Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Neuroprotective Effects of a Novel Peptide Purified from Venison Protein

  • Kim, Eun-Kyung (Department of Biotechnology, College of Medicine Konkuk University) ;
  • Lee, Seung-Jae (Department of Biotechnology College of Medicine Konkuk University) ;
  • Moon, Sang-Ho (Korean Nokyong Research Center, College of Medicine Konkuk University) ;
  • Jeon, Byong-Tae (Korean Nokyong Research Center, College of Medicine Konkuk University) ;
  • Kim, Bo-Kyung (Department of Physiology, College of Medicine Konkuk University) ;
  • Park, Tae-Kyu (Department of Biotechnology, College of Medicine Konkuk University) ;
  • Han, Ji-Sook (Department of Food Science and Nutrition, Pusan National University) ;
  • Park, Pyo-Jam (Department of Biotechnology, College of Medicine Konkuk University)
  • Received : 2009.09.21
  • Accepted : 2009.11.25
  • Published : 2010.04.28
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Abstract

A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel, and high-performance liquid chromatography. The molecular mass of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against $H_2O_2$-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA), and cell death against $H_2O_2$-induced neuronal cell damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment. The present results indicate that APVPH I substantially contributes to antioxidative properties in neuronal cells.

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References

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