Parasites, Hosts and Diseases

  • 제48권4호
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  • Pages.319-324
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  • 2010
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Cloning of Plasmodium vivax Calcium-Dependent Protein Kinase 4

  • Choi, Kyung-Mi (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Kim, Jung-Yeon (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Moon, Sung-Ung (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Lee, Hyeong-Woo (Department of Pathology, University of Florida) ;
  • Sattabongkot, Jetsumon (Department of Entomology, Armed Forces Research Institute of Medical Science) ;
  • Na, Byoung-Kuk (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kim, Dae-Won (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Suh, Eun-Jung (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Kim, Yeon-Joo (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Cho, Shin-Hyeong (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention) ;
  • Lee, Ho-Sa (Institute of Global Environment and Department of Biology, Kyung Hee University) ;
  • Rhie, Ho-Gun (Institute of Global Environment and Department of Biology, Kyung Hee University) ;
  • Kim, Tong-Soo (Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention)
  • 투고 : 2010.07.04
  • 심사 : 2010.11.01
  • 발행 : 2010.12.31
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초록

A family of calcium-dependent protein kinases (CDPKs) is a unique enzyme which plays crucial roles in intracellular calcium signaling in plants, algae, and protozoa. CDPKs of malaria parasites are known to be key regulators for stage-specific cellular responses to calcium, a widespread secondary messenger that controls the progression of the parasite. In our study, we identified a gene encoding Plasmodium vivax CDPK4 (PvCDPK4) and characterized its molecular property and cellular localization. PvCDPK4 was a typical CDPK which had well-conserved N-terminal kinase domain and C-terminal calmodulin-like structure with 4-EF hand motifs for calcium-binding. The recombinant protein of EF hand domain of PvCDPK4 was expressed in Echerichia coli and a 34 kDa product was obtained. Immunofluorescence assay by confocal laser microscopy revealed that the protein was expressed at the mature schizont of P. vivax. The expression of PvCDPK4-EF in schizont suggests that it may participate in the proliferation or egress process in the life cycle of this parasite.

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참고문헌

  1. Arevalo-Herrera M, Herrera S. Plasmodium vivax malaria vaccine development. Mol Immunol 2001; 38: 443-455. https://doi.org/10.1016/S0161-5890(01)00080-3
  2. Breman JG. The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden. Am J Trop Med Hyg 2001; 64: 1-11.
  3. Snow RW, Trape JF, Marsh K. The past, present and future of childhood malaria mortality in Africa. Trends Parasitol 2001; 17: 593-597. https://doi.org/10.1016/S1471-4922(01)02031-1
  4. Ishino T, Orito Y, Chinzei Y, Yuda M. A calcium-dependent protein kinases regulates Plasmodium ookinete access to the midgut epithelial cell. Mol Microbiol 2006; 59: 1175-1184. https://doi.org/10.1111/j.1365-2958.2005.05014.x
  5. Billker O, Lourido S, Sibley LD. Calcium dependent signaling and kinases in apicomplexan parasites. Cell Host Microbe 2009; 5: 612-622. https://doi.org/10.1016/j.chom.2009.05.017
  6. Billker O, Dechamps S, Tewari R Wenig G, Franke-Fayard B, Brinkmann V. Calcium and a calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite. Cell 2004; 117: 503-514. https://doi.org/10.1016/S0092-8674(04)00449-0
  7. Farber PM, Graeser R Franklin RM, Kappes B. Molecular cloning and characterization of a second calcium-dependent protein kinase of Plasmodium falciparum. Mol Biochem Parasitol 1997; 87: 211- 216. https://doi.org/10.1016/S0166-6851(97)00052-2
  8. Harper JF, Harmon A. Plant, symbiosis and parasites: a calcium signaling connection. Nat Rev Mol Cell Bioi 2005; 6: 555-566. https://doi.org/10.1038/nrm1679
  9. Aurrecoechea C, Brestelli J, Brunk BP, Dommer J, Fischer S, Gajria B, Gao X, Gingle A, Grant G, Harb OS, Heiges M, Innamorato F, Iodice J, Kissinger JC, Kraemer E, Li W, Miller JA, Nayak V, Pennington C, Pinney DF, Roos DS, Ross C, Stoeckert CJ Jr, Treatman C, Wang H. PlasmoDB: a functional genomic database for malaria parasites. Nucleic Acids Res 2009; 37: 0539-543.
  10. Mott R. EST_GENOME: A program to align spliced DNA sequences to unspliced genomic DNA. Comput Appl Biosci 1997; 13: 477-478.
  11. Kiefer F, Arnold K, Kunzli M, Bordoli L, Schwede T. The SWISSMODEL repository and associated resources. Nucleic Acids Res 2009; 37: 0387-392. https://doi.org/10.1093/nar/gkn750
  12. Angel DI, Mongui A. Ardila J, Vanegas M, Patarroyo MA. The Plasmodium vivax Pv41 surface protein: identification and characterization. Biochem Biophys Res Commun 2008; 377: 1113-1117. https://doi.org/10.1016/j.bbrc.2008.10.129
  13. Green JL, Ress-Channer R, Howell SA, Martin SR, Knuepfer E, Taylor HM, Grainger M, Holder M. The motor complex of Plasmodium falciparum: phosphoryation by a calcium-dependent protein kinase. J Biol Chem 2008; 283: 30980-30989. https://doi.org/10.1074/jbc.M803129200
  14. Bozelech Z, Mok S, Hu G, lmwong M, Jaidee A. Russell B, Ginsburg H, Nosten F, Day NP, White NJ, Carlton JM, Preiser PR. The transcriptome of Plasmodium vivax reveals divergence and diversity of transcriptional regulation in malaria parasites. Proc Natl Acad Sci USA 2008; 105: 16290-16295. https://doi.org/10.1073/pnas.0807404105
  15. Moudy R, ManningTJ, Beckers CJ. The loss of cytoplasmic potassium upon host cell breakdown triggers egress of Toxoplasma gondii. J Biol Chem 2001; 276: 41492-41501. https://doi.org/10.1074/jbc.M106154200
  16. Sugi T, Kato K, Kobayashi K, Pandey K, Takemae H, Kurokawa H, Tohya Y, Akashi H. Molecular analyses of Toxoplasma gondii calmodulin-like domain protein kinase isoform 3. Parasitol lnt 2009; 58: 416-423. https://doi.org/10.1016/j.parint.2009.08.005
  17. Ranjan R, Ahmed A, Gourinath S, Sharma P. Dissection of mechanisms involved in the regulation of Plasmodium falciparum calcium-dependent protein kinase 4. J Biol Chem 2009; 284: 15267- 15276. https://doi.org/10.1074/jbc.M900656200

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  2. Two Calcium-Dependent Protein Kinases fromChlamydomonas reinhardtiiare transcriptionally regulated by nutrient starvation vol.9, pp.1, 2014, https://doi.org/10.4161/psb.27969