Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Calcium Mobilization Inhibits Lipid Accumulation During the Late Adipogenesis via Suppression of PPARγ and LXRα Signalings

  • Kim, Seung-Jin (Dept. of Biological sciences, College of Natural sciences, Chonnam National University) ;
  • Choi, Ho-Jung (Dept. of Biological sciences, College of Natural sciences, Chonnam National University) ;
  • Jung, Chung-Hwan (Dept. of Biological sciences, College of Natural sciences, Chonnam National University) ;
  • Park, Sung-Soo (Dept. of Biological sciences, College of Natural sciences, Chonnam National University) ;
  • Cho, Seung-Rye (Dept. of Biological sciences, College of Natural sciences, Chonnam National University) ;
  • Oh, Se-Jong (Division of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Eung-Seok (Dept. of Biological sciences, College of Natural sciences, Chonnam National University)
  • Received : 2010.04.11
  • Accepted : 2010.10.14
  • Published : 2010.10.31
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Abstract

Calcium plays a role as a signaling molecule in various cellular events. It has been reported that calcium suppresses adipocyte differentiation only in the early phase of adipogenesis. Herein, we demonstrate that treatment of A23187, a mobilizer of intracellular calcium, on day 4 post adipocyte differentiation could still reduce lipid accumulation in differentiating 3T3-L1 cells for 48 h. In addition, luciferase reporter gene and RT-Q-PCR assays demonstrate that A23187 can selectively inhibit transcriptional activities and expression of PPAR$\gamma$ and LXR$\alpha$, suggesting that A23187 may reduce lipid accumulation in the late phase of adipogenesis via downregulation of PPAR$\gamma$ and LXR$\alpha$ expression and transactivation. Moreover, inhibition of HDAC activity by trichostatin A (TSA) partially blocked A23187-mediated downregulation of transcriptional activities of PPAR$\gamma$ and LXR$\alpha$. Together, our data demonstrate that calcium mobilization inhibits expression and transcriptional activities of PPAR$\gamma$ and LXR$\alpha$, resulting in reduced lipid accumulation in differentiating adipocytes, and thus, mobilization of intracellular calcium in adipocytes may serve as a new preventive and therapeutic approach for obesity.

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References

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