DOI QR코드

DOI QR Code

Molecular Diversity of Fungal Endophytes Isolated from Garcinia mangostana and Garcinia parvifolia

  • Sim, Jiun-Horng (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia) ;
  • Khoo, Chai-Hoon (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia) ;
  • Lee, Learn-Han (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia) ;
  • Cheah, Yoke-Kqueen (Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia)
  • 투고 : 2009.09.18
  • 심사 : 2009.12.06
  • 발행 : 2010.04.28

초록

Garcinia is commonly found in Malaysia, but limited information is available regarding endophytic fungi associated with this plant. In this study, 24 endophytic fungi were successfully recovered from different parts of two Garcinia species. Characterization of endophytic fungi was performed based on the conserved internal transcribed spacer (ITS) region sequence analysis and the antimicrobial properties. Results revealed that fruits of the plant appeared to be the highest inhabitation site (38%) as compared with others. Glomerella sp., Guignardia sp., and Phomopsis sp. appeared to be the predominant endophytic fungi group in Garcinia mangostana and Garcinia parvifolia. Phylogenetic relationships of the isolated endophytic fungi were estimated from the sequences of the ITS region. On the other hand, antibacterial screening showed 11 of the isolates possessed positive response towards pathogenic and nonpathogenic bacteria. However, there was no direct association between certain antibacterial properties with the specific genus observed.

키워드

참고문헌

  1. Atmosukarto, I., U. Castillo, W. M. Hess, J. Sear, and G. Strobel. 2005. Isolation and characterization of Muscodor albus I-41.3s, a volatile antibiotic producing fungus. Plant Sci. 169: 854-861. https://doi.org/10.1016/j.plantsci.2005.06.002
  2. Brayford, D. 1990. Variation in Phomopsis isolates from Ulmus species in the British Isles and Italy. Mycol. Res. 94: 691-697. https://doi.org/10.1016/S0953-7562(09)80670-9
  3. Crozier, J., S. E. Thomas, M. C. Aime, H. C. Evans, and K. A. Holmes. 2006. Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol. 55: 783-791. https://doi.org/10.1111/j.1365-3059.2006.01446.x
  4. Eichner, C. A., R. W. Erb, K. N. Timmis, and I. Wagner-Dobler. 1999. Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community. Appl. Environ. Microbiol. 65: 102-109.
  5. Enrico, G. A. L. and A. V. D. A. A. Hubertus. 1995. Taxonomy, sexuality and mating types of Diaporthe adunca. Mycol. Res. 99: 1409-1416. https://doi.org/10.1016/S0953-7562(09)80786-7
  6. Ezra, D., W. M. Hess, and G. A. Strobel. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology 150: 4023-4031. https://doi.org/10.1099/mic.0.27334-0
  7. Fei, Y., H. Ting, J. Z. Wu, B. K. Huang, and L. P. Qin. 2009. Antifungal secondary metabolites from endophytic Verticillium sp. Biochem. Syst. Ecol. 37: 162-165. https://doi.org/10.1016/j.bse.2009.03.008
  8. Ganley, R. J., S. J. Brunsfeld, and G. Newcombe. 2004. A community of unknown, endophytic fungi in western white pine. Proc. Natl. Acad. Sci. U.S.A. 101: 10107-10112. https://doi.org/10.1073/pnas.0401513101
  9. Goes-Neto, A., C. Loguercio-Leite, and R. T. Guerrero. 2005. DNA extraction from frozen field-collected and dehydrated herbarium fungal basidiomata: Performances of SDS and CTAB-based methods. Biotemas 18: 19-32.
  10. Hawksworth, D. C. and A. Y. Rossman. 1987. Where are the undescribed fungi? Phytopathology 87: 888-891.
  11. Hershkovitz, M. A., E. A. Zimmer, and W. J. Hans. 1999. Ribosomal DNA sequences and angiosperm systematics, pp. 268-326. In P. M. Hollingsworth, R. M. Bateman, and R. J. Gornall (eds.). Systematics and Plant Evolution. Taylor and Francis, London.
  12. Kim, S., D.-S. Shin, T. Lee, and K.-B. Oh. 2004. Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. J. Nat. Prod. 67: 448-450. https://doi.org/10.1021/np030384h
  13. Li, Y. S., J. Y. Liu, Y. M. Ma, and R. X. Tan. 2005. Anti-Helicobacter pylori substances from endophytic fungal cultures. World J. Microbiol. Biotechnol. 21: 553-558. https://doi.org/10.1007/s11274-004-3273-2
  14. Liang-Dong, G., H. Guo-Rui, and W. Yu. 2008. Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis (Pinaceae) in the Dongling Mountains, Beijing. J. Integr. Plant Biol. 50: 997-1003. https://doi.org/10.1111/j.1744-7909.2008.00394.x
  15. Moongkarndi, P., N. Kosem, O. Luanratana, S. Jongsomboonkusol, and N. Pongpan. 2004. Antiproliferative activity of Thai medicinal plants extracts on human breast adenocarcinoma cell line. Fitoterapia 75: 375-377. https://doi.org/10.1016/j.fitote.2004.01.010
  16. Mungo, C. M., A. M. Emechebe, and D. A. Florini. 1998. Isolation of Sphaceloma sp. from four cowpea plant parts using eight media. Crop Prot. 17: 341-343. https://doi.org/10.1016/S0261-2194(98)00025-8
  17. Nakatani, K., M. Atsumi, T. Arakawa, K. Oosawa, S. Shimura, N. Nakahata, and Y. Ohizumi. 2002. Inhibitions of histamine release and prostaglandin E2 synthesis by mangosteen, a Thai medicinal plant. Biol. Pharm. Bull. 25: 1137-1141. https://doi.org/10.1248/bpb.25.1137
  18. Zin, N. M., N. I. M. Sarmin, N. Ghadin, D. F. Basri, N. M. Sidik, W. M. Hess, and G. A. Strobel. 2007. Bioactive endophytic streptomycetes from the Malay Peninsula. FEMS Microbiol. Lett. 274: 83-88. https://doi.org/10.1111/j.1574-6968.2007.00819.x
  19. Petrini, O. and P. J. Fisher. 1988. A comparative study of fungal endophytes in xylem and whole stem of Pinus sylvestris and Fagus sylvatica. Trans. Br. Mycol. Soc. 91: 233-238. https://doi.org/10.1016/S0007-1536(88)80210-9
  20. Phongpaichit, S., J. Nikom, N. Rungjindamai, J. Sakayaroj, N. Hutadilok-Towatana, V. Rukachaisirikul, and K. Kirtikara. 2007. Biological activities of extract from endophytic fungi isolated from Garcinia plants. FEMS Immunol. Med. Microbiol. 56: 517-525.
  21. Phongpaichit, S., N. Rungjindamai, V. Rukachaisirikul, and J. Sakayaroj. 2006. Antimicrobial activity in cultures of endophytic fungi isolated from Garcinia species. FEMS Immunol. Med. Microbiol. 48: 367-372. https://doi.org/10.1111/j.1574-695X.2006.00155.x
  22. Qi, F. H., T. Z. Jing, Z. X. Wang, and Y. G. Zhan. 2009. Fungal endophyte from Acer ginnala Maxim: Isolation, identification and their yield of gallic acid. Lett. Appl. Microbiol. doi:10.1111/ j.1472-765X.2009.02626.x (In Press).
  23. Stinson, M., D. Ezra, W. M. Hess, J. Sears, and G. Strobel. 2003. An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compound. Plant Sci. 165: 913-922. https://doi.org/10.1016/S0168-9452(03)00299-1
  24. Strobel, G. A. 2003. Endophytes as sources of bioactive products. Microbes Infect. 5: 535-544. https://doi.org/10.1016/S1286-4579(03)00073-X
  25. Tefera, T. and S. Vidal. 2009. Effect of inoculated method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana. Biocontrol 54: 663-669. https://doi.org/10.1007/s10526-009-9216-y
  26. White, T. F., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, F. S. Sninsky, and T. T. White (eds.). PCR Protocol: A Guide to Methods and Applications. Academic Press, San Diego.
  27. Williamson, P. M., A. S. Highet, W. Gams, K. Sivasithamparam, and W. A. Cowling. 1994. Diaporthe toxica sp. nov., the cause of lupinosis in sheep. Mycol. Res. 98: 1364-1368. https://doi.org/10.1016/S0953-7562(09)81064-2
  28. Wilson, D. 1995. Endophytes - the evolution of a term and clarification of its use and definition. Oikos 73: 274-276. https://doi.org/10.2307/3545919
  29. Won, H. and S. S. Renner. 2005. The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Mol. Phylogenet. Evol. 36: 581-597. https://doi.org/10.1016/j.ympev.2005.03.011

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