Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidant activity of Persimmon Leaves during Growth

감잎의 성장시기별 항산화 효과

  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Cho, Jung-Keun (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Mok, Ji-Ye (Jeonju University Atopy & Health Research Institute) ;
  • Kim, Sang-Jun (Jeonju Biomaterials Institute) ;
  • Park, Ji-Min (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Jeon, In-Hwa (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Kim, Hyeon-Soo (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
  • 정승일 (전주생물소재연구소) ;
  • 조정근 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 목지예 (전주대학교 아토피.건강전문연구소) ;
  • 김상준 (전주생물소재연구소) ;
  • 박지민 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 전인화 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 김현수 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부)
  • Received : 2010.09.06
  • Accepted : 2010.11.10
  • Published : 2011.12.31
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Abstract

Kojongsi persimmon (Diospyros kaki) is the major cultivar of astringent persimmon in southern of Korea. Kojongsi persimmon leaf has been traditionally used for acute and chronic diseases in Oriental countries. The purpose of this study was undertaken to investigate the antioxidative activities of the extract of Kojongsi persimmon leaf during growth. We investigated the antioxidant effects of the persimmon leaf extracts during growth on total polyphenol, total flavonoid, electronic donating ability (DPPH), nitrite (NO) scavenging and superoxide dismutase (SOD)-like activity. The next, we investigated the possible cell protective effects of the persimmon extract treatment against ultraviolet B (UVB)-induced injury in HaCaT keratinocytes. The contents of total polyphenol and flavonoid in leaf extract of Kojongsi persimmon were increased in time-dependent manner. In Jun, DPPH and NO radical scavenging and SOD-like activities in the leaf extract of Kojongsi persimmon was increased to the highest. However, the antioxidant activities in persimmon varieties were not any difference. The cell cytotoxicity by UVB irradation in HaCaT keratinocytes was significantly increased with the compared to the control group. However, the treatment of leaf extract of Kojongsi persimmon in HaCaT keratinocytes was shown to protective effect against UVB-induced cell cytotoxicity. These results suggest that the leaf extract of Kojongsi persimmon has potent antioxidant activity, and protective effect against UVB-induced keratinocyte injury. Thus, these properties may be contributed in the care of acute and chronic diseases.

Keywords

References

  1. Kondo, T., Hirose, M. and Kageyama, K. (2009) Roles of oxidative stress and redox regulation in atherosclerosis. J. Atheroscler Thromb. 16: 532-538. https://doi.org/10.5551/jat.1255
  2. Mimeault, M. and Batra, S. K. (2010) Recent advances on skin-resident stem/progenitor cell functions in skin regeneration, aging and cancers and novel anti-aging and cancer therapies. J. Cell Mol. Med. 14: 116-134. https://doi.org/10.1111/j.1582-4934.2009.00885.x
  3. Geronikaki, A. A. and Gavalas, A. M. (2006) Antioxidants and inflammatory disease: synthetic and natural antioxidants with anti-inflammatory activity. Comb. Chem. High Throughput Screen. 9: 425-442. https://doi.org/10.2174/138620706777698481
  4. Kumar, S., Kuma, D., Manjusha., Saroha, K., Singh, N. and, Vashishta, B. (2008) Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm. 58: 215-220. https://doi.org/10.2478/v10007-008-0008-1
  5. 정경미, 강가화, 권민경, 송인규, 조두현, 추연대 (2004) 감잎의 품종별 화학성분과 항산화활성. 한국식품저장유통학회지 11: 175-181.
  6. 김종국, 이원영 (2002) 감잎의 성장시기에 따른 화학성분 변화. 동아시아식생활학회지 12: 32-37.
  7. Lee, M. H., Jiang, C. B., Juan, S. H., Lin, R. D. and Hou, W. C. (2006) Antioxidant and heme oxygenase-1 (HO-1)-induced effects of selected Taiwanese plants. Fitoterapia 77: 109-115. https://doi.org/10.1016/j.fitote.2005.11.012
  8. Bei, W., Peng, W., Ma, Y. and Xu, A. (2005) Flavonoids from the leaves of Diospyros kaki reduce hydrogen peroxide-induced injury of NG108-15 cells. Life Sci. 76: 1975-1988. https://doi.org/10.1016/j.lfs.2004.09.031
  9. Katsube, T., Tabata, H., Ohta, Y., Yamasaki, Y., Anuurad, E., Shiwaku, K. and Yamane, Y. (2004) Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-iocalteu assay. J. Agric. Food Chem. 52: 2391-2396. https://doi.org/10.1021/jf035372g
  10. Kotani, M., Matsumoto, M., Fujita, A., Higa, S., Wang, W., Suemura, M., Kishimoto, T. and Tanaka, T. (2000) Persimmon leaf extract and astragalin inhibit development of dermatitis and IgE elevation in NC/Nga mice. J. Allergy Clin. Immunol. 106: 159-166. https://doi.org/10.1067/mai.2000.107194
  11. Chen, G., Lu, H., Wang, C., Yamashita, K., Manabe, M., Meng, Z., Xu, S. and Kodama, H. (2002) Effect of five flavonoid compounds isolated from leaves of Diospyros kaki on stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils. Clin. Chim. Acta 326: 169-175. https://doi.org/10.1016/S0009-8981(02)00298-X
  12. Matsumoto, M., Kotani, M., Fujita, A., Higa, S., Kishimoto, T., Suemura, M. and Tanaka, T. (2002) Oral administration of persimmon leaf extract ameliorates skin symptoms and transepidermal water loss in atopic dermatitis model mice, NC/Nga. Br. J. Dermatol. 146: 221-227. https://doi.org/10.1046/j.1365-2133.2002.04557.x
  13. Bei, W., Peng, W., Ma, Y. and Xu, A. (2005) Flavonoids from the leaves of Diospyros kaki reduce hydrogen peroxideinduced injury of NG108-15 cells. Life Sci. 76: 1975-1988. https://doi.org/10.1016/j.lfs.2004.09.031
  14. Fukai, S., Tanimoto, S., Maeda, A., Fukuda, H., Okada, Y. and Nomura, M. (2009) Pharmacological activity of compounds extracted from persimmon peel (Diospyros kaki THUNB.). J. Oleo. Sci. 58: 213-219. https://doi.org/10.5650/jos.58.213
  15. Chen, X. N., Fan, J. F., Yue, X., Wu, X. R. and Li, L. T.(2008) Radical scavenging activity and phenolic compounds in persimmon (Diospyros kaki L. cv. Mopan). J. Food Sci. 73: C24-28.
  16. Peterson, D. M., Emmons, C. L and Hibbs, A. H. (2001) Phenolic antioxidants and antioxidant activity in pearling fracfractions of oat groats. J. Cereal Sci. 33: 97-103. https://doi.org/10.1006/jcrs.2000.0347
  17. Moreno, D. A., Carvajal, M., Lopez-Berenguer, C. and Garcia-Viguera, C. (2006) Chemical and biological characterisation of nutraceutical compounds of broccoli. J. Pharm. Biomed. Anal. 41: 1508-1522. https://doi.org/10.1016/j.jpba.2006.04.003
  18. Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  19. Marklund, S. and Marklund, G. (1975) Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 468-474.
  20. Gray, J. I. and Dugan, J. R. L. (1975) Inhibition of N-nitrosamine formation in model food system. J. Food Sci. 40: 981-985. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  21. Coyle, J. T. and Puttfarcken, P. (1993) Oxydative stress, glutamate and neurodegenerative disorder. Science 262: 689-695. https://doi.org/10.1126/science.7901908
  22. Chao, J., Lau, W. K., Huie, M. J., Ho, Y. S., Yu, M. S., Lai, C. S., Wang, M., Yuen, W. H., Lam, W. H., Chan, T. H. and Chang, R. C. (2010) A pro-drug of the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) prevents differentiated SH-SY5Y cells from toxicity induced by 6-hydroxydopamine. Neurosci Lett. 469: 360-364. https://doi.org/10.1016/j.neulet.2009.12.028
  23. Bengmark, S., Mesa, M. D. and Gil, A. (2009) Plant-derived health: the effects of turmeric and curcuminoids. Nutr. Hosp. 24: 273-281.
  24. Liu, W. and Wu, S. (2010) Differential roles of nitric oxide synthases in regulation of ultraviolet B light-induced apoptosis. Nitric Oxide in press.