Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Method Development for Electrotransformation of Acidithiobacillus caldus

  • Chen, Linxu (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Lin, Jianqun (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Li, Bing (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Lin, Jianqiang (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Liu, Xiangmei (State Key Laboratory of Microbial Technology, Shandong University)
  • Published : 2010.01.31
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Abstract

Acidithiobacillus caldus is an acidophilic, chemolithotrophic bacterium that plays an important role in bioleaching. Gene transformation into A. caldus is difficult, and only the conjugation method was reported successful, which was a relatively sophisticated method. In this research, electrotransformation of A. caldus species was achieved for the first time using A. caldus Y-3 and plasmid pJRD215. Transformants were confirmed by colony PCR specific to the str gene on pJRD215, and the recovery of the plasmid from the presumptive transformants. Optimizations were made and the transformation efficiency was increased from 0.8 to $3.6{\times}10^4$ transformants/${\mu}g$ plasmid DNA. The developed electrotransformation method was convenient in introducing foreign genes into A. caldus.

Keywords

References

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