Antioxidant Effect according to Extraction Method in Extracts of Dendranthema zawadskii var. yezoense and Cosmos bipinnatus

추출 방법에 따른 남구절초와 코스모스 추출물의 항산화 효과

  • Woo, Jeong-Hyang (Department of Horticultural Science, Chungbuk National University) ;
  • Shin, So-Lim (Department of Horticultural Science, Chungbuk National University) ;
  • Chang, Young-Deug (Department of Horticultural Science, Chungbuk National University) ;
  • Lee, Cheol-Hee (Department of Horticultural Science, Chungbuk National University)
  • Received : 2009.04.24
  • Accepted : 2009.08.10
  • Published : 2010.06.30


Top parts of $Dendranthema$ $zawaskii$ var. $yezoense$ (DZY) and $Cosmos$ $bipinnatus$ (CB) are believed to contain substances with antioxidant effects and activity. Present experiments were undertaken to investigate an adequate and efficient extraction method which ensures the highest yield of antioxidant components. Extraction yield was 1.11-1.92 times higher by ultrasonic method than other methods in both species. By 45 minute ultrasonic extraction, total polyphenol contents reached the highest level, $80.70mg{\cdot}g^{-1}$ tannic acid equiv./DW in $Cosmos$ $bipinnatus$ and total flavonoid contents $50.41mg{\cdot}g^{-1}$ naringin equiv./DW in DZY. DPPH radical scavenging activity was almost the same among extraction methods or slightly higher in reflux extraction. The highest DPPH radical scavenging was observed by reflux extract in CB ($RC_{50}=0.135mg{\cdot}mL^{-1}$). ABTS radical scavenging activity was the highest by 15 minute ultrasonic wave in DZY and CB, $RC_{50}=0.159mg{\cdot}mL^{-1}$ and $RC_{50}=0.139mg{\cdot}mL^{-1}$, respectively. High ferrous ion chelating effects were obtained by 30-minute ultrasonic wave in DZY ($RC_{50}=0.803mg{\cdot}mL^{-1}$). Extracts of top parts of DZY obtained by reflux method showed the highest inhibition effect against peroxidation of linoleic acid, and extract of CB obtained by ultrasonic for 45 minutes showed the highest inhibition effect. In conclusion, optimum extraction method should be adjusted according to plant species and antioxidant substances.

항산화 활성이 우수할 것으로 기대되는 남구절초($Dendranthema$ $zawaskii$ var. $yezoense$)와 코스모스($Cosmos$ $bipinnatus$) 지상부의 항산화 물질 함량 및 항산화 활성을 증가시킬 수 있는 효율적인 추출방법을 개발하기 위하여 본 연구를 시행하였다. 추출수율은 두 종 모두 초음파추출물에서 우수하였으며, 환류냉각추출물보다 1.11-1.92배 증가되었다. 총 폴리페놀과 총 플라보노이드의 함량 또한 초음파추출물에서 우수하였다. 총 폴리페놀은 함량은 45분 동안 초음파추출한 코스모스에서 $80.70mg{\cdot}g^{-1}$ DW로 가장 높았고, 총 플라보노이드 함량은 45분 동안 초음파추출한 남구절초에서 $50.41mg{\cdot}g^{-1}$ DW로 가장 높았다. DPPH radical 소거능은 추출방법에 따른 차이가 없거나 환류냉각추출물이 다소 우수한 경향을 보였으며, 환류냉각추출한 코스모스 지상부 추출물의 소거능($RC_{50}=0.135mg{\cdot}mL^{-1}$)이 가장 우수하였다. ABTS radical 소거능은 남구절초와 코스모스 모두 초음파 15분 추출물에서 가장 우수하였다(각각 $RC_{50}$=0.159, $0.139mg{\cdot}mL^{-1}$). $Fe^{2+}$ chelating효과는 30분 동안 초음파추출한 남구절초 지상부에서 가장 높았다($RC_{50}=0.803mg{\cdot}mL^{-1}$). 남구절초 지상부 추출물의 지질과산화 억제활성은 환류냉각추출물에서 가장 우수하였으나, 코스모스 지상부 추출물의 지질과산화 억제활성은 45분 동안 초음파추출한 처리구에서 가장 높았다. 연구 결과, 식물 종과 목표로 하는 항산화 효과에 따라 추출방법을 달리해야 할 것으로 생각되었다.



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