오리엔탈 나리 조직배양구의 순화, 비대를 위한 적정 재식밀도 및 깊이

Proper Planting Density and Depth for Acclimation of Tissue-cultured Bulblets in Lilium Oriental Hybrids

  • 고재영 (강원도농업기술원 원예연구과) ;
  • 최강준 (강원도농업기술원 원예연구과) ;
  • 홍대기 (강원도농업기술원 원예연구과) ;
  • 이혜경 (국립원예특작과학원 화훼과)
  • Ko, Jae-Young (Horticulture Research Division, Gangwon Provincial Agricultural Research and Extension Services) ;
  • Choi, Kang-Joon (Horticulture Research Division, Gangwon Provincial Agricultural Research and Extension Services) ;
  • Hong, Dae-Ki (Horticulture Research Division, Gangwon Provincial Agricultural Research and Extension Services) ;
  • Rhee, Hye-Kyung (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
  • 투고 : 2009.01.19
  • 심사 : 2010.01.08
  • 발행 : 2010.06.30

초록

조직배양구를 순화시키기 위하여 상토에 정식을 할 때 순화율을 높이고 구 비대에 적합한 재식밀도와 깊이를 구명하고자 시험을 수행되었다. 오리엔탈나리 대표 품종들인 '시베리아'와 '솔본느' 조직배양구의 효과적인 순화를 위한 재식 밀도를 200, 400, 600, 800, 1,000구/$m^2$ 단위로 하였으며, 재식깊이 시험을 위해 상토의 복토를 1, 3, 6, 9, 12cm로 나누어 정식하였다. 오리엔탈나리 '시베리아'의 경우 $m^2$ 당 400구 재식시 생존율 87.3%, 구주 6.4cm, 구중 8.3g, 구주 6cm 이상 비율 61.6%로 가장 적합하였다. '솔본느'의 경우도 400구/$m^2$에서 생존율 88.8%, 구주 5.0cm, 구중 7.1g 으로 가장 효율적인 것으로 생각되었다. 오리엔탈나리 '시베리아'와 '솔본느' 조직배양구의 순화를 위한 적정 재식깊이는 '시베리아'의 경우 지표면에서 3cm 재식구가 생존율 77.8%, 구주 5.9cm, 구중 7.9g, 구주 6cm이상 비율 47.2-51.0%로 가장 양호하였고, '솔본느'의 경우도 3cm 재식구에서 생존율 87.1%, 구주 5.1cm, 구중 6.5g로 가장 양호하였다.

This study was carried out to investigate proper planting density and depth of tissue-cultured oriental lily bulblets for bulb production. Planting densities of bulblets were 200, 400, 600, 800, 1,000 bulblets/ $m^2$, and planting depths were 1, 3, 6, 9, 12 cm. Proper planting density of tissue-cultured 'Siberia' bulblets was 400 bulblets/ $m^2$ with 87.3% of survival rate, 6.4 cm of bulb circumference, and 8.3 g of bulb weight. For 'Sorbonne', it was thought to be 400 bulbs/ $m^2$ with 88.8% of survival rate, 5.0 cm of bulb circumference, and 7.1 g of bulb weight. Proper planting depth of tissue-cultured 'Siberia' bulbs was 3 cm with 77.8% of survival rate, 5.9 cm of bulb circumference, and 7.9 g of bulb weight. For 'Sorbonne' bulbs, it was 3 cm with 87.1% of survival rate, 5.1 cm of bulb circumference, and 6.5 g of bulb weight.

키워드

참고문헌

  1. Han, B.H., B.W. Yae, D.H. Goo, and J.Y. Ko. 1999. Effect of growth regulators and light on the formation and proliferation of bulblets with swollen basal plate from in vitro culture of bulbscales in Lilium oriental hybrid 'Casa Blanca'. J. Kor. Hort. Sci. 40:463-466.
  2. Jeong, M.I., B.N. Chung, W.B. Chae, H.L. Kim, D.H. Goo, and H.S. Choi. 2008. Optimal media and planting depth for acclimation of in vitro propagated oriental lily. Kor. J. Hort. Sci. Technol. 26 (Suppl.):85. (Abstr.)
  3. Kim, E.Y., J.D. Choi, K.I. Park, M.S. Byun, and K.W. Kim. 1999. Enhancement of proliferation rate through multiple shoot induction from culture of microscale section in Lilium. J. Kor. Hort. Sci. 40:459-462.
  4. Kim, K.W., J.S. Kim, E.Y. Kim, J.D. Choi, and K.I. Park. 1998a. Effect of culture conditions on dormant status of in vitro regenerated Lilium oriental hybrid bulblets. J. Kor. Hort. Sci. 39:641-646.
  5. Kim, J.Y., S.T. Choi, M.S. Roh, and T.S Ko. 1996. Production and detection of virus-free lily plants by shoot tip culture and virazole treatment of bulbils. J. Kor. Hort. Sci. 37:64-69.
  6. Kim, J.Y., Y.H. Han, H.S. Soh, S.J. Lee, J.S. Kim, and Y.J. Ra. 1998b. Occurrence of lily viruses and damages of viral diseases in oriental lilies. RDA. J. Crop Protec. 40:58-65.
  7. Kim, S.J., S.Y. Ryu, Y.I. Hahm, and K.Y. Shin. 2000. Aspect of virus occurrend in lily plants according to the periods of successive subcropping. Kor. J. Hort. Sci. Technol. 18:741. (Abstr.)
  8. Lee, K.H., E.M. Lee, and S.S. Ham. 2007. Proper the number of planting bulblets for enlargement of lily cultured in media. 2007. Res. Rept. Chungnam Provincial Agricultural Research and Extension Services.
  9. Ministry for food, agriculture, forestry and fisheries (MIFAFF). 2009. The present condition of floriculture cultivation in 2008. p. 26-27, 162-164.
  10. Ministry of Agriculture, Forestry and Fisheries of Japan. 2009. 2008 Statistics of Agriculture, Forestry and Fisheries. p. 2-19.
  11. Park, K.I., J.D. Choi, I.S. Park, S.J. Eum, and K.W. Kim. 2003. Virus-infected status in imported bulbs of Lilium oriental hybrids. Kor. J. Hort. Sci. Technol. 21:57-61.
  12. Schenk, P.C. and H.K. Rhee. 2007. The globalization of the lily bulb production and breeding in the Netherlands. Flower Res. J. 15:287-297.
  13. Seo, S.Y., H.C. Lim, J. Ryu, and J.S. Na, 1998. Effect of chemoand thermotherapy on LSV inactivation in Lilium oriental hybrid. Kor. J. Hort. Sci. Technol. 16:464. (Abstr.)
  14. Son, B.G., J.S. Kang, Y.J. Lee, and Y.W. Choi. 2002. Effects of physiological properties in culture media on the proliferation and the acclimation of bulblets of lily cultured in vitro. Kor. J. Life Sci. 12:740-744. https://doi.org/10.5352/JLS.2002.12.6.740