Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparative Study of Electron Donating Ability, Reducing Power, Antimicrobial Activity and Inhibition of ${\alpha}$-glucosidase by Sorghum bicolor Extracts

국내산 수수(Sorghum bicolor) 품종에 따른 전자공여능, 환원력, 항균활성 및 ${\alpha}$-glucosidase 저해활성의 비교

  • Sa, Yeo-Jin (Department of Applied Plant Sciences, Kangwon National University) ;
  • Kim, Ju-Sung (Oriental Bio-herb Research Institute, Kangwon National University) ;
  • Kim, Myeong-Ok (Department of Applied Plant Sciences, Kangwon National University) ;
  • Jeong, Hyun-Ju (Department of Applied Plant Sciences, Kangwon National University) ;
  • Yu, Chang-Yeon (Department of Applied Plant Sciences, Kangwon National University) ;
  • Park, Dong-Sik (Funtional Food & Nutrition Division, Rural Development Administration) ;
  • Kim, Myong-Jo (Department of Applied Plant Sciences, Kangwon National University)
  • 사여진 (강원대학교 식물자원응용공학과) ;
  • 김주성 (강원대학교 한방바이오연구소) ;
  • 김명옥 (강원대학교 식물자원응용공학과) ;
  • 정현주 (강원대학교 식물자원응용공학과) ;
  • 유창연 (강원대학교 식물자원응용공학과) ;
  • 박동식 (농촌진흥청 국립농업과학원 한식세계화연구단 기능성식품과) ;
  • 김명조 (강원대학교 식물자원응용공학과)
  • Received : 2010.04.26
  • Accepted : 2010.06.28
  • Published : 2010.10.31
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Abstract

Electron donating ability (EDA), reducing power, total phenolic contents, total flavonoid contents, antimicrobial activity, and ${\alpha}$-glucosidase inhibitory effects of twenty-two sorghum varieties were examined using ethanol extracts (70%, v/v). The electron donationg abilities (EDA) of Mesusu and Bulgeunjangmoksusu (94% at 10 ${\mu}g$/mL) indicated higher antioxidant activity compared to those of other varieties and standard antioxidants such as BHT (13%) and BHA (74%). The reducing power of Mesusu ($OD_{700}$=0.71) suggested higher antioxidant activity, which was dependent on sample concentration. Bitjarususu showed the highest content of total phenolics (22.9 mg GAE/g); however, extracts from Heuinsusu exhibited the lowest content of total phenolics (16.4 mg GAE/g). Jangmoksusu showed the highest total flavonolic contents (3.5 mg QE/g), and Sigyeongsusu and Chal (GS) susu displayed the most antibacterial activity (MIC=8 ${\mu}g$/mL) against Escherichia coli. Extracts of Bulgeunjangmoksusu, Moktaksusu, and Ginjangmoksusu showed the highest ${\alpha}$-glucosidase inhibitory effect (98%) at the concentration of 5 ${\mu}g$/mL.

수수의 기능성식품으로써 이용 가능성을 조사하기 위해 수수의 70% ethanol 추출물의 전자공여능, 환원력, 총 페놀함량, 총 플라보노이드함량, 항미생물, ${\alpha}$-glucosidase 억제활성을 측정하였다. 전자공여능 측정 결과 10 ${\mu}g$/mL 농도에서 메수수, 붉은장목 수수가 94%로 BHT(13%), BHA(74%)보다 월등히 높은 활성을 보였다. 환원력을 측정한 결과 농도가 높아짐에 따라 활성이 증가하는 경향을 보였으며, 수수 22개 품종 중에서 메수수가 0.71의 OD값으로 가장 높은 항산화 활성을 나타내었다. 추출물의 총 페놀 함량은 빗자루수수가 22.9 mg GAE/g로 높은 함량을 나타내었으며, 흰수수가 16.4 mg GAE/g로 가장 낮은 함량을 보였다. 총 플라보노이드 함량은 장목수수가 3.5 mg QE/g로 높은 함량을 보였다. 항미생물 실험 결과 E. coli에서 모든 수수가 활성을 나타내었으며, 특히 시경수수(MIC=8 ${\mu}g$/mL)와 찰(금산)수수(MIC=8 ${\mu}g$/mL)가 뛰어난 활성을 나타내었다. ${\alpha}$-Glucosidase 억제활성 측정 결과 붉은장목수수, 목탁수수, 긴장목수수가 98%로 높은 억제율을 보여주었다.

Keywords

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