• 제목/요약/키워드: continuous-culture technique

검색결과 42건 처리시간 0.026초

메타놀자화균의 연속배양에 의한 균체생산의 온-라인 적응최적화 (Adaptive On-line Optimization of Cellular Productivity of Continuous Methylotroph Culture)

  • 이형춘;박정오
    • 한국식품영양학회지
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    • 제1권2호
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    • pp.31-36
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    • 1988
  • An adaptive on-line optimization method has been applied to test the ability to maximize the cellular productivity of a continuous methylotroph culture system which was simulated by a variable yield Monod-type model. Optimum dilution rate and productivity were successively obtained and maintained at all times by the algorithm that utilizes steepest descent technique as optimization method and recursive least-square method with forgetting factor as dynamic model identification.

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모범사례기업 조직문화가 작은기업의 기술혁신에 미치는 효과 (Best Practice Organizations Cultural Effects on Innovation in Small Company)

  • 정경식
    • 산업융합연구
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    • 제13권3호
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    • pp.41-56
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    • 2015
  • People have relatively continuous and stable characteristic and it can help understand personal attitude and behavior. As individual has individual personality and society has culture, in case of organization, there is organization culture that means unique cultural characteristic. And as if we need to know culture of that society to understand individual, we need to know organization culture of that organization to understand any organization. The reason why organization culture is acknowledged as modern theory of business management is-modern company has proposed management innovation, organization activation, company transformation, and company reprogramming as management strategy to cope actively with various and quickly changing environment that modern company face-that organization culture and management innovation, that is, the concept of new business management is highlighted for means and tools to solve such problems. Seeing contemporary situation, although organization culture of small company is being improved by management innovation, cause and effect that organization culture affect technique innovation still insufficient. The meaning of this research is, through a good example of organization culture, providing reason that efficient organization culture affect technique innovation of small company(medium and small company) and making people understand why this effect occur. Through this, I want to provide strategy and policy implication of company.

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Production of 3-Ketosteroid-delta-1-Dehydrogenase by a Two-stage Continuous Culture

  • Ryu, D.Y.;Lee, B.K.;Thoma, R.W.
    • 한국미생물·생명공학회지
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    • 제2권1호
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    • pp.29-35
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    • 1974
  • We have studied the applicability of the principles and inherent advantages of the two-stage dontinuous uclture technique to an enzyme process for the purpose of improving and optimizing the productivity of 3-ketosteroid-delta-1-dehydrogenase. By using a two-stage continuous culture system, the growth st ageand enzyme produdtion stage are separated. In each stage an optimal set of toperaing conditions was determined, and this was tested for feasibility for the period of 10 days. During this period, at least 70% of the maximum enzyme productivity could be maintained. The important design parameters studied are: (1) optimal specific growth rate in the first stage which corresponds to the maximal cell productivity, (2) the optimal dilution rate in the second stage which in turn determines the size of second stage fermentor and the mean residence time of cells in the second stage, (3) cell concentration in both stages, add (4) the specific enzyme productivity and enzyme productivity of the second stage. In addition, by using two-stage continuous culture system we have been able to reduce or eliminate the effect of catabolite repression due to high medium concentration and the adverse effect of the solvent used to dissolve the inducer. We have found the balance between the opposing effects of induction and repression in the second stage judging from the observation that the enzyme productivity goes through a maximum.

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Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

  • Selvakumar, Gopal;Kim, Kiyoon;Walitang, Denver;Chanratana, Mak;Kang, Yeongyeong;Chung, Bongnam;Sa, Tongmin
    • 한국토양비료학회지
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    • 제49권5호
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    • pp.608-613
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    • 2016
  • Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

페놀분해세균의 분리 및 생물학적 처리 특성 (Characterization of Biological Treatment by an Isolated Phenol-Degrading Bacterium)

  • 송형의;김진욱
    • 한국환경보건학회지
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    • 제24권3호
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    • pp.54-62
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    • 1998
  • 20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.

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Isolation of $NH_4^+$-Tolerant Mutants of Actinobacillus succinogenes for Succinic Acid Production by Continuous Selection

  • Ye, Gui-Zi;Jiang, Min;Li, Jian;Chen, Ke-Quan;Xi, Yong-Lan;Liu, Shu-Wen;Wei, Ping;Ouyang, Ping-Kai
    • Journal of Microbiology and Biotechnology
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    • 제20권8호
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    • pp.1219-1225
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    • 2010
  • Actinobacillus succinogenes, a representative succinicacid-producing microorganism, is seriously inhibited by ammonium ions, thereby hampering the industrial use of A. succinogenes with ammonium-ion-based materials as the pH controller. Therefore, this study isolated an ammonium-ion-tolerant mutant of A. succinogenes using a continuous-culture technique in which all the environmental factors, besides the stress (ammonium ions), were kept constant. Instead of operating the mutant-generating system as a nutrient-limited chemostat, it was used as a nutrient-unlimited system, allowing the cells to be continuously cultured at the maximum specific growth rate. The mutants were isolated on agar plates containing the acid-base indicator bromothymol blue and a high level of ammonium ions that would normally kill the parent strain by 100%. When cultured in anaerobic bottles with an ammonium ion concentration of 354 mmol/l, the mutant YZ0819 produced 40.21 g/l of succinic acid with a yield of 80.4%, whereas the parent strain NJ113 was unable to grow. When using $NH_4OH$ to buffer the culture pH in a 3.0 l stirredbioreactor, YZ0819 produced 35.15 g/l of succinic acid with a yield of 70.3%, which was 155% higher than that produced by NJ113. In addition, the morphology of YZ0819 changed in the fermentation broth, as the cells were aggregated from the beginning to the end of the fermentation. Therefore, these results indicate that YZ0819 can efficiently produce succinic acid when using $NH_4OH$ as the pH controller, and the formation of aggregates can be useful for transferring the cells from a cultivation medium for various industrial applications.

Islamic Leadership, Islamic Work Culture, and Employee Performance: The Mediating Role of Work Motivation and Job Satisfaction

  • ASTUTI, Sih Darmi;SHODIKIN, Ali;UD-DIN, Maaz
    • The Journal of Asian Finance, Economics and Business
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    • 제7권11호
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    • pp.1059-1068
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    • 2020
  • This study aims to examine the influence of Islamic leadership and Islamic work culture on employee performance through work motivation and job satisfaction. This study was conducted at Universitas Islam Negeri (UIN) Walisongo Semarang, Indonesia, because so far, there is still very limited research on the relationships between Islamic leadership, work culture, and work motivation in educational personnel performance. The educational personnel performance is expected to increase at UIN Walisongo Semarang with continuous efforts to improve to survive and develop as much as possible. The population of this study was all educational personnel at UIN Walisongo Semarang, Indonesia. Of 186 people, only 127 people were involved in this study. The research method used was a survey method using primary data collected through questionnaires. This study's analysis technique employed multiple linear regression. The results uncovered that, out of the eight hypotheses proposed, six hypotheses were accepted. Islamic leadership and Islamic work culture were found to have no direct effect on employee performance. Besides, work motivation and job satisfaction became essential factors mediating Islamic leadership and Islamic work culture on employee performance. Spiritual values in leadership inspire employees to build the organization's vision and create job satisfaction, which in turn impacted employees' performance.

연속 다중 이미지 기반 3D 생성 모델 보정 기술 개발 (Corrected 3D Reconstruction Based on Continuous Image Sets)

  • 김태연;조동식
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2022년도 추계학술대회
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    • pp.374-375
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    • 2022
  • 최근, 메타버스 서비스를 통해 시간적, 공간적 제약에서 벗어나 원격지와 의사 소통을 자연스럽게 진행하는데 많이 사용하고 있다. 이러한 메타버스 콘텐츠 제작을 위해서는 실제 공간의 데이터를 기반으로 3D 모델을 복원하고, 합성할 필요가 있다. 본 논문에서는 다수의 카메라를 이용한 연속된 이미지를 기반으로 3D 생성 복원 모델을 제작하고, 복원된 3D 모델을 보정할 수 있는 기법을 제시한다. 이를 위해 오프라인으로 다중 카메라 셋업을 수행하고, 여러 각도에서 얻어진 이미지를 통해 만들어진 3D 모델에 대해 오류를 분석한 뒤 이미지 프레임 간의 매칭 기법으로 보정을 수행 하였다. 본 논문에서 제시한 움직이는 3D 물체 복원 방법을 통해 문화, 관광, 의료 등 다양한 서비스 분야에 3D 복원 데이터를 활용할 수 있을 것으로 본다.

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혐기성세균모델을 이용한 봉함제(Sealer)의 미세누출에 관한 연구 (LEAKAGE EVALUATION OF SEVERAL SEALERS USING ANAEROBIC BACTERIAL LEAKAGE MODEL)

  • 배용규;오태석;윤수한
    • Restorative Dentistry and Endodontics
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    • 제25권2호
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    • pp.235-242
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    • 2000
  • The purpose of this in vitro study was to evaluate the sealing ability of three sealers(Sealapex, Pulp canal sealer, AH26) used with continuous wave method using an anaerobic bacterial leakage model. 53 extracted human teeth with straight and single canals were prepared with crown-down pressureless technique using .04, .06 taper Profile(Maillefer, Swiss). Master apical file was maintained as #35 K-file. All canals of the experimental teeth were obturated with continuous wave method using System B(Analytic technology, U.S.A.) The teeth were randomly divided into three experimental groups of 15 and two control groups of 4. Experimental group 1 was obturated with Sealapex and group 2 with Pulp canal sealer, and group 3 with AH26. A dual chamber anaerobic bacterial leakage model was assembled. Brain heart infusion with yeast extract, hemin, menadion, and the chromogenic indicator bromocresol purple was used as the culture broth for Fusobacterium nucleatum(VPI 10197), The specimens were incubated in anaerobic chamber at $37^{\circ}C$ and were observed every 2 to 3 clays, The coronal leakage was evaluated through the color change of culture broth in lower chamber for 60 days. The results were as follows: 1. The incidence of bacterial leakage in group 1 (Sealapex group was 80%, 53% in group 2 (Pulp canal sealer), 27% in group 3 (AH26). 2. There were statistically significant differences in leakage scores between group 1 and group 2, and between group 1 and group 3, respectively. (P<0.05) 3. There was no significantly difference in leakage score between group 2 and group 3. (P>0 05)

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한국과 일본의 장인(匠人)정신 비교 연구 (The Comparative Study of The View about the Craftsmanship of Korean and Japanese)

  • 정수현
    • 비교문화연구
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    • 제35권
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    • pp.215-235
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    • 2014
  • The purpose of this paper look at the basis of the cultural differences of Korean and Japan by comparing the craftsmanship. Korean craftsmanship and master craftsman making traditional handcrafts and artwork would die out at a critical moment. The craftsmanship and system could not be established because of discrimination in the traditional four classes of society( scholars, farmers, artisans and tradesmen), invasions of other countries and discontinuity of tradition from modernization. On the contrast, in Japan, it was possible to establish traditional handcrafts and artworks, technique and craftsmanship with apprentice system and various social privilege and treatment even in the same traditional four classes of society ( scholars, farmers, artisans and tradesmen). It was common to pass down the family business for a hundreds of years with self respect between Japanese master craftsmen and lots of people succeed the family business will now. Japanese craftsmanship is originated from 'worship to god' Medieval Japanese believed that the thing changes human mind, artistic talent, masterpieces and faith were connected to sacred power. Therefore, master craftsmen and their works were also made by the power of god. In this context, craftsmen were protected and treated in socially and nationally. It is that the social treatments and supports in national level and their own pride and continuous training of techniques and abilities as the main driving force to maintain the craftsmanship in Japan. Korea has the sale level of competitive tradition and technique in comparison any other countries. Japanese craftsmanship study will be a critical data in the protection and maintenance of Korean traditional and technique.