Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins

  • Heo, Jee-In (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Oh, Soo-Jin (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kho, Yoon-Jung (Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kim, Jeong-Hyeon (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kang, Hong-Joon (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Park, Seong-Hoon (Molecular Radiation Oncology, Radiation Oncology Branch, Center for Cancer Research, NCI, NIH) ;
  • Kim, Hyun-Seok (Molecular Radiation Oncology, Radiation Oncology Branch, Center for Cancer Research, NCI, NIH) ;
  • Shin, Jong-Yeon (Genomic Medicine Institute, Medical Research Center, Seoul National University) ;
  • Lee, Sung-Young (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kim, Min-Ju (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Min, Bon-Hong (Department of Pharmacology, College of Medicine, Korea University) ;
  • Kim, Sung-Chan (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kim, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Lee, Jae-Yong (Department of Biochemistry, College of Medicine, Hallym University)
  • Received : 2010.07.20
  • Accepted : 2010.09.24
  • Published : 2010.12.31
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Abstract

PC12 cells were differentiated into the cells of chromaffin phenotype by butyrate treatment. Cells were aggregated and formed tight cell adhesion. To investigate the molecular change in this differentiation, we examined expression levels of cell adhesion proteins and extracellular proteins during butyrate induced-differentiation of PC12 cells. Integrin ${\beta}1$, integrin ${\alpha}7$, E cadherin, VCAM, collagen-I, fibronectin, desmoglein and connexin were increased during differentiation. The levels of clusterin and secreted clusterin were also increased. These increased levels of cell adhesion proteins and extracellular proteins appear to induce cell aggregation and tight cell adhesion. The levels of p21, p27 and p16 were increased probably because of differentiation-related growth arrest during differentiation. Prolonged incubation of butyrate up to 1 day was required for differentiation. Signal transduction pathways for this differentiatiom could not be identified since various inhibitors had no effect. The results showed that butyrateinduced differentiation of PC12 cells to chromaffin cells involves tight cell adhesion and induction of extracellular proteins and cell adhesion proteins.

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References

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