Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

In Vitro Mass Propagation and Economic Effects of Bioreactor Culture in Ever-bearing Strawberry 'Goha'

Bioreactor를 이용한 사계성 딸기 기내대량증식과 경제성

  • Lee, Jong-Nam (Highland Agricultural Research Center, National Institute of Crop Science) ;
  • Kim, Hye-Jin (Highland Agricultural Research Center, National Institute of Crop Science) ;
  • Kim, Ki-Deog (Highland Agricultural Research Center, National Institute of Crop Science) ;
  • Kwon, Young-Seok (Highland Agricultural Research Center, National Institute of Crop Science) ;
  • Im, Ju-Sung (Highland Agricultural Research Center, National Institute of Crop Science) ;
  • Lim, Hak-Tae (Department of Bio-Health Technology, Kangwon National University) ;
  • Yeoung, Young-Rok (Department of Plant Science, Kangneung-Wonju National University)
  • 이종남 (국립식량과학원 고령지농업연구센터) ;
  • 김혜진 (국립식량과학원 고령지농업연구센터) ;
  • 김기덕 (국립식량과학원 고령지농업연구센터) ;
  • 권영석 (국립식량과학원 고령지농업연구센터) ;
  • 임주성 (국립식량과학원 고령지농업연구센터) ;
  • 임학태 (강원대학교 생명건강공학전공) ;
  • 용영록 (강릉원주대학교 식물생명과학과)
  • Received : 2010.07.23
  • Accepted : 2010.08.18
  • Published : 2010.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to compare various culture methods and evaluate economic feasibility of each method for mass propagation of new ever-bearing strawberry 'Goha'. Four different methods such as semi-solid culture, solid culture, liquid suspension culture and bioreactor culture were compared. The solid culture and bioreactor culture showed the shortest and longest root length, such as 3.6 cm and 8.3 cm, respectively. Fresh weights of plants cultured in bioreactor were 2,261 mg, which were heavier than those of cultures. Dry weights of plants cultured in bioreactor were the heavier compared to those in other cultures. The number of axillary bud developed in bioreactor was seven, but axillary bud was not developed in other cultures. Production cost through bioreactor culture was calculated to be 303 won per plant which was 542 won less than that of solid culture. As a result, we found that the bioreactor culture was the most cost effective culture method for in vitro mass propagation in new ever-bearing strawberry 'Goha'.

본 실험은 여름딸기 무병묘 대량증식을 위해 bioreactor배양의 증식 및 경제성 효과를 비교하고자 실시하였다. 배양 방법은 반고체, 고체, 현탁배양 및 bioreactor 배양 등 4가지 방법을 이용하였다. 배양 6주 후, 식물체의 초장은 고체배양이 3.6cm로 가장 짧았으며, bioreactor 배양이 8.3cm로 가장 길었다. 생체중과 건물중은 bioreactor 배양이 2,261mg과 525mg으로 다른 배양방법에 비하여 가장 무거웠다. 액아는 반고체, 고체 및 현탁배양은 거의 발생하지 않았으나, bioreactor 배양은 주당 7개의 액아가 발생하였다. 경제성 분석 결과 기본식물 생산 시 bioreactor배양이 303원/주으로 고체배양의 845원/주보다 542원/주 적었다. 따라서 딸기 무병묘 생산 시 bioreactor배양이 대량증식 및 경제적인 면에서 효율적이었다.

Keywords

References

  1. Akita, M. 2000. Bioreactor culture of plant organs In: Spier, R.E.; B. Griffiths and A.H. Scragg (Eds.) The Encyclopedia of Cell Technology. John Wiley & Sons, Inc., New York, p.129-138.
  2. Akita, M. and S. Takayama. 1994a. Induction and development of potato tubers in a jar fermentor. Plant Cell Tissue Org. Cult. 36:177-182. https://doi.org/10.1007/BF00037717
  3. Akita, M. and S. Takayama. 1994b. Stimulation of potato (Solanum tuberosum L.) tuberization by semi-continuous liquid medium surface level control. Plant Cell Rep. 13:184-187.
  4. Ammirato, P. 1983. Handbook of plant cell cultures. p. 82-123 In: D.A. Evans., W.R. Sharp, P.V. Ammirato and Y. Yamada (eds.). Embryogenesis. Mac Millan, New York.
  5. Bajaj, Y.P.S. 1986. Biotechnology in Agriculture and Forestry. p. 1-2. In: Y.P.S. Bajaj. (ed.). Biotechnology of tree improvement for rapid propagation and biomass energy production. Springer, Berlin Heidelberg, New York.
  6. Bajaj, Y.P.S., M. Furmanova, and O. Olszowska. 1988. Biotechnology in agriculture and forestry. p. 60-103. In: Y.P.S. Bajaj. (ed). Biotechnology of the micropropagation of medicinal and aromatic plants. Springer, Berlin, Heidelberg, New York.
  7. Boxus, P.H. 1974. The production of strawberry plants by in vitro micropropagation. J. Hort. Sci. 49:209-210.
  8. Boxus, P.H. 1976. Rapid production of virus-free strawberry by 'in vitro' culture. Acta Hort. 66:35-38.
  9. Boxus, P.H., M. Quoirin, and J.M. Laine. 1977. Applied and fundamental aspects of plant cell, tissue and organ culture. p. 130-143. In: J. Reinert. and Y.P.S. Bajaj (eds.). Large scale propagation of strawberry plants from tissue culture. Springer- Verlag, Heidelberg.
  10. Damiano, C. 1980. Proceedings of the conference on nursery production of fruit plants through tissue culture. Applications and feasibility. p. 93-101. In: Planning and building a tissue culture laboratory. USDA.
  11. Hanhineva, K. and S.O. Kärenlampi. 2007. Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR. BMC Biotechnology. 7:11. https://doi.org/10.1186/1472-6750-7-11
  12. Manosh, K.B., M. Hossain, and R. Islam. 2007. Virus free plantlets production of strawberry through meristem culture. World Journal of Agricultural Sciences. 3:757-763.
  13. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473-479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Takayama, S. 2000. Biotechnology in Agriculture and Forestry. Vol.17. p. 495-515. In: Y.P.S. Bajaj. (ed.). Mass propagation of plants through shake and bioreactor culture technique. Springer-Verlag, Berlin.
  15. Takayama, S. 2002. Practical aspects of bioreactor application in mass propagation of plants. Abst. 1st Int. Symp. Liquid systems for in vitro mass propagation of plants. Norway, p. 60-62.
  16. Takayama, S. and M. Akita. 1994. The types of bioreactors used for shoots and embryos. Plant Cell Tissue Org. Cult. 39:147- 156. https://doi.org/10.1007/BF00033922
  17. Takayama, S. and M. Akita. 1998. Bioreactor techniques for large-scale culture of plant propagules. Adv. Hort. Sci. 12:93-100.
  18. Takayama, S. and M.Akita. 2005. Liquid culture systems for in vitro plant propagation. p. 61-78. In: A.K. Hvoslef-Eide and W. Preil (eds). Practical aspects of bioreactor application in mass propagation of plants. Springer. Netherlands.
  19. Takayama, S. and M. Akita. 2008. Plant tissue culture engineering. p. 83-100. In: S. Dutta Gupta and Y. Ibaraki (eds.). Bioengineering aspects of bioreactor application in plant propagation. Springer-Verlag, Berlin.
  20. Takayama, S., T. Amo, M. Fukano, K. Nakazawa, and K. Oosawa. 1985. Mass propagation of strawberries by jar fermentor culture. (2) Studies on the optimum conditions in a liquid medium and the establishment of mass propagation scheme using a jar fermentor. Abst. 1985 Spring meeting of Japanese society for horticultural science. Jpn. Soc. Hort. Sci. Tokyo. p. 210-221.
  21. Takayama, S. and M. Misawa. 1981. Mass propagation of Begonia hiemalis plantlets by shake culture. Plant Cell Physiol. 22:461-468.
  22. Takayama, S., Y. Arima, and M. Akita. 1986. Mass propagation of plants by fermentor culture techniques. In: Abst. 6th International congress of plant tissue and cell culture, Int. Assoc. Plant Tissue Cult. Univ. of Minnesota. p. 449.