Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Cellulolytic and Xylanolytic Enzyme Complex from an Alkalothermoanaerobacterium, Tepidimicrobium xylanilyticum BT14

  • Phitsuwan, Paripok (Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Tachaapaikoon, Chakrit (Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi) ;
  • Kosugi, Akihiko (Post-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences) ;
  • Mori, Yutaka (Post-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences) ;
  • Kyu, Khin Lay (Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi,) ;
  • Ratanakhanokchai, Khanok (Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi)
  • Received : 2009.11.07
  • Accepted : 2010.01.13
  • Published : 2010.05.28
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Abstract

A cellulolytic and xylanolytic enzyme complex-producing alkalothermoanaerobacterium strain, Tepidimicrobium xylanilyticum BT14, is described. The cell was Grampositive, rod-shaped, and endospore-forming. Based on 16S rRNA gene analysis and various lines of biochemical and physiological properties, the strain BT14 is a new member of the genus Tepidimicrobium. The strain BT14 cells had the ability to bind to Avicel, xylan, and corn hull. The pH and temperature optima for growth were 9.0 and $60^{\circ}C$, respectively. The strain BT14 was able to use a variety of carbon sources. When the bacterium was grown on corn hulls under an anaerobic condition, a cellulolytic and xylanolytic enzyme complex was produced. Crude enzyme containing cellulase and xylanase of the strain BT14 was active in broad ranges of pH and temperature. The optimum conditions for cellulase and xylanase activities were pH 8.0 and 9.0 at $60^{\circ}C$, respectively. The crude enzyme had the ability to bind to Avicel and xylan. The analysis of native-PAGE and native-zymograms indicated the cellulosebinding protein showing both cellulase and xylanase activities, whereas SDS-PAGE zymograms showed 4 bands of cellulases and 5 bands of xylanases. Evidence of a cohesinlike amino acid sequence seemed to indicate that the protein complex shared a direct relationship with the cellulosome of Clostridium thermocellum. The crude enzyme from the strain BT14 showed effective degradation of plant biomass. When grown on corn hulls at pH 9.0 and $60^{\circ}C$ under anaerobic conditions, the strain BT14 produced ethanol and acetate as the main fermentation products.

Keywords

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