Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Effects of Nitric Oxide on the Neuronal Activity of Rat Cerebellar Purkinje Neurons

  • Jang, Su-Joong (Department of Physiology, Chonnam National University Medical School) ;
  • Jeong, Han-Soong (Department of Physiology, Chonnam National University Medical School) ;
  • Park, Jong-Seong (Department of Physiology, Chonnam National University Medical School)
  • ;
  • ;
  • 박종성 (전남대학교 의과대학 생리학교실)
  • Received : 2010.10.21
  • Accepted : 2010.12.29
  • Published : 2010.12.31
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Abstract

This study was designed to investigate the effects of nitric oxide on the neuronal activity of rat cerebellar Purkinje cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated Purkinje cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium current were recorded by standard patch-clamp techniques under current and voltage-clamp modes respectively. 15 Purkinje cells revealed excitatory responses to $20\;{\mu}M$ of sodium nitroprusside (SNP) and 4 neurons (20%) did not respond to SNP. Whole potassium currents of Purkinje cells were decreased by SNP (n=10). Whole potassium currents of Purkinje cells were also decreased by L-arginine, substrate of nitric oxide (n=10). These experimental results suggest that nitric oxide increases the neuronal activity of Purkinje cells by altering the resting membrane potential and after hyperpolarization.

Keywords

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