Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제36권1호
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  • Pages.1-6
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  • 2010
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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구강편평상피암종에서 stromal cell-derived factor-1의 발현

Stromal cell-derived factor-1 (SDF-1) expression in the oral squamous cell carcinoma

  • 김경욱 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 한세진 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 노규섭 (단국대학교 치과대학 구강악안면외과학교실)
  • Kim, Kyung-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Han, Se-Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Roh, Kyu-Seob (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
  • 발행 : 2010.02.26
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초록

Purpose: Chemokines are structurally related, small polypeptide signaling molecules that bind to and activate a family of transmembrane G protein-coupled receptors, the chemokine receptors. Recently, interaction between the chemokine receptor CXCR4 and its ligand, stromal cell-derived factor 1 (SDF-1 or CXCL12), has been found to play an important role in tumorigenicity, proliferation, metastasis and angiogenesis in many cancers such as lung cancer, breast cancer, melanoma, glioblastoma, pancreatic cancer and cholangiocarcinoma. Hence, the goal of this study is to identify the correlation of clinicopathological factors and the up-regulation of SDF-1 expression in oral squamous cell carcinoma. Material and methods: We studied the immunohistochemical staining of SDF-1, quantitative RT-PCR (qRT-PCR) of SDF-1 gene in 20 specimens of 20 patients with oral squamous cell carcinoma. Results: 1. In the immunohistochemical study of poor differentiated and invasive oral squamous cell carcinoma, the high level staining of SDF-1 was observed. And the correlation between immunohistochemical SDF-1 expression and tumor nodes metastases (TNM) classification of specimens was significant.($x^2$ test, P < 0.05) 2. In the SDF-1 gene qRT-PCR analysis, SDF-1 expression was more in tumor tissue than in carcinoma in situ tissue. Paired-samples analysis determined the difference of SDF-1 mRNA expression level between the cancer tissue and the carcinoma in situ tissue.(Student's t-test, P < 0.05) Conclusion: These findings suggest that up-regulation of the SDF-1 may play a role in progression and invasion of oral squamous cell carcinoma.

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참고문헌

  1. Sankaranarayanan R, Masuyer E, Swaminathan R, Ferlay J, Whelan S. .Head and neck cancer: a global perspective on epidemiology and prognosis. Anticancer Res 1998;18:4779-86.
  2. Parkin DM, Bray F, Ferlay J, Pisani P. Estimating the world cancer burden: Globocan 2000. Int J Cancer 2001;94:153-6. https://doi.org/10.1002/ijc.1440
  3. Carvalho AL, Ikeda MK, Magrin J, Kowalski LP. Trends of oral and oropharyngeal cancer survival over five decades in 3267 patients treated in a single institution. Oral Oncol 2004;40:71-6. https://doi.org/10.1016/S1368-8375(03)00138-6
  4. Pisani P. Parkin DM, Bray F, Ferlay J. Estimates of the worldwide mortality from 25 cancers in 1990. Int J Cancer 1999;83:18-29.
  5. Parkin DM, Pisani P, Ferlay J. Estimates of the worldwide incidence of 25 major cancers in 1990. Int J Cancer 1999;80:827-41. https://doi.org/10.1002/(SICI)1097-0215(19990315)80:6<827::AID-IJC6>3.0.CO;2-P
  6. Carvalho AL, Magrin J, Kowalski LP. Sites of recurrence in oral and oropharyngeal cancers according to the treatment approach. Oral Dis 2003;9:112-8. https://doi.org/10.1034/j.1601-0825.2003.01750.x
  7. Hemmer J, Kreidler J. Flow cytometric DNA ploidy analysis of squamous cell carcinoma of the oral cavity. Comparison with clinical staging and histologic grading. Cancer 1990;66:317-20. https://doi.org/10.1002/1097-0142(19900715)66:2<317::AID-CNCR2820660220>3.0.CO;2-X
  8. Fernandez EJ, Lolis E. Structure, function, and inhibition of chemokines. Annu Rev Pharmacol Toxicol 2002;42:469-99. https://doi.org/10.1146/annurev.pharmtox.42.091901.115838
  9. Laing KJ, Secombes CJ. Chemokines. Dev Comp Immunol 2004;28:443-60. https://doi.org/10.1016/j.dci.2003.09.006
  10. Murdoch C, Finn A. Chemokine receptors and the role in inflammation and infectious diseases. Blood 2000;95:3032-43.
  11. Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Murakami K, Miyake Y, et al. CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease. Clin Exp Immunol 2005;141:467-74. https://doi.org/10.1111/j.1365-2249.2005.02852.x
  12. Rossi D. Zlotnik A. The biology of chemokines and their receptors. Annu Rev Immunol 2000;18:217-42. https://doi.org/10.1146/annurev.immunol.18.1.217
  13. Muller A, Homey B, Soto H, Ge N, Catron D, Buchanan M, et al. Involvement of chemokine receptors in breast cancer metastasis. Nature 2001;410:50-6. https://doi.org/10.1038/35065016
  14. Taichman RS, Cooper C, Keller ET, Pienta KJ, Taichman NS, McCauley LK. Use of the stromal cell-derived factor-1/CXCR4 pathway in prostate cancer metastasis to bone. Cancer Res 2002;62:1832-7.
  15. Scotton CJ, Wilson JL, Milliken D, Stamp G, Balkwill FR. Epithelial cancer cell migration: a role for chemokine receptors? Cancer Res 2001;61:4961-5.
  16. Uchida D, Begum NM, Almofti A, Nakashiro K, Kawamata H, Tateishi Y, et al. Possible role for stromal-cell-derived factor-1/CXCR4 signaling on lymph node metastasis of oral squamous cell carcinoma. Exp Cell Res 2003;290:289-302. https://doi.org/10.1016/S0014-4827(03)00344-6
  17. Bertolini F, Dell'Agnola C, Mancuso P, Rabascio C, Burlini A, Monestiroli S, et al. CXCR4 neutralization, a novel therapeutic approach for non-Hodgkin's lymphoma. Cancer Res 2002;62:3106-12.
  18. Murakami T, Maki W, Cardones AR, Fang H, Tun Kyi A, Nestle FO. Expression of CXC chemokine receptor-4 enhances the pulmonary metastatic potential of murine B16 melanoma cells. Cancer Res 2002;62: 7328-34.
  19. Ghadjar P, Coupland SE, Na IK, Noutsias M, Letsch A, Stroux A,et al. Chemokine receptor CCR6 expression level and liver metastases in colorectal cancer. J Clin Oncol 2006;24:1910-6. https://doi.org/10.1200/JCO.2005.04.1822
  20. Kang H, Watkins G, Parr C, Douglas-Jones A, Mansel RE, Jiang WG. Stromal cell derived factor-1: its influence on invasiveness and migration of breast cancer cells in vitro, and its association with prognosis and survival in human breast cancer. Breast Cancer Research 2005;7:R402-10. https://doi.org/10.1186/bcr1022
  21. Daly AJ, McIlreavey L, Irwin CR. Regulation of HGF and SDF-1 expression by oral fibroblasts-implications for invasion of oral cancer. Oral Oncol 2008;44:646-51. https://doi.org/10.1016/j.oraloncology.2007.08.012
  22. Kim H, Muller WJ. The role of the epidermal growth factor receptor family in mammary tumorigenesis and metastasis. Exp Cell Res 1999;253:78-87. https://doi.org/10.1006/excr.1999.4706
  23. Youngs SJ, Ali SA, Taub DD, Rees RC. Chemokines induce migrational responses in human breast carcinoma cell lines. Int J Cancer 1997;71:257-66. https://doi.org/10.1002/(SICI)1097-0215(19970410)71:2<257::AID-IJC22>3.0.CO;2-D
  24. Hill MW, Mackenzie IC. The influence of differing connective tissue substrates on the maintenance of adult stratified squamous epithelia. Cell Tissue Res 1984;237:473-8.
  25. Mirshahi F. Pourtau J, Li H, Muraine M, Trochon V, Legrand E, et al. SDF-1 activity on microvascular endothelial cells: consequences on angiogenesis in in vitro and in vivo models. Thromb Res 2000;99:587-94. https://doi.org/10.1016/S0049-3848(00)00292-9
  26. Molino M, Woolkalis MJ, Prevost N, Pratico′D, Barnathan ES, Taraboletti G, et al. CXCR4 on human endothelial cells can serve as both a mediator of biological responses and as a receptor for HIV-2. Biochim Biophys Acta 2000;1500:227-40. https://doi.org/10.1016/S0925-4439(99)00110-6
  27. Yang SX, Chen JH, Jiang XF, Wang QL, Chen ZQ, Zhao W, et al. Activation of chemokine receptor CXCR4 in malignant glioma cells promotes the production of vascular endothelial growth factor. Biochem Biophys Res Commun 2005;335:523-8. https://doi.org/10.1016/j.bbrc.2005.07.113
  28. Datta SR, Brunet A, Greenberg ME. Cellular survival: a play in three Akts. Genes Dev 1999;13:2905-27. https://doi.org/10.1101/gad.13.22.2905
  29. Evan GI, Vousden KH. Proliferation, cell cycle and apoptosis in cancer. Nature 2001;411:342-8. https://doi.org/10.1038/35077213
  30. Dewan MZ, Ahmed S, Iwasaki Y, Ohba K, Toi M, Yamamoto N. Stromal cell-derived factor-1 and CXCR4 receptor interaction in tumor growth and metastasis of breast cancer. Biomed Pharmacother 2006;60:273-6. https://doi.org/10.1016/j.biopha.2006.06.004
  31. Strieter RM, Burdick MD, Mestas J, Gomperts B, Keane MP, Belperio JA. Cancer CXC chemokine networks and tumour angiogenesis. Eur J Cancer 2006;42:768-78. https://doi.org/10.1016/j.ejca.2006.01.006