Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지
DOI QR코드

DOI QR Code

Comparative genetic diversity of wild and released populations of Pacific abalone Haliotis discus discus in Jeju, Korea, based on cross-species microsatellite markers including two novel loci

  • An, Hye-Suck (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Hong, Seong-Wan (Jeju Province Fisheries Research Institute) ;
  • Kim, En-Mi (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Noh, Jae-Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Hyun-Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Park, Chul-Ji (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Myeong, Jeong-In (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
  • Received : 2010.05.27
  • Accepted : 2010.08.01
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Pacific abalone Haliotis discus discus is an important fisheries resource in Jeju, Korea. For basic information about its current genetic status in relation to stock enhancement, the level and distribution of genetic variation between wild and released stocks of Pacific abalone in Jeju were examined at nine cross-species microsatellite markers including the use of two novel primers. High levels of polymorphism were observed between the two populations. A total of 146 different alleles were found at all loci, with some alleles being unique. The allelic variability ranged from five to 27 in the wild population and from four to 16 in the released sample. The average observed and expected heterozygosities were estimated to be 0.74 and 0.84 in the wild sample and 0.70 and 0.78 in the released sample, respectively. Although a considerable loss of rare alleles was observed in the released sample, no statistically significant reductions were found in heterozygosity or allelic diversity in the released sample compared to the wild population. Low but significant genetic differentiation was found between the wild and released populations. These results suggest that the intensive breeding practices for stock enhancement may have resulted in a further decrease in genetic diversity, and that the cross-species microsatellite markers used in this study represent a potentially efficient means for further genetic studies, providing beneficial information for the protection and management of H. discus discus.

Keywords

References

  1. Alarcon JA, Magoulas A, Georgakopoulos T, Zouros E, Alvarez MC. 2004. Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata). Aquaculture. 230:65-80. https://doi.org/10.1016/S0044-8486(03)00434-4
  2. Allendorf FW, Phelps SR. 1980. Loss of genetic variation in a hatchery stock of cutthroat trout. Trans. Am Fish Soc. 109:537-543. https://doi.org/10.1577/1548-8659(1980)109<537:LOGVIA>2.0.CO;2
  3. An HS, Han SJ. 2005. Isolation and characterization of microsatellite DNA markers in the Pacific abalone, Haliotis discus hannai. Mol Ecol Notes. 6:11-13.
  4. An HS, Jee YJ, Min K-S, Kim B-L, Han SJ. 2005. Phylogenetic analysis of six species of Pacific abalone (Haliotidae) based on DNA sequences of 16s rRNA and cytochrome c oxidase subunit I mitochondrial genes. Mar Biotec. 7:373-380. https://doi.org/10.1007/s10126-004-4405-2
  5. An HS, Kim MJ, Hong SW. 2008. Genetic diversity of rock bream Oplegnathus fasciatus in southern Korea. Genes Genomics. 30:451-459.
  6. An HS, Lee J-H, Noh JK, Kim HC, Park CJ, Min BH, Myeong J-I. 2010. New polymorphic microsatellite markers in pacific abalone Haliotis discus hannai and their application to genetic characterization of wild and aquaculture populations. Genes Genomics. 32:413-418. https://doi.org/10.1007/s13258-010-0037-2
  7. Banks MA, Blouin MS, Baldwin BA, Rashbrook VK, Fitzgerald HA, Blankenship SM, Hedgecock D. 1999. Isolation and inheritance of novel microsatellites in Chinook salmon (Oncorhynchus tschawytscha). J Hered. 90:281-288. https://doi.org/10.1093/jhered/90.2.281
  8. Chen L, Li Q, Yang J. 2008. Microsatellite genetic variation in wild and hatchery populations of the sea cucumber (Apostichopus japonicas Seleka) from northern China. Aqua Res. 39:1541-1549. https://doi.org/10.1111/j.1365-2109.2008.02027.x
  9. Coughlan JP, Imsland AK, Galvin PT, Fitzgerald RD, Naevdal G, Cross. 1998. Microsatellite DNA variation in wild populations and farmed strains of turbot from Ireland and Norway: a preliminary study. J Fish Biol. 52:916-922. https://doi.org/10.1111/j.1095-8649.1998.tb00592.x
  10. Elliott NG, Reilly A. 2003. Likelihood of a bottleneck even in the history of the Australian population of Atlantic salmon (Salmo salar L.). Aquaculture. 215:31-44. https://doi.org/10.1016/S0044-8486(02)00055-8
  11. Estoup A, Tailliez C, Cornuet J, Solignac M. 1995. Size homoplasy and mutational processes of interrupted microsatellites in two bee species, Apis mellifera and Bombus terrestris (Apidae). Mol Biol Evol. 12:1074-1084.
  12. Excoffier L, Laval G, Schneider S. 2005. ARLEQUIN version 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online. 1:47-50.
  13. FAO. 1993. Report of the expert consultation on utilization and conservation of aquatic genetic resources. FAO Fish Rep. 491:1-58.
  14. Goudet J. 2002. FSTAT: a computer program to calculate Fstatistics. J Hered. 86:485-486.
  15. Gum B, Gross R, Geist J. 2009. Conservation genetics and management implications for European grayling, Thymallus thymallus: synthesis of phylogeography and population genetics. Fish Manag Ecol. 16:37-51. https://doi.org/10.1111/j.1365-2400.2008.00641.x
  16. Hansen MM. 2002. Estimating the long-term effects of stocking domesticated trout into wild brown trout (Salmo trutta) populations: an approach using microsatellite DNA analysis of historical and contemporary samples. Mol Ecol. 11:1003-1015. https://doi.org/10.1046/j.1365-294X.2002.01495.x
  17. Hara M, Sekino M. 2003. Efficient detection of parentage in a cultured Japanese flounder Paralichthys olivaceus using microsatellite DNA marker. Aquaculture. 217:107-114. https://doi.org/10.1016/S0044-8486(02)00069-8
  18. Hara M, Sekino M. 2005. Genetic difference between Ezoawabi Haliotis discus hannai and Kuro-awabi Haliotis discus discus populations: microsatellite-based population analysis in Japanese abalone. Fish Sci. 71:754-766. https://doi.org/10.1111/j.1444-2906.2005.01025.x
  19. Hindar K, Ryman N, Utter FM. 1991. Genetic effects of cultured fish on natural fish populations. Can J Fish Aqua Sci. 48:945-957. https://doi.org/10.1139/f91-111
  20. Ino T. 1952. Biological study on the propagation of Japanese abalone (genus Haliotis). Bull Tokai Reg Fish Res Lab.5:1-102.
  21. Jeong DS, Umino T, Kuroda K, Hayashi M, Nakagawa H, Kang JC, Morishima K, Arai K. 2003. Genetic divergence and population structure of black sea bream Acanthopagrus schlegeli inferred from microsatellite analysis. Fish Sci. 69:896-902. https://doi.org/10.1046/j.1444-2906.2003.00705.x
  22. Li Q, Park C, Kijima A. 2002. Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai. J Shell Res. 212:811-815.
  23. Li Q, Park C, Kobayashi A, Kijima A. 2003. Inheritance of microsatellite DNA markers in the Pacific abalone Haliotis discus hannai. Mar Biotec.5:331-338. https://doi.org/10.1007/s10126-002-0116-8
  24. Li Q, Park C, Endo T, Kijima A. 2004. Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai). Aquaculture. 235:207-222. https://doi.org/10.1016/j.aquaculture.2003.12.018
  25. Li Q, Shu J, Yu R, Tian C. 2007. Genetic variability of cultured populations of the Pacific abalone (Haliotis discus hannai Ino) in China based on microsatellites. Aquaculture Res. 38:981-990. https://doi.org/10.1111/j.1365-2109.2007.01764.x
  26. Marchant S, Haye PA, Marin SA, Winkler FM. 2008. Genetic variability revealed with microsatellite markers in an introduced population of the abalone Haliotis discus hannai Ino. Aquaculture Res. 40:298-304.
  27. Michalakis Y, Excoffier L. 1996. A genetic estimation of population subdivision using distances between alleles with special reference for microsatellite loci. Genetics. 142:1061-1064.
  28. Porta J, Porta JM, Martinez-Rodriguez G, Alvarez MC. 2006. Genetic structure and genetic relatedness of a hatchery stock of Senegal sole (Solea senegalensis) inferred by microsatellites. Aquaculture. 251:46-55. https://doi.org/10.1016/j.aquaculture.2005.05.019
  29. Primmer CR, Aho T, Piironen J, Estoup A, Cornuet JM, Ranta E. 1999. Microsatellite analysis of hatchery stocks and natural populations of Arctic charr, Salvelinus alpinus, from the Nordic region: implications for conservation. Hereditas. 130:277-289.
  30. Rice WR. 1989. Analyzing tables of statistical tests. Evolution. 43:223-225. https://doi.org/10.2307/2409177
  31. Rousset F, Raymond M. 1995. Testing heterozygote excess and deficiency. Genetics. 140:1413-1419.
  32. Sekino M, Hara M. 2001. Application of microsatellite DNA markers to population genetics studies of Japanese flounder Paralichthys olivaceus. Mar Biotec. 3:572-589. https://doi.org/10.1007/s10126-001-0064-8
  33. Sekino M, Hara M, Taniguchi N. 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthys olivaceus. Aquaculture. 213:101-122. https://doi.org/10.1016/S0044-8486(01)00885-7
  34. Sekino M, Saido T, Fujita T, Kobayashi T, Takami H. 2005. Microsatellite DNA markers of Ezo abalone (Haliotis discus hannai): a preliminary assessment of natural populations sampled from heavily stocked areas. Aquaculture. 243:33-47. https://doi.org/10.1016/j.aquaculture.2004.10.013
  35. Skaala O, Hoyheim B, Glover K, Dahle G. 2004. Microsatellite analysis in domesticated and wild Atlantic salmon (Salmo salar L.): allelic diversity and identification of individuals. Aquaculture. 240:131-143. https://doi.org/10.1016/j.aquaculture.2004.07.009
  36. Slatkin M. 1995. A measure of population subdivision based on microsatellite allele frequencies. Genetics. 139:457-462.
  37. Slatkin M, Excoffier L. 1996. Testing for linkage disequilibrium in genotypic data using the EM algorithm. Heredity. 76:377-383. https://doi.org/10.1038/hdy.1996.55
  38. Stahl G. 1987. Genetic population structure of Atlantic salmon. In: Ryman N, Utter F, editors. Population genetics and fishery management. Seattle: University Washington Press. p. 121-140.
  39. Tessier N, Bernatchez L, Wright JM. 1997. Population structure and impact supportive breeding inferred from mitochondrial and microsatellite DNA analyses in landlocked Atlantic salmon Salmo salar L. Mol Ecol. 6:735-750. https://doi.org/10.1046/j.1365-294X.1997.00244.x
  40. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes. 4:135.
  41. Vuorinen J. 1984. Reduction of genetic variability in a hatchery stock of brown trout, Salmo trutta. J Fish Biol. 24:339-348. https://doi.org/10.1111/j.1095-8649.1984.tb04805.x
  42. Was A, Wenne R. 2002. Genetic differentiation in hatchery and wild sea trout (Salmo trutta) in the southern Baltic at microsatellite loci. Aquaculture. 204:493-506. https://doi.org/10.1016/S0044-8486(01)00835-3
  43. Weir BS, Cockerham CC. 1984. Estimating F-statistics for the analysis of population structure. Evolution. 38:1358-1370. https://doi.org/10.2307/2408641

Cited by

  1. Genetic Characterization of Five Hatchery Populations of the Pacific Abalone ( Haliotis discus hannai ) Using Microsatellite Markers vol.12, pp.8, 2010, https://doi.org/10.3390/ijms12084836
  2. Geographic homogeneity and high gene flow of the pear psylla, $Cacopsylla$ $pyricola$ (Hemiptera: Psyllidae), detected by mitochondrial COI gene and nuclear ribosomal internal transcribed spacer 2 vol.16, pp.2, 2010, https://doi.org/10.1080/19768354.2011.607511
  3. Development and characterization of nine microsatellite loci from the Korean hare (Lepus coreanus) and genetic diversity in South Korea vol.16, pp.3, 2010, https://doi.org/10.1080/19768354.2011.640351
  4. Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis vol.11, pp.4, 2010, https://doi.org/10.4238/2012.november.12.8