Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Seroprevalence of Dogs with Dirofilaria immitis, Anaplasma phagocytophilum, Borrelia burgdorferi and Ehrlichia canis Infection in the Daejeon City and Kangwon Province

대전시와 강원도에서 심장사상충증, 아나플라즈마증, 보렐리아증 및 얼리키아증의 혈청학적 발생율 조사

  • Kim, Tae-Hun (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Yeon-Hee (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Choi, Jun-Hyuk (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Park, Hyung-Jin (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Chung, Dae-Wook (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Duck-Hwan (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Song, Kun-Ho (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University)
  • 김태훈 (충남대학교 수의과대학) ;
  • 김연희 (충남대학교 수의과대학) ;
  • 최준혁 (충남대학교 수의과대학) ;
  • 박형진 (충남대학교 수의과대학) ;
  • 정대욱 (충남대학교 수의과대학) ;
  • 김덕환 (충남대학교 수의과대학) ;
  • 송근호 (충남대학교 수의과대학)
  • Accepted : 2010.12.09
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to survey Dirofilaria (D.) immitis antigen, and Anaplasma (A.) phagocytophilum, Borrelia (B.) burgdorferi, and Ehrlichia (E.) canis antibodies among clinically healthy dogs in the Daejeon city and Kangwon province. Whole blood samples were collected from two hundred and fifty two dogs (127 females and 125 males, 147 outdoors and 105 indoors), and they were tested by using ELISA kit (SNAP 4Dx, IDEXX Laboratories, USA). Chi-squared analysis showed that A. phagocytophilum of female dogs in outdoors group was significantly higher prevalence than that of female dogs in indoors group (p < 0.05). In addition, infection rate of A. phagocytophilum of over 7 year-old-dogs in outdoors group revealed significantly higher prevalence than that of indoors group (p < 0.01). In E. canis, the under 4 year-old-dogs (p < 0.05), 4~7 year-old-dogs (p < 0.05) and over 7 year-old-dogs (p < 0.01) in outdoor group showed significantly higher prevalence than that of indoors group, respectively. In conclusion, this study is the large scale survey of canine D. immitis antigen, A. phagocytophilum, B. burgdorferi, and E. canis antibodies in the Daejeon city and Kangwon province, and it provides an useful reference for clinicians.

본 연구는 대전시와 강원도에서 사육중인 개 252두 (암컷: 127두, 수컷: 125두, 실외견: 147두, 실내견: 105두)를 대상으로 ELISA kit (SNAP 4Dx, IDEXX Lab. USA)를 이용하여 심장사상충증, 아나플라즈마증, 보렐리아증 및 얼리키아증에 대한 혈청학적 방법으로 발생율을 조사하였다. 아나플라즈마증은 암컷에서 실내견보다 실외견에서 유의성 있는 높은 감염율을 나타내었다. 또한 얼리키아증에서는 연령별로 분석한 결과, 실외견에서 실내견보다 4세미만, 4-7세사이, 7세 이상에서 각각 유의성 있는 높은 감염율을 나타내었다. 본 연구는 임상가들에게 유용한 임상자료로 활용될 것으로 판단된다.

Keywords

References

  1. Alleman AR and Wamsley HL. An update on anaplasmosis in dogs. (April 1, 2008) (www.veterinarymedicine.dvm360.com).
  2. Botros BA, Elmolla MS, Salib AW, Calamaio CA, Dasch GA, Arthur PR. Canine ehrlichiosis in Egypt: sero-epidemiological survey. Onderstepoort J Vet Res 1995; 62: 41-43.
  3. Chae JS, Kim CM, Kim EH, Hur EJ, Klein TA, Kang TK, Lee HC, Song JW. Molecular epidemiological study for tickborne disease (Ehrlichia canis and Anaplasma spp.) surveillance at selected U.S. military training sites/installations in Korea. Ann NY Acad Sci 2003; 990: 118-125. https://doi.org/10.1111/j.1749-6632.2003.tb07349.x
  4. Goossen HA, van den Bogaard AE, Nohlmans MK. Dogs as sentinels for human Lyme borreliosis in the Netherlands. J Clin Microbiol 2001; 39: 844-848. https://doi.org/10.1128/JCM.39.3.844-848.2001
  5. Guidoboni M, Ferreri AJ, Ponzoni M, Doglioni C, Dolcetti R. Infectious agents in mucosa-associated lymphoid tissue-type lymphomas: pathogenic role and therapeutic perspectives. Clinic Lymph Myelo 2006; 6: 289-300. https://doi.org/10.3816/CLM.2006.n.003
  6. Joppert AM, Hagiwara MK, Yoshinari NH. Borrelia burgdorferi antibodies in dogs from Cotia country, Sao Paulo State, Brazil. Rev Int Med Trop Sao Paulo 2001; 43: 251-255. https://doi.org/10.1590/S0036-46652001000500003
  7. Kim TH, Choi EH, Lee MG, Ahn SK. Serologically diagnosed Lyme disease manifesting erythema migrans in Korea. J Korean Med Sci 1999; 14: 85-88.
  8. Lee SE, Song KH, Lee SH. Survey of Ehrlichia canis and Borrelia burgdorferi antibodies in dogs (German shepherd) reared in Korea. Korean J Vet Res 2007; 47: 281-283.
  9. Lok JB. Dirofilaria sp. Taxonomy and distribution. In: Boreham PFL, RB Atwell, eds. Dirofifariasis. Florida: CRC Press. 1988: 1-28.
  10. Macieira Dde B, Messick JB, Cerquerira Ade M, Freire IM, Linhares GF, Almeida NK, Almosny NR. Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil. Vet Clin Pathol 2005; 34: 44-48. https://doi.org/10.1111/j.1939-165X.2005.tb00008.x
  11. Neer TM, Breitschwerdt EB, Greene RT, Lappin, MR. Consensus statement on ehrlichial disease of small animals from the infectious disease study group of the ACVIM. J Vet Intern Med 16 2002; 16: 309-315.
  12. Rodriguez-Vivas RI, Albornoz RE, Bolio GM. Ehrlichia canis in dogs in Yucatan, Mexico: seroprevalence, prevalence of infection and associated factors. Vet Parasitol 2005; 127: 75-79. https://doi.org/10.1016/j.vetpar.2004.08.022
  13. Song, KH, Lee SE, Hayasaki M, Shiramizu K, Kim DH, Cho KW. Seroprevalence of canine dirofilariosis in South Korea. Vet Parasitol 2003; 114: 231-236. https://doi.org/10.1016/S0304-4017(03)00137-7
  14. Theis JH, Stevens F, Theodoropoulos G, Ziedins AC. Studies on the prevalence and distribution of filariasis in dogs from Los Angeles county, California (1996-1998). Canine Pract 1999; 24: 8-16.