참고문헌
- Morino K, Katsumi H, Akahori Y, Iba Y, Shinohara M, Ukai Y, Kohara Y, Kurosawa Y: Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression. J Immunol Methods 257;175-184, 2001 https://doi.org/10.1016/S0022-1759(01)00462-8
- Pack P, Pluckthun A: Miniantibodies use of amphipathic helices to produce functional, flexibly linked dimeric Fv fragments with high avidity in Escherichia coli. Biochemistry 31;1579-1584, 1992 https://doi.org/10.1021/bi00121a001
-
Horne C, Klein M, Polidoulis I, Dorrington KJ: Noncovalent association of heavy and light chains of human immunoglobulins. III. Specific interactions between
$V_H$ and$V_L$ . J Immunol 129;660-664, 1982 - Jager M, Plückthun A: Domain interactions in antibody Fv and scFv fragments: effects on unfolding kinetics and equilibria. FEBS Lett 462;307-312, 1999 https://doi.org/10.1016/S0014-5793(99)01532-X
- Jager M, Plückthun A: Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen. J Mol Biol 285;2005-2019, 1999 https://doi.org/10.1006/jmbi.1998.2425
- Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotný J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R, et al: Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single- chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci U S A 85;5879-5883, 1988 https://doi.org/10.1073/pnas.85.16.5879
- Bird RE, Hardman KD, Jacobson JW, Johnson S, Kaufman BM, Lee SM, Lee T, Pope SH, Riordan GS, Whitlow M: Single-chain antigen-binding proteins. Science 242;423-426, 1988 https://doi.org/10.1126/science.3140379
- Chaudhary VK, Queen C, Junghans RP, Waldmann TA, FitzGerald DJ, Pastan I: A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 339;394-397, 1989 https://doi.org/10.1038/339394a0
- Glockshuber R, Malia M, Pfitzinger I, Pluckthun A: A comparison of strategies to stabilize immunoglobulin Fv-fragments. Biochemistry 29;1362-1367, 1990 https://doi.org/10.1021/bi00458a002
- Bird RE, Walker BW: Single chain antibody variable regions. Trends Biotechnol 9;132-137, 1991 https://doi.org/10.1016/0167-7799(91)90044-I
- Reiter Y, Brinkmann U, Jung SH, Lee B, Kasprzyk PG, King CR, Pastan I: Improved binding and antitumor activity of a recombinant anti-erbB2 immunotoxin by disulfide stabilization of the Fv fragment. J Biol Chem 269;18327-18331, 1994
- Jung SH, Pastan I, Lee B: Design of interchain disulfide bonds in the framework region of the Fv fragment of the monoclonal antibody B3. Proteins 19;35-47, 1994 https://doi.org/10.1002/prot.340190106
- Reiter Y, Brinkmann U, Lee B, Pastan I: Engineering antibody Fv fragments for cancer detection and therapy: disulfide- stabilized Fv fragments. Nat Biotechnol 14;1239-1245, 1996 https://doi.org/10.1038/nbt1096-1239
- Reiter Y, Brinkmann U, Kreitman RJ, Jung SH, Lee B, Pastan I: Stabilization of the Fv fragments in recombinant immunotoxins by disulfide bonds engineered into conserved framework regions. Biochemistry 33;5451-5459, 1994 https://doi.org/10.1021/bi00184a014
- Reiter Y, Brinkmann U, Webber KO, Jung SH, Lee B, Pastan I: Engineering interchain disulfide bonds into conserved framework regions of Fv fragments improved biochemical characteristics of recombinant immunotoxins containing disulfide- stabilized Fv. Protein Eng 7;697-704, 1994 https://doi.org/10.1093/protein/7.5.697
- Skerra A, Pluckthun A: Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240;1038-1041, 1988 https://doi.org/10.1126/science.3285470
- Krebber A, Burmester J, Pluckthun A: Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p. Gene 178;71-74, 1996 https://doi.org/10.1016/0378-1119(96)00337-X
- Clark MA, Hammond FR, Papaioannou A, Hawkins NJ, Ward RL: Regulation and expression of human Fabs under the control of the Escherichia coli arabinose promoter, PBAD. Immunotechnol 3;217-226, 1997 https://doi.org/10.1016/S1380-2933(97)00016-X
- Knappik A, Pluckthun A: Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng 8;81-89, 1995 https://doi.org/10.1093/protein/8.1.81
- Deramn AI, Prinz WA, Belin D, Beckwith J: Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 262;1744-1747, 1993 https://doi.org/10.1126/science.8259521
- He M, Hamon M, Liu H, Kang A, Taussig MJ: Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coli. Nucleic Acids Res 23;4009-4010, 1995 https://doi.org/10.1093/nar/23.19.4009
- Jurado P, Ritz D, Beckwith J, deLorenzo V, Fernandez LA: Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J Mol Biol 320;1-10, 2002 https://doi.org/10.1016/S0022-2836(02)00405-9
- Levy R, Weiss R, Chen G, Iverson BL, Georgiou G: Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones. Protein Expr Purif 23; 338-347, 2001 https://doi.org/10.1006/prep.2001.1520
- Venturi M, Seifert C, Hunte C: High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm. J Mol Biol 315;1-8, 2002 https://doi.org/10.1006/jmbi.2001.5221
- Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning. 2nd ed. NY, USA: Cold Spring Harbor Laboratory Press Cold Spring Harbor; 1989
- Cha S, Leung PS, Coppel RL, VandeWater J, Ansari AA, Gershwin ME: Heterogeneity of combinatorial human autoantibodies against PDC-E2 and biliary epithelial cells in patients with primary biliary cirrhosis. Hepatology 20;574-583, 1994 https://doi.org/10.1002/hep.1840200305
- McCafferty J, Johnson, KS. Construction and screening of antibody display libraries. In: McCafferty J, Johnson KS, editors. Phage Display of Peptides and Proteins. A Laboratory Manual. San Diego, CA, USA: Academic Press Inc.; 1996
- Brunelle A, Schleif R: Determining residue-base interactions between AraC protein and araI DNA. J Mol Biol 209; 607-622, 1989 https://doi.org/10.1016/0022-2836(89)90598-6
- Menon KP, Lee NL: Activation of ara operons by a truncated AraC protein does not require inducer. Proc Natl Acad Sci U S A 87;3708-3712, 1990 https://doi.org/10.1073/pnas.87.10.3708
- Cha S, Leung PS, Gershwin ME, Fletcher MP, Ansari AA, Coppel RL: Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary biliary cirrhosis. Proc Natl Acad Sci U S A 90;2527-2531, 1993 https://doi.org/10.1073/pnas.90.6.2527
- O'shea EK, Rutkowski R, Stafford WE 3rd, Kim PS: Preferential heterodimer formation by isolated leucine zipper from fos and jun. Science 245;646-648, 1989 https://doi.org/10.1126/science.2503872
- Griep RA, vanTwisk C, Kerschbaumer RJ, Harper K, Torrance L, Himmler G, vanderWolf JM, Schots A: pSKAP/S: an expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins. Protein Expr Purif 16;63-69, 1999 https://doi.org/10.1006/prep.1999.1041
- Hayhurst A: Improved expression characteristics of single- chain Fv fragments when fused downstream of the Escherichia coli maltose-binding protein or upstream of a single immunoglobulin-constant domain. Protein Expr Purif 18;1-10, 2000 https://doi.org/10.1006/prep.1999.1164
- Bustos SA, Schleif RF: Functional domains of the AraC protein. Proc Natl Acad Sci U S A 90:5638-5642, 1993 https://doi.org/10.1073/pnas.90.12.5638
- Strachan G, Williams S, Moyle SP, Harris WJ, Porter AJ: Reduced toxicity of expression, in Escherichia coli, of anti pollutant antibody fragments and their use as sensitive diagnostic molecules. J Appl Microbiol 87;410-417, 1999 https://doi.org/10.1046/j.1365-2672.1999.00834.x
- Bessette PH, Aslund F, Beckwith J, Georgiou G: Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci U S A 96;13703-13708, 1999 https://doi.org/10.1073/pnas.96.24.13703