z., U. Mitsuyoshi, and T. Atsuo. 2001. Genetically controlled self-aggregation of cell-surface-engineered yeast responding to glucose concentration. Appl. Environ. Microbiol. 67: 2083-2087. https://doi.org/10.1128/AEM.67.5.2083-2087.2001
  21. Willke, T. h. and K. D. Vorlop. 2004. Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Appl. Microbiol. Biotechnol. 66: 131-142. https://doi.org/10.1007/s00253-004-1733-0
  22. Zeikus, J. G., M. K. Jain, and P. Elankovan. 1999. Biotechnology of succinic acid production and markets for derived industrial products. Appl. Microbiol. Biotechnol. 51: 545-552. https://doi.org/10.1007/s002530051431

피인용 문헌

  1. Enhancement of succinic acid production by osmotic-tolerant mutant strain of Actinobacillus succinogenes vol.27, pp.12, 2010, https://doi.org/10.1007/s11274-011-0770-y
  2. Succinate production in Escherichia coli vol.7, pp.2, 2010, https://doi.org/10.1002/biot.201100061
  3. Wheat-based biorefining strategy for fermentative production and chemical transformations of succinic acid vol.6, pp.1, 2010, https://doi.org/10.1002/bbb.328
  4. Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies vol.41, pp.1, 2010, https://doi.org/10.1007/s10295-013-1346-7
  5. Coupled ARTP and ALE strategy to improve anaerobic cell growth and succinic acid production by Escherichia coli vol.91, pp.3, 2016, https://doi.org/10.1002/jctb.4633
  6. Efficient succinic acid production by engineered Escherichia coli using ammonia as neutralizer vol.91, pp.9, 2016, https://doi.org/10.1002/jctb.4828
  7. Opportunities, challenges, and future perspectives of succinic acid production by Actinobacillus succinogenes vol.102, pp.23, 2010, https://doi.org/10.1007/s00253-018-9379-5
  8. Techniques for enhancing the tolerance of industrial microbes to abiotic stresses: A review vol.67, pp.1, 2010, https://doi.org/10.1002/bab.1794