Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제36권1호
  • /
  • Pages.16-22
  • /
  • 2010
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  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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마우스에서 히알우론산 나트륨이 항염효과에 미치는 영향에 관한 연구

The effect of hyaluronic acid on anti-inflammatory action in mouse

  • 김상균 (한림대학교 의학과 대학원) ;
  • 이형석 (한림대학교 의학과 대학원) ;
  • 변광섭 (한림대학교 의과대학 강동성심병원 구강악안면외과) ;
  • 이영주 (한림대학교 의과대학 강동성심병원 구강악안면외과) ;
  • 홍순민 (한림대학교 의과대학 강동성심병원 구강악안면외과) ;
  • 최미라 (한림대학교 의과대학 강동성심병원 보철과) ;
  • 박준우 (한림대학교 의과대학 강동성심병원 구강악안면외과)
  • Kim, Sang-Kyun (Hallym University) ;
  • Lee, Hyung-Seok (Hallym University) ;
  • Byeon, Kwang-Seob (Department of Oral and Maxillofacial Surgery, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Lee, Young-Joo (Department of Oral and Maxillofacial Surgery, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Hong, Soon-Min (Department of Oral and Maxillofacial Surgery, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Choi, Mee-Ra (Department of Prosthodontics, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Park, Jun-Woo (Department of Oral and Maxillofacial Surgery, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University)
  • 발행 : 2010.02.26
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초록

Purpose: The purpose of this study was not only to evaluate the relative mRNA expression of interleukin-$1{\beta}$(IL-$1{\beta}$), cyclooxygenase2 (COX-2) and prostaglandin E2 (PGE2) by RT-PCR analysis but to observe pattern of edema by light microscopic and electron microscope after topical apply of hyaluronic acid in inflammation-guided mouse. Material and methods: Mice of this study were devided into 4 groups: Control group (no inflammation guided), Positive control (inflammation guided + vaselin apply), Protopic group (inflammation guided + protopic apply), Hyaluronic group (inflammation guided + hyaluronic acid apply). Results: Hyaluronic group showed less expressions of IL-$1{\beta}$, COX-2, PGE2 than those of positive control & protopic group. Hyaluronic group revealed a decreased inflammation than positive control & protopic group in Light Microscope. Hyaluronic group appeared decreased edema of ear compare to positive control & protopic group in Elecron Microscope. Conclusion: It was considered that hyaluronic acid has an antiinflammatory effect for intercepting the gene expression of cytokines related to inflammation.

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참고문헌

  1. Altman RD, Moskowitz R. A randomized clinical trial of intraarticular sodium hyaluronate in patients with osteoarthritis of the knee: a summary. Am J Orthop (Belle Mead NJ) 1999;28:3-4.
  2. Caporossi A, Baiocchi S, Sforzi C, Frezzotti R. Healon GV versus Healon in demanding cataract surgery. J Cataract Refract Surg 1995;21:710-3. https://doi.org/10.1016/S0886-3350(13)80572-X
  3. Matsuno H, Yudoh K, Kondo M, Goto M, Kimura T. Bioche-mical effect of intra-articular injections of high molecular weight hyaluronate in rheumatoid arthritis patients. Inflamm Res 1999;48:154-9. https://doi.org/10.1007/s000110050439
  4. Liguori V, Guillemin C, Pesce GF, Mirimanoff RO, Bernier J. Double-blind, randomized clinical study comparing hyaluronic acid cream to placebo in patients treated with radiotherapy. Radiother Oncol 1997;42:155-61. https://doi.org/10.1016/S0167-8140(96)01882-8
  5. Huskisson EC, Donnelly S. Hyaluronic acid in the treatment of osteoarthritis of the knee. Rheumatology (Oxford) 1999;38:602-7. https://doi.org/10.1093/rheumatology/38.7.602
  6. Adams ME, Atkinson MH, Lussier Aj, Schulz JI, Siminovitch KA, Wade JP, et al. The role of viscosupplementation with hylan G-F 20 (Synvisc) in the treatment of osteoarthritis of the knee: a Canadian multicenter trial comparing hylan G-F 20 alone, hylan G-F 20 with non-steroidal anti-inflammatory drugs (NSAIDs) and NSAIDs alone. Osteoarthritis Cartilage 1995;3:213-25. https://doi.org/10.1016/S1063-4584(05)80013-5
  7. Kim HJ, Shin CH, Hong J, Choi JH. The effects of hyaluronic acid gel on the healing of oral mucosa. J Korean Assoc Oral Maxillofac Surg 2007;33:359-66.
  8. Rosa DS, Aranha AC, Eduardo Cde P, Aoki A. Esthetictreatment of gingival melanin hyperpigmentation with Er:YAG laser: shortterm clinical observations and patient follow-up. J. Periodontol. 2007 Oct:78(10):2018-25. https://doi.org/10.1902/jop.2007.070041
  9. Pradeep AR, Kumar MS, Ramachandraprasad MV, Shikha C. Gingival crevicular fluid levels of neopterin in healthy subjects and in patients with different periodontal diseases. J Periodontol 2007;78:1962-7. https://doi.org/10.1902/jop.2007.070096
  10. Villa AA. Clinical, biochemical and histopatological correlacion in diabetic patiens with periodontal disease. Rev Fac Cien Med Univ Nac Cordoba 2006;63(2S):50-5.
  11. Vangelisti R, Pagnacco O, Erra C. Hyaluronic acid in the topical treatment of gingival inflammations: preliminary clinical trial. Attualita Terapeutica Int 1997;15:2-3.
  12. Pagnacco A, Vangelisti R, Erra C, Poma A. Double-blind clinical trial vs. placebo of a new sodium-hyaluronate-based gingival gel. Attualita Terapeutica Int 1997;15:1-7.
  13. Rabasseda X. The role of hyaluronic acid in the management of periodontal disease. Drugs Today (Barc) 2000;36(S):1-20.
  14. Jentsch H, Pomowski R, Kundt G, Gocke R. Treatment of gingivitis with hyaluronan. J Clin Periodontol 2003;30:159-64. https://doi.org/10.1034/j.1600-051X.2003.300203.x
  15. Pistorius A, Martin M, Willershausen B, Rockmann P. The clinical application of hyaluronic acid in gingivitis therapy. Quintessence Int 2005;36:531-8.
  16. Toole BP, Jackson G, Gross J. Hyaluronate in morphogenesis: inhibition of chondrogenesis in vitro. Proc Natl Acad Sci U S A 1972;69:1384-6. https://doi.org/10.1073/pnas.69.6.1384
  17. Meyer K, Palmer JW. The polysaccharide of the vitreous humor. J Biol Chem 1934;107:629-34.
  18. Heldin P, Laurent TC, Heldin CH. Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts. Biochem J 1989;258:919-22. https://doi.org/10.1042/bj2580919
  19. Ghosh K, Ren XD, Shu XZ, Prestwich GD, Clark RA. Fibronectin functional domains coupled to hyaluronan stimulate adult human dermal fibroblast responses critical for wound healing. Tissue Eng 2006;12:601-13. https://doi.org/10.1089/ten.2006.12.601
  20. Voinchet V, Vasseur P, Kern J. Efficacy and safety of hyaluronic acid in the management of acute wounds. Am J Clin Dermatol 2006;7:353-7. https://doi.org/10.2165/00128071-200607060-00003
  21. Nolan A, Baillie C, Badminton J, Rudralingham M, Seymour RA. The efficacy of topical hyaluronic acid in the management of recurrent aphthous ulceration. J Oral Pathol Med 2006;35:461-5. https://doi.org/10.1111/j.1600-0714.2006.00433.x
  22. LeBeouf RD, Gregg R, Weigel PH, Fuller GM. Effects of hyaluronic acid and other glycosaminoglycans on fibrin polymer formation. Biochemistry 1987;26:6052-7. https://doi.org/10.1021/bi00393a016
  23. Wheeler JC, Woods JA, Cox MJ, Cantrell RW, Watkins FH, Edlich RF. Evolution of hydrogel polymers as contact lenses, surface coatings, dressings, and drug delivery systems. J Long Term Eff Med Implants 1996;6:207-17.
  24. Jaffe BM. The role of prostaglandins, thromboxane, and leukotrienes in surgery. In: In: Sabiston DC Jr, ed. Textbook of surgery: the biological basis of modern surgical practice. 14th ed. Philadelphia: WB Saunders; 1991:465-9.
  25. Davidson EM, Rae SA, Smith MJ. Leukotriene B4, a mediator of inflammation present in synovial fluid in rheumatoid arthritis. Ann Rheum Dis 1983;42:677-9. https://doi.org/10.1136/ard.42.6.677
  26. Smith WL, Meade EA, Dewitt DL. Interaction of PGH synthase isozymes-1 and -2 with nonsteroidal anti-inflammatory drugs. Adv Exp Med Biol 1997;400A:189-96.