Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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HpaXm from Xanthomonas citri subsp. malvacearum is a Novel Harpin with Two Heptads for Hypersensitive Response

  • Miao, Wei-Guo (Department of Plant Pathology, Nanjing Agricultural University) ;
  • Song, Cong-Feng (Department of Plant Pathology, Nanjing Agricultural University) ;
  • Wang, Yu (Department of Plant Pathology, Nanjing Agricultural University) ;
  • Wang, Jin-Sheng (Department of Plant Pathology, Nanjing Agricultural University)
  • Published : 2010.01.31
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Abstract

A novel harpin-like protein, HpaXm, was described from cotton leaf blight bacteria, Xanthomonas citri subsp. malvacearum. The hpaXm was found to be localized between hrp2 and hrcC. A phylogenetic analysis of the complete amino acid sequence or solely the 13 highly conserved residues $H_2N$-SEKQLDQLLTQLI-COOH in the N-terminal $\alpha$-helix indicates that HpaXm is evolutionarily closer to HpaGXag and HpaXac than to Hpa1Xoo and Hpa1Xoc. A synthesized peptide containing two heptads, 39-LDQLLTQLIMALLQ-52, from the N-terminal a-helical region of HpaXm displayed comparable activity in inducing a hypersensitive response, but two other synthesized derivatives, $HpaXm{\Delta}T44C$ and $HpaXm{\Delta}M48Q$, showed reduced HR-triggering activity. The data from a GST trap test revealed that HpaXm was released into the extracellular medium, hpaXm mutant deficient for the leader peptide (1-MNSLNTQIGANSSFL-15) was unable to be secreted outside cells but still induced HR in tobacco leaves.

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References

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